A5060135800- Hepatitis B Virus Studies
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- MicroRNA in disease regulation
- Cancer-related Molecular Pathways
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- Renal and related cancers
- Cytokine Signaling Pathways and Interactions
- RNA modifications and cancer
- Circular RNAs in diseases
- RNA Research and Splicing
- Cancer, Lipids, and Metabolism
- Hepatitis Viruses Studies and Epidemiology
- CRISPR and Genetic Engineering
- Endoplasmic Reticulum Stress and Disease
- RNA Interference and Gene Delivery
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer-related gene regulation
- Genetics, Aging, and Longevity in Model Organisms
- interferon and immune responses
- Protease and Inhibitor Mechanisms
- Cancer, Hypoxia, and Metabolism
- Hepatocellular Carcinoma Treatment and Prognosis
Peking University
2016-2025
Chinese PLA General Hospital
2014-2025
Inner Mongolia University of Technology
2024-2025
Mongolian University of Science and Technology
2025
Sun Yat-sen University
2025
Beijing Ditan Hospital
2018-2024
State Key Laboratory of Kidney Diseases
2011-2024
Capital Medical University
2018-2024
Chinese People's Liberation Army
2009-2024
People's Liberation Army No. 150 Hospital
2006-2024
Biocompatible carboxyethyl chitosan/poly(vinyl alcohol) (CECS/PVA) nanofibers were successfully prepared by electrospinning of aqueous CECS/PVA solution. The composite nanofibrous membranes subjected to detailed analysis scanning electron microscopy (SEM), differential calorimetry (DSC), and X-ray diffraction (XRD). SEM images showed that the morphology diameter mainly affected weight ratio CECS/PVA. XRD DSC demonstrated there was strong intermolecular hydrogen bonding between molecules CECS...
Hepatitis B virus (HBV) RNA in serum has recently been linked to efficacy and prognosis of chronic hepatitis (CHB) treatment. This study explored the nature, origin, underlying mechanisms, potential clinical significance HBV RNA.The levels DNA were determined supernatant induced HepAD38, HBV-expressing HepG2.2.15 cells primary human hepatocytes (PHH), transgenic mice CHB patients. NP-40 proteinase K treatment, sucrose density gradient centrifugation, electron microscopy, northern blot,...
Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSC-EVs) have been recognized as a promising cell-free therapy for acute kidney injury (AKI), which avoids safety concerns associated with direct cell engraftment. However, low stability and retention of MSC-EVs limited their therapeutic efficacy. RGD (Arg-Gly-Asp) peptide binds strongly to integrins, identified on the surface MSC-EV membranes; yet has not applied EV scaffolds enhance prolong bioavailability. Here, we...
Objective: To explore and analyze the possible mechanism of liver injury in patients with coronavirus disease 2019 (novel pneumonia, NCP). Methods: The correlation between ALT, AST other enzyme changes condition NCP patients' status reported literature was comprehensively analyzed. ACE2 expression tissue for novel analyzed based on single cell sequencing (GSE115469) data. RNA-Seq method used to Ace2 transcription factors related its tissues at various time-points after hepatectomy mouse...
The molecular basis for aging of the kidney is not well understood. MicroRNAs (miRNAs) contribute to processes such as development, differentiation, and apoptosis, but their contribution process unknown. Here, we analyzed miRNA expression profile young (3-month) old (24-month) rat kidneys identified biologic pathways genes regulated by differentially expressed miRNAs. We observed upregulation 18 miRNAs with aging, mainly regulating associated energy metabolism, cell proliferation,...
The purpose of this study was to investigate the feasibility using quantitative shear wave elastography for assessing functional integrity Achilles tendon and summarize changes in elasticity ruptured tendons comparison with normal controls.Thirty-six 14 were examined coupled a linear array transducer (4-15 MHz). value each longitudinal view measured.The mean ± SD 291.91 4.38 kPa (note that there are saturated measurement phenomena tendon, so actual will be >300 kPa), whereas had an 56.48...
Abstract BACKGROUND: Recent research has suggested that the oncomir microRNA 155 (miR‐155) is up‐regulated in hepatocellular carcinoma (HCC). In this study, authors investigated tumorigenic mechanism of development HCC. METHODS: Quantitative reverse transcriptase‐polymerase chain reaction (RT‐PCR) was conducted to analyze expressions miR‐155 and its potential target genes paired tumor tissues adjacent tumor‐free disease‐free liver tissue samples. The silico predicted were assessed by...
