A5033269770- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- Plant Pathogenic Bacteria Studies
- Plant Molecular Biology Research
- DNA Repair Mechanisms
- Plant nutrient uptake and metabolism
- Cardiac Structural Anomalies and Repair
- HER2/EGFR in Cancer Research
- Connective tissue disorders research
- Plant Reproductive Biology
- Protein Kinase Regulation and GTPase Signaling
- Effects of Radiation Exposure
- Yeasts and Rust Fungi Studies
- Plant-Microbe Interactions and Immunity
- DNA and Nucleic Acid Chemistry
- Advanced Data Storage Technologies
- Neural Networks and Applications
- Insect symbiosis and bacterial influences
- Cardiovascular Effects of Exercise
- Cardiomyopathy and Myosin Studies
- Plant Pathogens and Fungal Diseases
- Neurogenetic and Muscular Disorders Research
- Plant Micronutrient Interactions and Effects
University of Florida
2022-2025
Florida Department of Citrus
2022-2025
Hebei University of Engineering
2024
Second Affiliated Hospital of Zhengzhou University
2021
Northwest A&F University
2017-2020
State Key Laboratory of Genetic Engineering
2015-2018
Fudan University
2015-2018
PLA 306 Hospital
2016
Radiation Oncology Associates
2014
Citrus canker caused by Xanthomonas citri subsp. (Xcc) is a destructive citrus disease worldwide. Generating disease-resistant cultivars the most effective, environmentally friendly and economic approach for control. However, traditional breeding lengthy laborious. Here, we develop transgene-free canker-resistant sinensis lines in T0 generation within 10 months through transformation of embryogenic protoplasts with Cas12a/crRNA ribonucleoprotein to edit susceptibility gene CsLOB1. Among 39...
Estrogen receptors ERα and ERβ share considerable sequence homology yet exert opposite effects on breast cancer cell proliferation. While the proliferative role of in tumors is well characterized, it not clear whether antitumor activity can be mobilized cells. Here, we have shown that phosphorylation a tyrosine residue (Y36) present ERβ, but ERα, dictates ERβ-specific activation transcription required for ERβ-dependent inhibition growth culture murine xenografts. Additionally, c-ABL kinase...
Abstract Nitrogen (N) is a limiting nutrient for plant growth and productivity. The phytohormone abscisic acid (ABA) has been suggested to play vital role in nitrate uptake fluctuating N environments. However, the molecular mechanisms underlying involvement of ABA deficiency responses are largely unknown. In this study, we demonstrated that signaling components, particularly three subclass III SUCROSE NON‐FERMENTING1 (SNF1)‐RELATED PROTEIN KINASE 2S (SnRK2) proteins, function root foraging...
Citrus canker caused by Xanthomonas citri subsp. (Xcc) is an economically important disease in most citrus production regions worldwide. Xcc secretes a transcriptional activator like effector (TALE) PthA4 to bind the binding elements (EBEs) promoter region of susceptibility gene LOB1 activate its expression, which turn causes symptoms. Editing EBE with Cas9/gRNA has been used generate resistant plants. However, EBE-edited lines generated contain indels 1-2 bp, higher possibility be overcome...
Agrobacterium-mediated transient expression (AMTE) is an important tool in plant genetics study and biotechnology. AMTE remains problematic citrus many species. Previous has shown that pretreatment of leaves with Xanthomonas citri subsp. (Xcc), which causes canker, significantly improves the efficacy. Here we have Xcc promotes mainly through triggering cell division upregulating wall degrading enzymes. We demonstrate via PthA, a transcription activator-like effector (TALE) known to trigger...
Meiotic recombination is required for proper homologous chromosome segregation in plants and other eukaryotes. The eukaryotic RAD51 gene family has seven ancient paralogs with important roles mitotic meiotic recombination. Mutations mammalian homologs RAD51C XRCC3 lead to embryonic lethality. In the model plant Arabidopsis thaliana, are not essential vegetative development but each somatic recombination, mechanism of unclear. non-lethal rad51c xrcc3 null mutants provide an opportunity study...
