A5100387631- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Pluripotent Stem Cells Research
- Immune cells in cancer
- Advanced biosensing and bioanalysis techniques
- Retinal Development and Disorders
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Anesthesia and Neurotoxicity Research
- Virus-based gene therapy research
- Neurogenesis and neuroplasticity mechanisms
- Prenatal Screening and Diagnostics
- interferon and immune responses
- Cancer Immunotherapy and Biomarkers
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- MicroRNA in disease regulation
- RNA regulation and disease
- 3D Printing in Biomedical Research
- Reproductive Biology and Fertility
- Renal and related cancers
- Retinal Diseases and Treatments
- Neuroscience and Neural Engineering
- Tryptophan and brain disorders
- Reproductive biology and impacts on aquatic species
- Supramolecular Self-Assembly in Materials
Wenzhou Medical University
2018-2025
Affiliated Eye Hospital of Wenzhou Medical College
2019-2025
Gilead Sciences (United States)
2022-2025
Shandong Provincial Hospital
2024-2025
Zhejiang Lab
2024-2025
Sichuan University
2019-2025
State Key Laboratory of Oral Diseases
2025
Huazhong University of Science and Technology
2016-2024
Wannan Medical College
2024
Tianjin Medical University
2004-2024
The initial cases of novel coronavirus (2019-nCoV)-infected pneumonia (NCIP) occurred in Wuhan, Hubei Province, China, December 2019 and January 2020. We analyzed data on the first 425 confirmed Wuhan to determine epidemiologic characteristics NCIP.We collected information demographic characteristics, exposure history, illness timelines laboratory-confirmed NCIP that had been reported by 22, described estimated key time-delay distributions. In early period exponential growth, we epidemic...
Somatic cells can be reprogrammed to induced pluripotent stem (iPSCs) by expressing four transcription factors: Oct4, Sox2, Klf4, and c-Myc. Here we report that enhancing RA signaling receptors (RARs) or agonists profoundly promoted reprogramming, but inhibiting it using a RAR-α dominant-negative form completely blocked it. Coexpressing Rarg (RAR-γ) Lrh-1 (liver receptor homologue 1; Nr5a2) with the factors greatly accelerated reprogramming so of mouse embryonic fibroblast ground-state iPSCs...
Abstract Cardiac aging, particularly cardiac cell senescence, is a natural process that occurs as we age. Heart function gradually declines in old age, leading to continuous heart failure, even people without prior history of disease. To address this issue and improve function, it crucial investigate the molecular mechanisms underlying senescence. This review summarizes main key proteins involved further discusses modulators cellular senescence aging hearts. Furthermore, discussion will...
Sexual fate is no longer seen as an irreversible deterministic switch set during early embryonic development but ongoing battle for primacy between male and female developmental trajectories. That sexual not final must be actively maintained via continuous suppression of the opposing network creates potential flexibility into adulthood. In many fishes, sexuality only extremely plastic, sex change a usual adaptive part life cycle. Sequential hermaphrodites begin one sex, changing sometime...
Rationale: Mitochondrial disorders preferentially affect tissues with high energy requirements, such as the retina and corneal endothelium, in human eyes. Mesenchymal stem cell (MSC)-based treatment has been demonstrated to be beneficial for ocular degeneration. However, aside from neuroprotective paracrine actions, mechanisms underlying effect of MSCs on retinal are largely unknown. In this study, we investigated fate associated characteristics mitochondria subjected intercellular transfer...
Significance As a genetic malignancy, retinoblastoma (Rb) is caused by RB1 mutations; however, its developmental origin and drug agents for human Rb remain largely unexplored. Here we describe an innovative organoid model derived from embryonic stem cells with biallelic mutagenesis of the gene. We identify tumorigenic growth in organoids, as well properties consistent primary Rb. confirm that cell stemmed ARR3 + maturing cone precursor SYK inhibitors displaying significant therapeutic...
