A5029328883- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Neuroendocrine regulation and behavior
- Neuroscience and Neural Engineering
- Neuroscience of respiration and sleep
- Receptor Mechanisms and Signaling
- Memory and Neural Mechanisms
- Stress Responses and Cortisol
- Photochromic and Fluorescence Chemistry
- Infant Health and Development
- Advanced Fluorescence Microscopy Techniques
- Sleep and Wakefulness Research
- Neonatal Respiratory Health Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Zebrafish Biomedical Research Applications
- Neurobiology and Insect Physiology Research
- Circadian rhythm and melatonin
- Advanced Memory and Neural Computing
- Protein Kinase Regulation and GTPase Signaling
- CRISPR and Genetic Engineering
- 14-3-3 protein interactions
- Neuroscience, Education and Cognitive Function
- Cell Image Analysis Techniques
- Force Microscopy Techniques and Applications
Johns Hopkins Medicine
2020-2025
Johns Hopkins University
2020-2025
Max Planck Florida Institute for Neuroscience
2016-2024
Max Planck Institute of Neurobiology
2018-2023
H. L. Snyder Medical Foundation
2023
Korea University
2022
Albert Einstein College of Medicine
2021
Howard Hughes Medical Institute
2012-2016
Harvard University
2012-2016
Stress can be a motivational force for decisive action and adapting to novel environment; whereas, exposure chronic stress contributes the development of depression anxiety. However, molecular mechanisms underlying stress-responsive behaviors are not fully understood. Here, we identified orphan receptor GPR158 as regulator operating in prefrontal cortex (PFC) that links depression. is highly upregulated PFC human subjects with major depressive disorder. Exposure mice also increased protein...
Membrane nanotubes or tunneling (TNTs) that connect cells have been recognized as a previously unidentified pathway for intercellular transport between distant cells. However, it is unknown how this delicate structure, which extends over tens of micrometers and remains robust hours, formed. Here, we found TNT develops from double filopodial bridge (DFB) created by the physical contact two filopodia through helical deformation DFB. The transition DFB to close-ended most likely triggered...
Abstract Verifying causal effects of neural circuits is essential for proving a direct circuit-behavior relationship. However, techniques tagging only active neurons with high spatiotemporal precision remain at the beginning stages. Here we develop soma-targeted Cal-Light (ST-Cal-Light) which selectively converts somatic calcium rise triggered by action potentials into gene expression. Such modification simultaneously increases signal-to-noise ratio reporter expression and reduces light...
Cognitive flexibility is a brain's ability to switch between different rules or action plans depending on the context. However, cellular level understanding of cognitive have been largely unexplored. We probed specific serotonergic pathway from dorsal raphe nuclei (DRN) orbitofrontal cortex (OFC) while animals are performing reversal learning task. found that serotonin release DRN OFC promotes learning. A long-range connection these two brain regions was confirmed anatomically and...
Abstract Genetically defined subgroups of inhibitory interneurons are thought to play distinct roles in learning, but heterogeneity within these has limited our understanding the scope and nature their specific contributions. Here we reveal that chandelier cell (ChC), an interneuron type specializes inhibiting axon-initial segment (AIS) pyramidal neurons, establishes cortical microcircuits for organizing neural coding through selective axo-axonic synaptic plasticity. We found organized motor...
Abstract Oxytocin (OXT) is a neuropeptide originating in the paraventricular nucleus (PVN) of hypothalamus, with role influencing various social behaviors. However, pinpointing its actions only during time animals are performing specific behaviors has been difficult to study. Here we developed an optogenetic gene expression system designed selectively label neuronal populations activated by OXT presence blue-light, named “OXTR-iTango2”. The OXTR-iTango2 was capable inducing reporter both...
Neurotransmitter uncaging, especially that of glutamate, has been used to study synaptic function for over 30 years. One limitation caged glutamate probes is the blockade γ-aminobutyric acid (GABA)-A receptor function. This problem comes fore when are applied at high concentrations required effective two-photon photolysis. To mitigate such problems one could improve photochemical properties caging chromophores and/or remove blockade. We show addition a dicarboxylate unit widely...
IgSF11 homophilic cell adhesion molecules regulate the synaptic laminar specificity of cortical chandelier cells.
Protein kinases are key signaling nodes that regulate fundamental biological and disease processes. Illuminating kinase from multiple angles can provide deeper insights into mechanisms improve therapeutic targeting. While fluorescent biosensors powerful tools for visualizing live-cell activity dynamics in real time, new molecular needed enable recording of transient activities post hoc analysis targeted manipulation. Here, we develop a light-gated coupled transcriptional integrator (KINACT)...
Abstract Oxytocin is a neuropeptide important for maternal physiology and childcare, including parturition milk ejection during nursing 1–8 . Suckling triggers oxytocin release, but other sensory cues- specifically infant cries- can elevate levels in new human mothers 9–11 indicating that cries activate hypothalamic neurons. Here we describe neural circuit routing auditory information about vocalizations to mouse We performed vivo electrophysiological recordings photometry from identified...
Abstract Oxytocin is a neuropeptide important for maternal physiology and childcare, including parturition milk ejection during nursing1-8. Suckling triggers oxytocin release, but other sensory cues- specifically infant cries- can elevate levels in new human mothers9-11 indicating that cries activate hypothalamic neurons. Here we describe neural circuit routing auditory information about vocalizations to mouse We performed vivo electrophysiological recordings photometry from identified...
Dendritic protrusions, mainly spines and filopodia, correlate with excitatory synapses in pyramidal neurons (PyNs), but this relationship may not apply universally. We found that ectopic H-Ras expression increased protrusions across various cortical cell types, including layer 2/3 PyNs, parvalbumin (PV)-, vasoactive intestinal peptide (VIP)-positive interneurons (INs) the primary motor cortex. The probability of detecting correlated local activity, indicating its role protrusion formation....
Abstract Sleep and circadian rhythm disruption (SCRD) is commonly observed in aging, especially individuals who experience progressive cognitive decline to mild impairment (MCI) Alzheimer’s disease (AD). However, precise molecular mechanisms underlying the association between SCRD aging are not fully understood. Orexin A a well-characterized “sleep neuropeptide” that expressed hypothalamic neurons evokes wake behavior. The importance of exemplified narcolepsy where it profoundly...