A5011027783- Neuroscience and Neuropharmacology Research
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Neuroendocrine regulation and behavior
- Axon Guidance and Neuronal Signaling
- Mast cells and histamine
- Cellular transport and secretion
- Signaling Pathways in Disease
- Retinal Development and Disorders
- Congenital gastrointestinal and neural anomalies
- Research in Social Sciences
- Neuroinflammation and Neurodegeneration Mechanisms
- Pain Mechanisms and Treatments
- Olfactory and Sensory Function Studies
- Nicotinic Acetylcholine Receptors Study
- Congenital heart defects research
- Patient Safety and Medication Errors
- Ion Transport and Channel Regulation
- Pancreatic function and diabetes
- Pharmaceutical Practices and Patient Outcomes
- Hearing, Cochlea, Tinnitus, Genetics
- Sleep and Wakefulness Research
University of Helsinki
2009-2020
Finnish Brain Research and Rehabilitation Center Neuron
2007
Yale University
2006
Biocenter Finland
1999-2004
Czech Academy of Sciences, Institute of Biotechnology
1999-2002
Helsinki Institute of Physics
1999-2002
Wallenberg Wood Science Center
2000
Lund University
1999
Max Planck Institute of Neurobiology
1999
Max Planck Institute of Psychiatry
1998
Pathophysiological activity and various kinds of traumatic insults are known to have deleterious long-term effects on neuronal Cl- regulation, which can lead a suppression fast postsynaptic GABAergic responses. Brain-derived neurotrophic factor (BDNF) increases excitability through conjunction mechanisms that include regulation the efficacy transmission. Here, we show exposure rat hippocampal slice cultures acute slices exogenous BDNF or neurotrophin-4 produces TrkB-mediated fall in...
Intracellular calcium-binding proteins are abundantly expressed in many neuronal populations. Previous evidence suggests that can modulate various properties, presumably by their action as calcium buffers. The importance of for nervous system function an intact integrated is, however, less clear. To investigate the physiological role a major endogenous protein, calbindin D28k (calbindin) vivo , we have generated null mutant mice gene targeting. Surprisingly, deficiency does not affect...
Abstract Using an antiserum against conjugated histamine we mapped the histaminergic somata and their fiber projection areas in carbodiimide‐fixed guinea pig central nervous system. The neurons were large they found exclusively posterior hypothalamus, as rat, but more numerous distributed widely a thin layer around mammillary nucleus, scattered between within medial nuclei, dense cell cluster emerging from caudal magnocellular nucleus extending to preoptic area. density of...
Abstract Intracellular Cl – plays a key role in cellular volume regulation, cell cycle control and shaping the polarity of inhibitory postsynaptic responses mediated by anion‐permeable GABA glycine receptors. In this study, we have investigated expression patterns members cation‐chloride cotransporters (CCCs), including K‐Cl KCC1‐4 Na‐K‐2 cotranporter NKCC1 during rodent embryonic brain development. At time neurogenesis (embryonic days; E12.5–14.5), KCC1 was only detectable developing...
The object of this study was to develop an immunohistochemical method that could be used neuronal histamine, especially in nerve fibers and terminals where most previous methods have not been applicable. Three new antisera were produced rabbits against conjugated the fixative conjugation, 1-ethyl-3(3-diamethylaminopropyl)-carbodiimide (EDCDI), tissue fixation compared paraformaldehyde. Specificity established with dot-blot tests on nitrocellulose, blocking controls affinity-purified...
Purkinje cells are among the most resistant neurons to axotomy and refractory axonal regeneration. By using organotypic cultures, we have studied age- environment-related factors implicated in cell survival Most taken from 1- 5-d-old rats, period which these engaged intense synaptogenesis dendritic remodeling, die 1 week after plating, whereas if cultured before or this period, survive, even absence of deep nuclear neurons, their postsynaptic targets. Cerebellar slices 10-d-old rats kept...
Abstract Tuberomammillary neurons in the posterior hypothalamus are sole source of neuronal histamine adult mammalian brain. In rat, these cells reported to contain immunoreactivity for γM‐aminobutyric acid (GABA) and several neuropeptides. We compared presence substances tuberomammillary mouse, guinea pig. all three species, histamine‐immunoreactive cell bodies were positive GABA. This suggests that GABAergic transmission may be important function. No immunoreactive thyrotropin releasing...
Hyperpolarizing fast inhibitory neurotransmission by gamma-aminobutyric acid and glycine requires an efficient chloride extrusion mechanism in postsynaptic neurons. A major effector of this task adult animals is the potassium-chloride co-transporter KCC2 that selectively abundantly expressed postsynaptically most CNS Yet, role brain at systems level poorly known. Here, we characterize behaviour mice doubly heterozygous for null hypomorphic alleles retain 15-20% normal protein levels brain....
A major event in the maturation of CNS GABAergic transmission is qualitative change GABA -mediated responses from depolarizing to hyperpolarizing. In cortical regions, this attributed increased expression potassium chloride cotransporter 2b (KCC2b), main isoform neuron-specific K-Cl KCC2. We have previously shown that transcription factor early growth response 4 (Egr4) can activate KCC2b promoter. Here we demonstrate immature hippocampal neurons BDNF robustly induces ERK1/2 (extracellular...
The neuron-specific K-Cl cotransporter KCC2 maintains the low intracellular chloride concentration required for fast hyperpolarizing actions of inhibitory neurotransmitters. gene codes two isoforms, KCC2a and KCC2b, which differ in their N termini. relative expression cellular distribution protein isoforms are unknown. Here, we characterize an antibody against isoform show that a previously described is specific KCC2b (Hubner, C. A., Stein, V., Hermans-Borgmeyer, I., Meyer, T., Ballanyi, K.,...