A5062244268- Neuroscience and Neuropharmacology Research
- Nerve injury and regeneration
- Signaling Pathways in Disease
- Ion channel regulation and function
- RNA Interference and Gene Delivery
- Neurogenesis and neuroplasticity mechanisms
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- Receptor Mechanisms and Signaling
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Chromatin Remodeling and Cancer
- RNA modifications and cancer
- Congenital heart defects research
- RNA and protein synthesis mechanisms
- Hedgehog Signaling Pathway Studies
- Cannabis and Cannabinoid Research
- Neuroendocrine regulation and behavior
- Ion Transport and Channel Regulation
- Hemispheric Asymmetry in Neuroscience
- GABA and Rice Research
- Viral Infectious Diseases and Gene Expression in Insects
- Lysosomal Storage Disorders Research
- Viral Infections and Outbreaks Research
Heidelberg University
2017-2023
Tallinn University of Technology
2005-2017
National Institute of Chemical Physics and Biophysics
2004-2005
Estonian Biocentre
2004
Brain-derived neurotrophic factor (BDNF), a member of the nerve growth family neurotrophins, has central roles in development, physiology, and pathology nervous system. We have elucidated structure human BDNF gene, identified alternative transcripts, studied their expression adult tissues brain regions. In addition, transcription initiation sites for transcripts were determined activities promoters analyzed transient overexpression assays. Our results show that gene 11 exons nine functional...
A global concern has emerged with the pandemic spread of Zika virus (ZIKV) infections that can cause severe neurological symptoms in adults and newborns. ZIKV is a positive-strand RNA replicating virus-induced membranous replication factories (RFs). Here we used various imaging techniques to investigate ultrastructural details RFs their relationship host cell organelles. Analyses human hepatic cells neural progenitor infected revealed endoplasmic reticulum (ER) membrane invaginations...
Brain-derived neurotrophic factor (BDNF) is an important mediator of activity-dependent functions the nervous system and its expression dysregulated in several neuropsychiatric disorders. Regulation rodent BDNF neuronal transcription has been relatively well characterized. Here, we have studied regulation human (h ) by membrane depolarization cultured mouse or rat primary cortical neurons expressing h gene transfected with promoter constructs, respectively. We identified asymmetric...
The CB1 cannabinoid receptor, the main molecular target of endocannabinoids and cannabis active components, is most abundant G protein-coupled receptor in mammalian brain. In particular, highly expressed basal ganglia, mostly on terminals medium-sized spiny neurons, where it plays a key neuromodulatory function. also confers neuroprotection various experimental models striatal damage. However, assessment physiological relevance therapeutic potential ganglia-related diseases hampered, at...
Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, regulates both survival and differentiation several neuronal populations in nervous system during development, as well synaptic plasticity adult brain. BDNF exerts its biological functions through receptor TrkB. Although regulation transcription by activity has been widely studied, little is known about TrkB signaling-dependent expression . Using rat primary cortical neuron cultures, we show that gene subject to...
Transcription factor TCF4 (alias ITF2, SEF2 or E2-2) is a broadly expressed basic helix-loop-helix (bHLH) protein that functions as homo- heterodimer. Missense, nonsense, frame-shift and splice-site mutations well translocations large deletions encompassing gene cause Pitt-Hopkins syndrome (PTHS), rare developmental disorder characterized by severe motor mental retardation, typical facial features breathing anomalies. Irrespective of the mutation, haploinsufficiency has been proposed an...
Long-term adaptive responses in the brain, such as learning and memory, require synaptic activity-regulated gene expression, which has been thoroughly investigated rodents. Using human iPSC-derived neuronal networks, we show that mouse activity-induced transcriptional programs share many genes both Ca2+-regulated synapse-to-nucleus signaling. Species-specific differences include noncoding RNA BRE-AS1 LINC00473 protein-coding ZNF331, are absent genome, well several whose orthologs either not...
Four members of the nuclear factor activated T cells (NFAT) family (NFATC1, NFATC2, NFATC3, and NFATC4) are Ca2+-regulated transcription factors that regulate several processes in vertebrates, including development function immune, cardiovascular, musculoskeletal, nervous systems. Here we describe structures alternative splicing human mouse NFAT genes, novel splice variants for NFATC1, NFATC4, show expression different mRNAs various tissues brain regions by RT-PCR. Our results alternatively...
