A5100743505- Neuroscience and Neuropharmacology Research
- Cellular transport and secretion
- Photoreceptor and optogenetics research
- Genetics and Neurodevelopmental Disorders
- Stress Responses and Cortisol
- Neuroscience and Neural Engineering
- Neuroendocrine regulation and behavior
- Neurogenesis and neuroplasticity mechanisms
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Protein Kinase Regulation and GTPase Signaling
- Advanced Photocatalysis Techniques
- Catalytic Processes in Materials Science
- Protein Tyrosine Phosphatases
- Neurobiology and Insect Physiology Research
- CRISPR and Genetic Engineering
- Electrocatalysts for Energy Conversion
- Neural dynamics and brain function
- Advanced Electron Microscopy Techniques and Applications
- Catalysts for Methane Reforming
- Receptor Mechanisms and Signaling
- Memory and Neural Mechanisms
- Force Microscopy Techniques and Applications
- Infections and bacterial resistance
- Diamond and Carbon-based Materials Research
Hanyang University
2024
Korea University
2013-2023
Korea Electronics Technology Institute
2023
Sogang University
2014-2023
Johns Hopkins University
2022
Chonnam National University
2019-2020
Kookmin University
2015-2019
Max Planck Florida Institute for Neuroscience
2017
Korea Institute of Energy Research
2014
Sunchon National University
2013
Shank3 is a postsynaptic scaffolding protein implicated in synapse development and autism spectrum disorders. The gene known to produce diverse splice variants whose functions have not been fully explored. In the present study, we generated mice lacking exon 9 (Shank3 (Δ9) mice), thus missing five out of 10 containing N-terminal ankyrin repeat region, including longest variant, Shank3a. Our X-gal staining results revealed that proteins encoded by 9-containing are abundant upper cortical...
The MAM domain-containing GPI anchor proteins MDGA1 and MDGA2 are Ig superfamily adhesion molecules composed of six IG domains, a fibronectin III domain, anchor. MDGAs contribute to the radial migration positioning subset cortical neurons during early neural development. However, continue be expressed in postnatal brain, their functions development remain unknown. Here, we demonstrate that specifically with nanomolar affinity bind neuroligin-2, cell-adhesion molecule inhibitory synapses, but...
Significance This paper documents and systematically characterizes the molecular interactions of protein tyrosine phosphatase σ (PTPσ) with glypicans (GPCs). The identified require heparan sulfate (HS), suggesting that GPCs are a major source HS for PTPσ at excitatory synapses. Strikingly, we found leucine-rich repeat transmembrane 4 (LRRTM4) induces presynaptic differentiation via PTPσ/GPC interaction, may function as coreceptor in neurons. More importantly, HS-binding ability is critical...
Synaptic adhesion molecules regulate diverse aspects of synapse formation and maintenance. Many known synaptic localize at excitatory synapses, whereas relatively little is about inhibitory molecules. Here we report that IgSF9b a novel, brain-specific, homophilic molecule strongly expressed in GABAergic interneurons. was preferentially localized synapses cultured rat hippocampal cortical interneurons required for the development onto formed subsynaptic domain distinct from GABAA receptor–...
Multiple synaptic adhesion molecules govern synapse formation. Here, we propose calsyntenin-3/alcadein-β as a organizer that specifically induces presynaptic differentiation in heterologous synapse-formation assays. Calsyntenin-3 (CST-3) is highly expressed during various postnatal periods of mouse brain development. The simultaneous knockdown all three CSTs, but not CST-3 alone, decreases inhibitory, excitatory, densities cultured hippocampal neurons. Moreover, the CSTs reduces inhibitory...
The four members of the LRRTM family (LRRTM1-4) are postsynaptic adhesion molecules essential for excitatory synapse development. They have also been implicated in neuropsychiatric diseases. Here, we focus on LRRTM3, showing that two distinct LRRTM3 variants generated by alternative splicing regulate interaction with PSD-95, but not its synapse-promoting activity. Overexpression either variant increased density dentate gyrus (DG) granule neurons, whereas knockdown decreased it. controlled...
