Soybean is one of the most important crops, providing large amounts dietary proteins and edible oil, also an excellent model for studying evolution duplicated genes. However, relative to plants Arabidopsis rice, present knowledge about soybean transcriptome quite limited. In this study, we employed RNA-seq investigate transcriptomes 11 tissues, genome-wide discovery truly expressed genes, novel alternative transcripts, as well analyses conservation divergence genes their functional...

Abstract Cold and drought stresses seriously affect cassava ( Manihot esculenta ) plant growth yield. Recently, long noncoding RNAs (lncRNAs) have emerged as key regulators of diverse cellular processes in mammals plants. To date, no systematic screening lncRNAs under abiotic stress their regulatory roles has been reported. In this study, we present the first reference catalog 682 high-confidence based on analysis strand-specific RNA-seq data from shoot apices young leaves cold, control...

A photoredox/nickel dual-catalyzed decarboxylative cross-coupling reaction of anomeric ribosyl/deoxyribosyl acids with aryl/heteroaryl bromides has been developed. The proceeds smoothly under visible-light irradiation and features the using cost-effective easily handled catalysts starting materials, which allows highly stereoselective synthesis diverse aryl/heteroaryl-C-nucleosides in moderate to high yields.

Alternative splicing is an essential post-transcriptional regulatory mechanism that can impact mRNA stability and protein diversity of eukaryotic genomes. Although numerous forms stress-responsive alternative have been identified in model plants, a large-scale study dynamics under abiotic stress conditions cassava has not conducted. Here, we report the parallel employment isoform-Seq, ssRNA-Seq, Degradome-Seq to investigate diversity, abundance, fate alternatively spliced isoforms response...

Asphalt pavement cracks easily in low-temperature environments. In order to address the problem, phase-change material (PCM) microcapsules working at 0–10°C are used replace some of aggregates asphalt. this study, PCM with a high-temperature epoxy resin adhesive as reinforcement phase were prepared by using n-tetradecane/tetradecanol core and melamine-urea-formaldehyde wall material. The reinforced blended into asphalt modified styrene-butadiene-styrene block copolymer (SBS) asphalt, part...

DNA double-strand break (DSB) formation is the initial event for meiotic recombination catalyzed by conserved Spo11 protein. In Arabidopsis, several proteins have been reported to be involved in DSB formation. Here, we report an Arabidopsis forming (DFO) gene that The dfo mutant exhibits reduced fertility, producing polyads with abnormal number of microspores, unlike tetrads wild type. meiocytes were defective homologous chromosome synapsis and segregation. Genetic analysis revealed Atdfo-1...

Abiotic stress negatively impacts cassava (Manihot esculenta) growth and yield. Several molecular mechanisms of plant response to cold drought have been identified described in the literature, however, little is known about crosstalk responses these two stresses. To elucidate this question, transcriptome analysis seedlings under or PEG-simulated treatment was performed. Our results showed that 6103 7462 transcripts were significantly regulated by stress, respectively. Gene Ontology...

Meiotic recombination is required for proper homologous chromosome segregation in plants and other eukaryotes. The eukaryotic RAD51 gene family has seven ancient paralogs with important roles mitotic meiotic recombination. Mutations mammalian homologs RAD51C XRCC3 lead to embryonic lethality. In the model plant Arabidopsis thaliana, are not essential vegetative development but each somatic recombination, mechanism of unclear. non-lethal rad51c xrcc3 null mutants provide an opportunity study...

Long noncoding RNAs (lncRNAs) have been considered to be important regulators of gene expression in a range biological processes plants. A large number lncRNAs identified However, most their functions still remain determined. Here, we total 3004 cassava under normal or cold-treated conditions from Iso-seq data. We further characterized cold-responsive intergenic lncRNA 1 (CRIR1) as novel positive regulator the plant response cold stress. CRIR1 can significantly induced by treatment. Ectopic...

During meiotic recombination, induced double-strand breaks (DSBs) are processed into crossovers (COs) and non-COs (NCO); the former required for proper chromosome segregation fertility. DNA synthesis is essential in current models of recombination pathways includes only leading strand synthesis, but few genes crucial have been tested genetically their functions meiosis. Furthermore, lagging has assumed to be unnecessary. Here we show that Arabidopsis thaliana REPLICATION FACTOR C1 (RFC1)...

