A5063696712- SARS-CoV-2 and COVID-19 Research
- Neurobiology and Insect Physiology Research
- Cellular transport and secretion
- Genetics, Aging, and Longevity in Model Organisms
- Olfactory and Sensory Function Studies
- CRISPR and Genetic Engineering
- Axon Guidance and Neuronal Signaling
- Photosynthetic Processes and Mechanisms
- Neuroendocrine regulation and behavior
- Virus-based gene therapy research
- Nerve injury and regeneration
- Viral Infectious Diseases and Gene Expression in Insects
- COVID-19 Clinical Research Studies
- RNA Interference and Gene Delivery
- Retinal Development and Disorders
- Neuropeptides and Animal Physiology
- Monoclonal and Polyclonal Antibodies Research
- Physiological and biochemical adaptations
- Neuroscience and Neuropharmacology Research
- Wnt/β-catenin signaling in development and cancer
- Renal and related cancers
- Immune responses and vaccinations
- Reproductive Biology and Fertility
- Protein purification and stability
- Congenital gastrointestinal and neural anomalies
Genetika
2017-2024
University of Basel
2007-2014
Helsinki Institute of Physics
2008
University of Helsinki
1999-2008
Friedrich Miescher Laboratory
2005
Max Planck Society
2005
Biocenter Finland
1999-2003
Engelhardt Institute of Molecular Biology
1996-2001
Glial cell line‐derived neurotrophic factor (GDNF) has potentially great clinical importance in the treatment of Parkinson's disease and several other neurodegenerative diseases, however its intracellular signaling mechanisms are poorly understood. Here we show that upon GDNF binding glycosyl‐phosphatidylinositol (GPI)‐linked receptor α1 (GFRα1) activates cytoplasmic Src family tyrosine kinase(s) Ret kinase‐deficient cultured mouse dorsal root ganglion neurons two Ret‐negative lines....
The endoplasmic reticulum (ER) is the major intracellular membrane system. ER essential for protein and lipid biosynthesis, transport of proteins along secretory pathway, calcium storage. Here, we describe our investigations into dynamics regulation in early Caenorhabditis elegans embryo. Using a GFP fusion to ER-resident signal peptidase SP12, observed morphological transitions through fertilization cell-cycles living embryos. These were tightly coordinated with division cycle: upon onset...
In many organisms, cortical granules undergo exocytosis following fertilization, releasing cargo proteins that modify the extracellular covering of zygote. We identified in Caenorhabditis elegans and have found degranulation occurs a wave initiates vicinity meiotic spindle during anaphase I. Previous studies genes confer an embryonic osmotic sensitivity phenotype,thought to result from abnormal eggshell formation. Many these are components cell cycle machinery. When we suppressed expression...
Glial cell line-derived neurotrophic factor (GDNF) family ligands signal through receptor complex consisting of a glycosylphosphatidylinositol-linked GDNF (GFR) α subunit and the transmembrane tyrosine kinase RET. The inherited cancer syndrome multiple endocrine neoplasia type 2 (MEN2), associated with different mutations in RET, is characterized by medullary thyroid carcinoma. signals via GFRα1, neurturin GFRα2, artemin GFRα3, whereas mammalian GFRα for persephin (PSPN) unknown. Here we...
Glial cell line–derived neurotrophic factor (GDNF) and hepatocyte growth (HGF) are multifunctional signaling molecules in embryogenesis. HGF binds to activates Met receptor tyrosine kinase. The complex for GDNF typically includes both family α1 (GFRα1) Ret can also signal independently of via GFRα1, although the mechanism has remained unclear. We now show that partially restores ureteric branching morphogenesis ret-deficient mice with severe renal hypodysplasia. Ret-independent effect was...
With the onset of COVID-19 pandemic, a number molecular-based tests have been developed to diagnose SARS-CoV-2 infection. However, numerous available serological lack sufficient sensitivity or specificity. They do not detect specific antibodies in significant proportion patients with PCR-confirmed COVID-19. There is evidence that some convalescents relatively short-lived humoral immunity. In contrast, publications shown T-cell response human coronaviruses, including SARS-CoV-1, MERS, and...
Caenorhabditis elegans has recently been used as an attractive model system to gain insight into mechanisms of endocytosis in multicellular organisms. A combination forward and reverse genetics identified a number new membrane trafficking factors. Most them have mammalian homologues which function the same transport events. We describe novel C. gene sand-1, whose loss causes profound endocytic defects many tissues. SAND-1 belongs conserved family proteins present all eukaryotic species,...
INTRODICTION . Currently, gene therapy based on adeno-associated virus (AAV) vectors faces a number of barriers, both biomedical and technological, which require studying overcoming for further development this technology. AIM This study aimed to analyse the use range hereditary diseases, taking into account barriers associated with its side effects insufficient efficacy, determination therapeutic window, individual characteristics relevant particular disease; additionally, review approaches...
Determination of T-cell immune response to SARS-CoV-2 is important both for diagnosis the disease in symptomatic patients, and determination total number people who have had disease, including its asymptomatic form. In addition, these assays are efficient assessing after vaccination, as well immunity levels risk groups convalescent patients. The most promising method assessment an ELISPOT-based assay measuring release interferon-gamma (IGRA) stimulation with coronavirus-specific antigens....
We report the isolation of a cDNA clone encoding neuropeptide precursor named preproGFAD from central nervous system (CNS) snail Helix lucorum. Analysis expression this gene shows that it is neurospecific and expressed in several groups CNS neurons. Most notable right mesocerebrum, where neurons controlling mating behavior are located. The particular region observed adult animals but not juvenile ones. preprohormone 108 amino acids long contains hydrophobic leader peptide eight Lys-Arg...
WE report the isolation of a Helix lucorum cDNA clone encoding precursor neuropeptides that are closely related to Aplysia and Tritonia pedal peptides (Pep). The predicted propeptide contains 20 copies two variants Pep interspersed with Lys-Arg endopeptidase cleavage sites. Northern blot hybridization revealed multiple Pep-hybridizing species in CNS RNA. gene was expressed by several identified serotonergic neurones cerebral ganglia, groups sensory procerebrum, peripheral olfactory bulb,...