Abstract Engineering of the Cpf1 crRNA has potential to enhance its gene editing efficiency and non-viral delivery cells. Here, we demonstrate that extending length at 5′ end can both in cells vivo. Extending enhances RNP induce non-homologous end-joining homology-directed repair using electroporation Additionally, chemical modifications on extended result enhanced serum stability. Also, by 59 nucleotides increases vivo cationic vehicles including polymer nanoparticle. Thus, extension...
Abstract The clustered regularly interspaced short palindromic repeats (CRISPR)/associated nuclease (Cas) system is an efficient gene editing tool. In this study, it found that both single guide RNA (gRNA) and Cas9 protein could be exported from the CRISPR/Cas9‐expressing cells by endogenous exosomes independently. Further experiments demonstrate these naturally produced used as a vehicle to deliver functional hepatitis B virus (HBV)‐specific gRNA cut HBV DNA transfected in HuH7 or human...
Triple-negative breast cancer (TNBC) represents the most aggressive subtype of with an extremely dismal prognosis and few treatment options. As a desmoplastic tumor, TNBC tumor cells are girdled by stroma composed cancer-associated fibroblasts (CAFs) their secreted stromal components. The rapidly proliferating cells, together stroma, exert additional solid tissue pressure on vasculature surrounding tissues, severely obstructing therapeutic agent from deep intratumoral penetration, resulting...
To describe a micromanipulation-electrofusion procedure for transferring germinal vesicles (GVs) between immature human oocytes.Pilot study to assess oocyte maturation after an invasive micromanipulation procedure.Research laboratory at university medical center.Immature oocytes were discarded from intracytoplasmic sperm injection (ICSI)-IVF cycles of patients 23-48 years age.Initially, GV removal and transfer performed on the same oocyte; these "self-reconstructed" then cultured in vitro up...
Survivin, which is the smallest member of inhibitor apoptosis protein (IAP) family, a chromosomal passenger that mediates spindle assembly checkpoint and cytokinesis, also functions as an apoptosis. Frequently overexpressed in human cancers not expressed most adult tissues, survivin has been proposed attractive target for anticancer therapies and, some cases, even touted cancer-specific gene. Survivin is, however, proliferating cells, including hematopoietic stem T-lymphocytes, erythroid...
Primary ciliary dyskinesia (PCD) is an inherited chronic respiratory obstructive disease with randomized body laterality and infertility, resulting from cilia sperm dysmotility. PCD characterized by clinical variability extensive genetic heterogeneity, associated different ultrastructural defects mutations identified in >20 genes. Next generation sequencing (NGS) technologies therefore present a promising approach for diagnosis which not yet routine use. We developed targeted panel-based NGS...
Herpes simplex encephalitis (HSE) caused by herpes virus 1 (HSV-1) infection can lead to a high mortality rate and severe neurological sequelae. The destruction of the blood-brain barrier (BBB) is an important pathological mechanism for development HSE. However, specific underlying BBB remains unclear. Our previous study found that Golgi apparatus (GA) plays crucial role in maintaining integrity BBB. Therefore, this present aimed investigate GA its mechanisms. Mouse brain endothelial cells...
Official recommendations differ regarding tympanostomy-tube placement for children with recurrent acute otitis media.
Tumor recurrence is the major obstacle for pushing envelope of liver transplantation hepatocellular carcinoma (HCC) patients. The inflammatory cascades activated by acute graft injury promote tumor recurrence. We aimed to explore role and mechanism myeloid-derived suppressor cell (MDSC) mobilization induced on By analyzing 331 HCC patients who received transplantation, with weight ratio (GWR, divided estimated standard recipient) <60% had higher than GWR ≥60% ones. MDSCs CXCL10/TLR4 levels...
Wee1 inhibition has received great attention in the past decade as a promising therapy for cancer treatment. Therefore, potent and selective inhibitor is highly desirable. Our efforts to make safer more efficacious inhibitors led discovery of compound 16, with balanced potency, ADME, pharmacokinetic properties. The chiral ethyl moiety 16 provided an unexpected improvement potency. Compound known ZN-c3, showed excellent vivo efficacy currently being evaluated phase 2 clinical trials.