Summary The eukaryotic RAD 51 gene family has seven ancient paralogs conserved between plants and animals. Among these, , DMC 1 C XRCC 3 are important for homologous recombination and/or DNA repair, whereas single mutants in B D or 2 show normal meiosis, the lineages they represent diverged from each other evolutionarily later than four paralogs, suggesting possible functional redundancy. function of A rabidopsis genes mitotic repair meiosis was analyzed using molecular genetic, cytological...

Abstract Ferroelectric polarization switching and its domain evolution play a key role on the macroscopic electric properties of ferroelectric or piezoelectric devices. Mechanical has been reported recently in ~5 nm BaTiO 3 PbZr 0.2 Ti 0.8 O epitaxial films; however it is still challenge for mechanical force to switch slightly thicker film same way as an field. Here, we report that 70 BiFeO can be completely switched by similar observed with electrical switching. With gradual increase...

Abstract Polyphenols based bioactive compounds from vegetables and fruits are known for impressive antioxidant activity. Ingestion of these antioxidants may promote human health against cardiovascular diseases cancer. Mango is a popular tropical fruit with special taste, high nutritional value health-enhancing metabolites. The aim was to investigate the diversity phytochemicals between two mango cultivars china at three stages maturity. We used ESI-QTRAP-MS/MS approach characterize...

Abstract The harm and treatment of methyl blue (MB) were described, the necessity for removal MB by magnetic nanomaterials was explained. Magnetic Ni–Mg–Co ferrites prepared rapid combustion approach an active nitrate solution characterized X‐ray diffraction (XRD), scanning electron microscope (SEM), Fourier transform infrared spectrometer (FTIR), energy dispersive spectroscopy, vibrating sample magnetometer. Ni 0.1 Mg 0.7 Co 0.2 Fe 2 O 4 nanoparticles at 400°C with saturation magnetization...

MYB transcription factors (TFs) make up one of the most important TF families in plants. These proteins play crucial roles processes related to development, metabolism, and stimulus-response; however, very few studies have been reported for characterization TFs from banana. The current study identified 305 251 genes Musa acuminata balbisiana, respectively. Comprehensive details MYBs are terms gene structure, protein domain, chromosomal localization, phylogeny, expression patterns. Based on...

The exploration of catalysts for the oxygen evolution reaction (OER) with high activity and acceptable price is essential water splitting to hydrogen generation. High-entropy materials (HEMs) have aroused increasing interest in field electrocatalysis due their unusual physicochemical properties. In this work, we reported a novel FeCoNiMoZn-OH entropy hydroxide (HEH)/nickel foam (NF) synthesized by facile pulsed electrochemical deposition method at room temperature. HEH displays 3D porous...

Meiosis halves diploid genomes to haploid and is essential for sexual reproduction in eukaryotes. Meiotic recombination ensures physical association of homologs their subsequent accurate segregation results the redistribution genetic variations among progeny. Most organisms have two classes cross-overs (COs): interference-sensitive (type I) -insensitive II) COs. DNA synthesis meiotic recombination, but whether has a role differentiating CO pathways unknown. Here, we show that Arabidopsis...

Bananas (Musa spp.) are the most important fruit crops worldwide due to their high nutrition value. Fusarium wilt of banana, caused by fungal pathogen oxysporum f. sp. cubense tropical race 4 (Foc 4), is considered as destructive disease in world and results extensive damage leading productivity loss. The widespread use plant resistance inducers (PRIs), such benzothiadiazole (BTH), a novel strategy stimulate defense responses banana plants protect against pathogens infection. recent focus on...

Avocado (Persea americana Mill.) is an economically important crop because of its high nutritional value. However, the absence a sequenced avocado reference genome has hindered investigations secondary metabolism. For next-generation high-throughput transcriptome sequencing, we obtained 365,615,152 and 348,623,402 clean reads as well 109.13 104.10 Gb sequencing data for mesocarp seed, respectively, during five developmental stages. High-quality were assembled into 100,837 unigenes with...

Abstract A recent breakthrough in mechanical polarization switching provides a valuable handle to achieve nanoscale ferroelectric domain control. This flexoelectric is usually observed ultrathin films (≈10 nm or less thickness), where large strain gradient possible. However, from the point of view device applications, it will be more attractive thicker films. Here, experimentally demonstrated that by introducing ferroelastic ‐domains PbZr 0.1 Ti 0.9 O 3 potential barrier against 180° c...