Background & AimsAlthough hepatitis B virus (HBV) integration into the human genome has been considered as one of major causative factors to hepatocarcinogenesis, underlying mechanism(s) was still elusive. Here we investigate essential difference(s) HBV between HCC tumor and adjacent non-tumor tissues explore factor(s) that determine oncogenicity integration.Methods1115 sites were collected from four recent studies. Functional annotation analysis targeted host genes (ITGs) performed using...
Abstract The clustered regularly interspaced short palindromic repeats (CRISPR)/associated nuclease (Cas) system is an efficient gene editing tool. In this study, it found that both single guide RNA (gRNA) and Cas9 protein could be exported from the CRISPR/Cas9‐expressing cells by endogenous exosomes independently. Further experiments demonstrate these naturally produced used as a vehicle to deliver functional hepatitis B virus (HBV)‐specific gRNA cut HBV DNA transfected in HuH7 or human...
Abstract Endothelial cell injury plays a critical part in ischemic acute kidney (AKI) and participates the progression of AKI. Targeting renal endothelial therapy may ameliorate vascular further improve prognosis Here, P‐selectin as biomarker AKI cells is identified binding peptide (PBP)‐engineered extracellular vesicles (PBP‐EVs) with imaging therapeutic functions are developed. The results show that PBP‐EVs exhibit selective targeting tendency to injured kidneys, while providing...
The AU-rich binding factor 1 (AUF1) is one of the well known adenylate-uridylate-rich element (ARE)-specific RNA-binding proteins (ARE-BPs) for which dysregulation has been reported in various human cancers. However, involvement AUF1 initiation and progression hepatocellular carcinoma (HCC) still elusive. In this study, we aimed at exploring clinical significance, function, mechanism abnormal expression HCC. Using a bioinformatics analysis Cancer Genome Atlas (TCGA) Liver Institute (LCI)...
Abstract Introduction The prevalence of renal fibrosis is higher in older than younger individuals. Through paracrine activity, bone marrow mesenchymal stem cell-derived microvesicles (BM-MSC-MVs) influence the process fibrosis. Differences microRNA (miRNA) expression BM-MSC-MVs that correlate with age subjects and correlation between miRNA have not been established. present study aimed to analyze differences young or rats its on tumor growth factor-beta 1 (TGF-β1)-mediated...
Mesenchymal stem cell (MSC)–derived extracellular vesicles (EVs) have been shown to stimulate regeneration in the treatment of kidney injury. Renal is also thought be stimulated by activation Sox9+ cells. However, whether and how mechanisms underlying EV cell–dependent intersect unclear. We reasoned that a high-resolution imaging platform living animals could help untangle this system. To test idea, we first applied EVs derived from human placenta–derived MSCs (hP-MSCs) Sox9-CreERT2; R26mTmG...
A biocomposite of hydroxyapatite (HAp) with electrospun nanofibrous scaffolds was prepared by using chitosan/polyvinyl alcohol (CS/PVA) and N-carboxyethyl chitosan/PVA (CECS/PVA) membranes as organic matrix, HAp formed in supersaturated CaCl2 KH2PO4 solution. The influences carboxylic acid groups CECS/PVA fibrous scaffold polyanionic additive poly(acrylic acid) (PAA) the incubation solution on crystal distribution were investigated. Field-emission scanning electron microscopy (FE-SEM),...
To examine the role of hepatitis B virus (HBV) integration in hepatocarcinogenesis, a systematic comparative study both tumor and their corresponding non-tumor derived tissue has been conducted cohort 60 HBV associated hepatocellular carcinoma (HCC) patients. By using Alu-polymerase chain reaction (PCR) ligation-mediated PCR, 233 viral-host junctions mapped across all human chromosomes at random, no difference between was observed, with exception fragile sites (P = 0.0070). insertions close...
Aberration of miR-129-2 has been linked to a variety human tumours. However, whether is involved in hepatocarcinogenesis remains unknown. Here, we investigate the involvement HBV infection-related HCC. A total 75 paired tumour and their corresponding non-tumour liver tissues from HCC patients with serum HBsAg positive were collected. The methylation gene was quantitatively analysed by DNA methylation-sensitive endonuclease digestion followed quantitative PCR. expression mature (miR-129-3p)...
Integration of human papillomavirus (HPV) viral DNA into the genome has been postulated as an important etiological event during cervical carcinogenesis. Several recent reports suggested a possible role for such integration‐targeted cellular genes (ITGs) in Therefore, comprehensive analysis HPV integration events was undertaken using data collected from 14 publications, with 499 loci on chromosomes included. It revealed that preferred to integrate intragenic regions and gene‐dense...