A5102986625- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Regulation of Appetite and Obesity
- Metabolism, Diabetes, and Cancer
- Protein Kinase Regulation and GTPase Signaling
- Estrogen and related hormone effects
- Signaling Pathways in Disease
- Aldose Reductase and Taurine
- Pancreatic function and diabetes
- Drug Transport and Resistance Mechanisms
- Nerve injury and regeneration
- Antioxidant Activity and Oxidative Stress
- Neurobiology and Insect Physiology Research
- Cellular transport and secretion
- Muscle metabolism and nutrition
- Neuroscience and Neuropharmacology Research
- Eicosanoids and Hypertension Pharmacology
- RNA Research and Splicing
- Gastroesophageal reflux and treatments
- Ultrasound and Hyperthermia Applications
- Cancer Risks and Factors
University of Florida
2023
Tianjin Medical University
2022
Scripps Research Institute
2016-2019
Discovery Institute
2018
Sanford Burnham Prebys Medical Discovery Institute
2011-2018
Wuhan General Hospital of Guangzhou
2014-2018
General Hospital of Guangzhou Military Command
2014-2017
Academy of Military Medical Sciences
2015
Czech Academy of Sciences, Institute of Biotechnology
2014
Institute of Biophysics
2007-2009
STIM1 and Orai1 are essential components of Ca(2+) release-activated channels (CRACs). After endoplasmic reticulum store depletion, in the aggregates migrates toward cell periphery to co-localize with on opposing plasma membrane. Little is known about roles different domains protein clustering, migration, interaction, and, ultimately, opening CRAC channels. Here we demonstrate that coiled-coil domain C terminus crucial for its aggregation. Amino acids 425-671 STIM1, which contain a...
Estrogen receptors ERα and ERβ share considerable sequence homology yet exert opposite effects on breast cancer cell proliferation. While the proliferative role of in tumors is well characterized, it not clear whether antitumor activity can be mobilized cells. Here, we have shown that phosphorylation a tyrosine residue (Y36) present ERβ, but ERα, dictates ERβ-specific activation transcription required for ERβ-dependent inhibition growth culture murine xenografts. Additionally, c-ABL kinase...
Stress can be a motivational force for decisive action and adapting to novel environment; whereas, exposure chronic stress contributes the development of depression anxiety. However, molecular mechanisms underlying stress-responsive behaviors are not fully understood. Here, we identified orphan receptor GPR158 as regulator operating in prefrontal cortex (PFC) that links depression. is highly upregulated PFC human subjects with major depressive disorder. Exposure mice also increased protein...
The cationic nature of cell penetrating peptides (CPPs) and their absence selectivity restrains applications in vivo. In this work, polymer nanoparticles (NPs) modified with photo- pH-responsive polypeptides (PPPs) were successfully developed respond to near-infrared (NIR) light illumination at the tumor site a lowered extracellular pH (pHe). PPPs, internalization function CPPs (positively charged) is quenched by pH-sensitive inhibitory peptide (negatively charged), which linked via...
Therapeutic outcome for the treatment of glioma was often limited due to non-targeted nature drugs and physiological barriers, including blood-brain barrier (BBB) tumor (BBTB). An ideal glioma-targeted delivery system must be sufficiently potent cross BBB BBTB then target cells with adequate optimized physiochemical properties biocompatibility. However, it is an enormous challenge researchers engineer above-mentioned features into a single nanocarrier particle. New frontiers in nanomedicine...
Therapeutic outcome for the treatment of glioma was often limited due to non-targeted nature and low permeability drugs across blood-brain barrier (BBB). An ideal glioma-targeted delivery system need traverse BBB then target cells with adequate optimized physiochemical properties biocompatibility. However, it is an enormous challenge researchers engineer above-mentioned features into a single nanocarrier particle. New frontiers in nanomedicine are advancing research new biomaterials. In this...
Anxiety disorders are associated with body weight changes in humans. However, the mechanisms underlying anxiety-induced remain poorly understood. Using Emx1Cre/+ mice, we deleted gene for brain-derived neurotrophic factor (BDNF) cortex, hippocampus, and some amygdalar subregions. The resulting mutant mice displayed impaired GABAergic transmission elevated anxiety. They were leaner when fed either a chow diet or high-fat diet, owing to higher sympathetic activity, basal metabolic rate, brown...
To specifically deliver cytotoxic drug to tumor cells and enhance cellular uptake is the key for effective cancer therapy. In this paper, we described a novel targeting system, which designed combine features of biological (cell-penetrating peptides, CPPs) physical (magnetic) use in magnetic hyperthermia-triggered release. A doxorubicin-CPPs conjugate (DOX-CPPs) was loaded into thermosensitive liposomes (TSMLs) (DOX-CPPs/TSMLs), vitro DOX-CPPs release activity, anti-proliferation effect,...
Estrogen receptors (ERalpha and ERbeta) are ligand-regulated transcription factors that play critical roles in the development progression of breast cancer by regulating target genes involved cellular proliferation. The transcriptional activity ERalpha ERbeta is known to be modulated cofactor proteins. We used a yeast two-hybrid system identified NFAT3 as novel ERbeta-binding protein. interacted with both vitro mammalian cells ligand-independent fashion. bound specifically region containing...
The conjugation of tunable peptides or materials with nanocarriers represents a promising approach for drug delivery to tumor cells. In this study, we report the development novel liposomal carrier system that exploits cell surface binding synergism between photo-sensitive (PSPs) and targeting ligands. positive charges lysine residues on cell-penetrating (CPPs) were temporarily caged by photolabile-protective groups (PG), thereby forming PSP. Furthermore, PSP enhances specific uptake into...
Tumor-oriented nanocarrier drug delivery approaches with photo-sensitivity have been drawing considerable attention over the years. Here we described a nanostructured lipid carrier (NLC) modified photo-responsive cell-penetrating peptides (pCPP-NLC). The conventional cell penetrating peptide (CPP)-mediated intracellular system sometimes seemed limited due to lack of target selectivity activity. In this study, pCPP (CKRRMK(Nvoc)WK(Nvo0c)K(Nvoc)), CPP originated from (CKRRMKWKK), was endowed...
Insulin stimulates GLUT4 (glucose transporter 4) translocation in adipocytes and muscles. An emerging picture is that Rab10 could bridge the gap between insulin signalling cascade adipocytes. In present study, two potential effectors of Rab10, GDI (guanine-nucleotide-dissociation inhibitor)-1 GDI-2, are characterized respect to their roles insulin-stimulated translocation. It shown both GDI-1 GDI-2 exhibit similar distribution at TGN (trans-Golgi network) periphery structures. Meanwhile,...
Light is an ideal general triggered signal, which occurs as a result of its non-invasive nature, desirable controllability and high spatial resolution. However, due to low penetrability ability harm tissues, the use ultraviolet (UV) light for nanocarrier release in vivo applications has been limited. Compared with UV light, near-infrared (NIR) deeply penetrates tissues less damaging cells. In this study, we have devised tested strategy site-specific delivery small interfering RNA (siRNA)...
Abstract Objectives To enhance the targeting delivery efficiency of small interfering RNA (siRNA) to tumour cells, a novel multifunctional liposome (PSP/NGR-L) comodified with photo-sensitive cell-penetrating peptides (PSP) and asparagine-glycine-arginine peptide (NGR) was constructed investigated. Methods PSP conjugated 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-maleimide(polyethylene glycol)-2000 (DSPE-PEG2000-MAL) generate DSPE-PEG2000-PSP used form PSP/NGR-L, features liposomes...