During cortical development, when NR2B subunit is the major component of NMDA glutamate receptors (NMDARs), moderate NMDAR activity supports neuronal survival at least in part by regulating gene transcription. We report that, cultured neurons from newborn rats, NMDARs activated calcium-responsive transcription regulator nuclear factor T cells (NFAT). Moreover, developing rat cortex, NFAT isoforms c3 and c4 (NFATc3 NFATc4) were expressed relatively higher levels postnatal day 7 (P7) than P21,...
Abstract X chromosome‐linked inhibitor of apoptosis protein (XIAP) is an anti‐apoptotic enhancing cell survival. Brain‐derived neurotrophic factor (BDNF) also promotes neuronal viability but the links between XIAP and BDNF have remained unclear. We show here that overexpression increases in transgenic mice cultured rat hippocampal neurons, whereas downregulation by silencing RNA decreased BDNF. stimulated signaling, as shown increased phosphorylation TrkB receptor downstream molecule, cAMP...
Transcription factor 4 (TCF4 also known as ITF2 or E2-2) is a basic helix-loop-helix (bHLH) protein associated with Pitt–Hopkins syndrome, intellectual disability, and schizophrenia (SCZ). Here, we show that TCF4-dependent transcription in cortical neurons cultured from embryonic rats of both sexes induced by neuronal activity via soluble adenylyl cyclase kinase A (PKA) signaling. PKA phosphorylates TCF4 directly phosphorylation site necessary for its transcriptional the developing brain...
Article18 October 2021Open Access Transparent process Npas4 regulates medium spiny neuron physiology and gates cocaine-induced hyperlocomotion Thomas Lissek Corresponding Author [email protected] orcid.org/0000-0003-4748-5474 Interdisciplinary Center for Neurosciences, Department of Neurobiology, Heidelberg University, Heidelberg, Germany Search more papers by this author Andry Andrianarivelo orcid.org/0000-0002-6369-4201 INSERM, UMR-S 1130, Neuroscience Paris Seine, Institute Biology Paris,...
Abstract Nuclear factor of activated T‐cells ( NFAT ) is a family transcription factors comprising four calcium‐regulated members: c1, c2, c3, and c4. Upon activation by the calcium‐dependent phosphatase calcineurin (CaN), s translocate from cytosol to nucleus regulate their target genes, which in nervous system are involved axon growth, synaptic plasticity, neuronal survival. We have shown previously that there number different splice variants genes expressed brain. Here, we studied...
Abstract The K + /Cl – co‐transporter KCC2 maintains the low intracellular chloride concentration required for fast synaptic inhibition and is exclusively expressed in neurones of CNS. Here, we show that gene (alias SLC12a5) has multiple transcription start sites characterize activity 6.8 kb mouse regulatory sequence (spanning 1.4 upstream from exon 1 to 2) using luciferase reporters. Overexpression neurone‐restrictive silencer factor repressed reporter vitro , apparently via a neurone...
Abstract Background Brain-derived neurotrophic factor (BDNF) is a small secreted protein that has important roles in the developing and adult nervous system. Altered expression or changes regulation of BDNF gene have been implicated variety human system disorders. Although rodent extensively investigated, vivo studies regarding are largely limited to postmortem analysis. Bacterial artificial chromosome (BAC) transgenic mice harboring its regulatory flanking sequences constitute useful tool...
Summary Bacteria use two‐component signal transduction pathways to sense both extracellular and intracellular environment coordinate cellular events according changing conditions. Adaptation can be either physiological or genetical. Here, we present evidence that a genome reorganization process such as transposition controlled by certain environmental cues sensed system. We demonstrate transposition‐dependent accumulation of phenol‐utilizing mutants is severely decreased in Pseudomonas...
J. Neurochem . (2009) 109 , 807–818. Abstract The SWItch/Sucrose NonFermentable, a nucleosome remodeling complex (SWI/SNF) chromatin‐remodelling complexes act upon the nucleosomal structure and regulate transcription, replication, repair of chromatin splicing. In this study, we present evidence that human, mouse rat genes encoding one SWI/SNF subunits, BAF57, undergo neuron‐specific splicing exons II, III IV. Alternative yields in at least three isoforms BAF57 protein have truncated...
KCNIP3/KChIP3 (voltage-dependent K+ channel interacting protein 3), alias Calsenilin and downstream regulatory element antagonist modulator (DREAM), is a multifunctional that modulates A-type potassium channels, affects processing of amyloid precursor regulates transcription. KCNIP3 has been described to negatively influence the activity CREB (cAMP/Ca(2+)-response binding protein), an essential factor in neuronal activity-dependent gene expression regulation. However, reports on...