The downy mildew disease in grapevines is caused by Plasmopara viticola. This poses a serious threat wherever viticulture practiced. Wild Vitis species showing resistance to P. viticola offer promising pathway develop new grapevine cultivars resistant which will allow reduced use of environmentally unfriendly fungicides. Here, transmission and scanning microscopy was used compare the responses three genotypes V. davidii var. cyanocarpa, piasesezkii pseudoreticulata suceptible vinifera...
Meiosis halves diploid genomes to haploid and is essential for sexual reproduction in eukaryotes. Meiotic recombination ensures physical association of homologs their subsequent accurate segregation results the redistribution genetic variations among progeny. Most organisms have two classes cross-overs (COs): interference-sensitive (type I) -insensitive II) COs. DNA synthesis meiotic recombination, but whether has a role differentiating CO pathways unknown. Here, we show that Arabidopsis...
The CRISPR/Cas9 system has been widely used for generating targeted mutations in various species. In Arabidopsis, it largely relies on the edited cells where Cas9 protein performs its activity to obtain heritable and stable mutated lines. Here, we designed an improved system, named as MSC (meiocyte-specific CRISPR/Cas9) which expression is driven by experimentally approved meiocyte-specific promoter (AtDMC1 promoter). Two endogenous genes, including vegetative gene AtDET2 reproductive...
For partial differential equations on domains of arbitrary shapes, existing works neural operators attempt to learn a mapping from geometries solutions. It often requires large dataset geometry-solution pairs in order obtain sufficiently accurate operator. However, for many industrial applications, e.g., engineering design optimization, it can be prohibitive satisfy the requirement since even single simulation may take hours or days computation. To address this issue, we propose reference...
Citrus canker, caused by
Abstract Citrus canker, caused by Xanthomonas citri subsp. ( Xcc ), is a devastating disease worldwide. Previously, we successfully generated canker-resistant sinensis cv. Hamlin lines in the T0 generation, achieving mutation efficiency of 97.4%. This was achieved through transformation embryogenic protoplasts using Cas12a/1 crRNA ribonucleoprotein (RNP) system to edit canker susceptibility gene, CsLOB1 , which led small indels. Here, transformed with Cas12a/3 crRNAs RNP, resulting 100%...
Background/Objectives: Nitrogen is an essential macroelement for plant growth and productivity. Calcium (Ca2+) acts as a critical second messenger in numerous adaptations developmental processes plants. The Calcineurin B-like protein (CBL)-interacting kinase (CIPK) signaling pathway has been demonstrated to be involved multiple intracellular ion homeostasis of plants response stresses. However, whether CIPKs are nitrate deficiency stress remains largely unknown. Methods: In this study, we...
Marfan syndrome (MFS) is a connective-tissue disorder caused mainly by heterozygous mutations in the FBN1 gene that encodes fibrillin-1. In this study, human induced pluripotent stem cell (iPSC) line ZZUSAHi003-A was generated from peripheral blood mononuclear cells (PBMCs) isolated female patient with MFS using non-integrative Sendai virus. The iPSC carried mutation, showed normal karyotype, expressed pluripotency markers and had capacity to differentiate into three germ layers vivo. This...
The FLNC gene encodes the sarcomeric protein filamin C which plays a central role in cardiomyocytes. Truncating mutations (stop or frameshift etc.) also cause dilated cardiomyopathy (DCM) and arrhythmogenic right ventricular (ARVC). To further understand exact of DCM, we have generated human knockout cell line from original embryonic stem H9 by CRISPR/Cas9 editing technology this study. establishment WAe009-A-70 compound heterozygous 4 bp deletion/13 deletion exon 1 resulted translation...
Abstract Citrus is one of the top three tree crops in world and its production faces many devastating disease challenges such as citrus canker caused by Xanthomonas citri subsp. (Xcc). Genetic improvement via traditional approaches a lengthy (approximately 20 years) laborious process. Biotechnological including CRISPR genome editing technologies have shown promise. However, none plants generated biotechnological been commercialized, which primarily resulted from transgenic nature genetically...