Abstract Verifying causal effects of neural circuits is essential for proving a direct circuit-behavior relationship. However, techniques tagging only active neurons with high spatiotemporal precision remain at the beginning stages. Here we develop soma-targeted Cal-Light (ST-Cal-Light) which selectively converts somatic calcium rise triggered by action potentials into gene expression. Such modification simultaneously increases signal-to-noise ratio reporter expression and reduces light...
Abstract Stress management is necessary for vertebrate survival. Chronic stress drives depression by excitation of the lateral habenula (LHb), which silences dopaminergic neurons in ventral tegmental area (VTA) via GABAergic neuronal projection from rostromedial nucleus (RMTg). However, effect acute on this LHb-RMTg-VTA pathway not clearly understood. Here, we used fluorescent situ hybridisation and vivo electrophysiology mice to show that LHb aromatic l -amino acid decarboxylase-expressing...
Abstract Synaptic adhesion molecules regulate various aspects of synapse development, function and plasticity. These functions mainly involve trans -synaptic interactions positive regulations, whereas cis -interactions negative regulation are less understood. Here we report that SALM4, a member the SALM/Lrfn family synaptic molecules, suppresses excitatory development through inhibition SALM3, another SALM protein with synaptogenic activity. Salm4 -mutant ( −/− ) mice show increased numbers...
The homeostatic regulation of neuronal activity in glutamatergic and GABAergic synapses is critical for neural circuit development synaptic plasticity. induced expression the transcription factor early growth response 1 (Egr-1) neurons tightly associated with many forms activity, but underlying target genes brain remained to be elucidated. This study uses a quantitative real-time PCR approach, combination vivo chromatin immunoprecipitation, reveals that GABAA receptor subunit, GABRA2 (α2),...
Abstract Oxytocin (OXT) is a neuropeptide originating in the paraventricular nucleus (PVN) of hypothalamus, with role influencing various social behaviors. However, pinpointing its actions only during time animals are performing specific behaviors has been difficult to study. Here we developed an optogenetic gene expression system designed selectively label neuronal populations activated by OXT presence blue-light, named “OXTR-iTango2”. The OXTR-iTango2 was capable inducing reporter both...
Abstract Protein ubiquitination has a significant influence on diverse aspects of neuronal development and function. Dorfin, also known as Rnf19a, is RING finger E3 ubiquitin ligase implicated in amyotrophic lateral sclerosis Parkinson’s disease, but its vivo functions have not been explored. We report here that Dorfin novel binding partner the excitatory postsynaptic scaffolding protein PSD-95. Dorfin-mutant ( −/− ) mice show reduced adult neurogenesis enhanced long-term potentiation...
The recent advances in super-resolution fluorescence microscopy, including single-molecule localization microscopy (SMLM), has enabled the study of previously inaccessible details, such as organization proteins within cellular compartments and even nanostructures nonbiological nanomaterials, polymers semiconductors. With developments, need for development various computational nanostructure analysis methods SMLM images is also increasing; however, this been limited to protein cluster...
Abstract Inositol 1,4,5-trisphosphate 3-kinase A (IP 3 K-A) is a molecule enriched in the brain and neurons that regulates intracellular calcium levels via signaling through inositol trisphosphate receptor. In present study, we found IP K-A expression highly central nucleus of amygdala (CeA), which plays pivotal role processing emotional phenotypes mammals. Genetic abrogation altered gene expression, particularly genes involved key pathways mediating fear- anxiety-related behaviors....
Here, we report the generation and comprehensive characterization of a knockin mouse model for hotspot p.Arg87Cys variant cytoplasmic FMR1‐interacting protein 2 ( CYFIP2 ) gene, which was recently identified in individuals diagnosed with West syndrome, developmental epileptic encephalopathy. The Cyfip2 +/R87C mice recapitulated many neurological neurobehavioral phenotypes patients, including spasmlike movements, microcephaly, impaired social communication. Age‐progressive cytoarchitectural...
A 24-year-old man presented with mental change, fever, abdominal pain, tenderness and palpable mass on the lower abdomen. He was a non-Korean engineer did not accompany legal guardian, so medical history taking difficult due to his status. Brain magnetic resonance imaging showed multiple rim-enhanced lesions of brain, computed tomography huge paraspinal abscess. Chest X-ray poorly defined nodular opacities. We initially thought that this patient infected toxoplasmosis typical cerebral image...