A5065518722- Ion channel regulation and function
- Venomous Animal Envenomation and Studies
- Nicotinic Acetylcholine Receptors Study
- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Insect and Pesticide Research
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neuropharmacology Research
- Marine Toxins and Detection Methods
- Insect Resistance and Genetics
- Toxin Mechanisms and Immunotoxins
- Healthcare and Venom Research
- Chemical Synthesis and Analysis
- Beetle Biology and Toxicology Studies
- Protein Hydrolysis and Bioactive Peptides
- Immunodeficiency and Autoimmune Disorders
- Monoclonal and Polyclonal Antibodies Research
- Receptor Mechanisms and Signaling
- Extraction and Separation Processes
- Adenosine and Purinergic Signaling
- Plant-based Medicinal Research
- Neuropeptides and Animal Physiology
- Chemical Synthesis and Characterization
- Probiotics and Fermented Foods
- Force Microscopy Techniques and Applications
Institute of Bioorganic Chemistry
2016-2025
Moscow Institute of Physics and Technology
2018-2025
Russian Academy of Sciences
2008-2025
Institute of Evolutionary Physiology and Biochemistry
2025
Moscow Power Engineering Institute
2021-2024
Moscow Aviation Institute
2024
Institute of Physics and Technology
2018-2022
Lomonosov Moscow State University
2016-2021
Sechenov University
2020
International Medical Research (Germany)
2019
Seven novel short linear antimicrobial and cytolytic peptides named latarcins were purified from the venom of spider <i>Lachesana tarabaevi</i>. These found to produce lytic effects on cells diverse origin (Gram-positive Gram-negative bacteria, erythrocytes, yeast) at micromolar concentrations. In addition, five that share considerable structural similarity with predicted <i>L</i>. <i>tarabaevi</i> gland expressed sequence tag data base. Latarcins shown adopt amphipathic α-helical structure...
This study presents purification, activity characterization, and (1)H NMR of the novel antifungal peptide EcAMP1 from kernels barnyard grass Echinochloa crus-galli. The adopts a disulfide-stabilized α-helical hairpin structure in aqueous solution thus represents fold among naturally occurring antimicrobial peptides. Micromolar concentrations were shown to inhibit growth several fungal phytopathogens. Confocal microscopy revealed intensive binding surface conidia followed by internalization...
The multilayered plant immune system relies on rapid recognition of pathogen-associated molecular patterns followed by activation defense-related genes, resulting in the reinforcement cell walls and production antimicrobial compounds. To suppress defense, fungi secrete effectors, including a recently discovered Zn-metalloproteinase from Fusarium verticillioides, named fungalysin Fv-cmp. This proteinase cleaves class IV chitinases, which are defense proteins that bind degrade chitin fungal...
Apamin is often cited as one of the few substances selectively acting on small-conductance Ca2+-activated potassium channels (KCa2). However, published pharmacological and structural data remain controversial. Here, we investigated molecular pharmacology apamin by two-electrode voltage-clamp in Xenopus laevis oocytes patch-clamp HEK293, COS7, CHO cells expressing studied ion channels, well isolated rat brain neurons. The microtitre broth dilution method was used for antimicrobial activity...
Two forms of a novel antimicrobial peptide (AMP), named WAMP-1a and WAMP-1b, that differ by single C-terminal amino acid residue belong to new structural type plant AMP were purified from seeds Triticum kiharae Dorof. et Migusch. Although WAMP-1b share similarity with hevein-type peptides, they possess 10 cysteine residues arranged in unique motif which is distinct those described previously for AMPs, but characteristic the chitin-binding domains cereal class I chitinases. An unusual...
A new peptide trypsin inhibitor named BWI-2c was obtained from buckwheat (Fagopyrum esculentum) seeds by sequential affinity, ion exchange and reversed-phase chromatography. The sequenced found to contain 41 amino acid residues, with four cysteine residues involved in two intramolecular disulfide bonds. Recombinant identical the natural produced Escherichia coli a form of cleavable fusion thioredoxin. 3D (three-dimensional) structure solution determined NMR spectroscopy, revealing...
Kalium ( http://kaliumdb.org/ ) is a manually curated database that accumulates data on potassium channel toxins purified from scorpion venom (KTx). This an open-access resource, and provides easy access to pages of other databases interest, such as UniProt, PDB, NCBI Taxonomy Browser, PubMed. General achievements are strict regulation KTx classification based the unified nomenclature supported by researchers in field, removal peptides with partial sequence entries transcriptomic information...
Eight linear cationic peptides with cytolytic and insecticidal activity, designated cyto-insectotoxins (CITs), were identified in Lachesana tarabaevi spider venom. The showed antibiotic activity towards Gram-positive Gram-negative bacteria at micromolar concentrations as well toxicity to insects. primary structures of the toxins established by direct Edman sequencing combination enzymatic chemical polypeptide degradation MS. CITs represent a novel class molecules venom toxins. They are first...
P2X3 purinoreceptors expressed in mammalian sensory neurons play a key role several processes, including pain perception. From the venom of Central Asian spider Geolycosa sp., we have isolated novel peptide, named purotoxin-1 (PT1), which is to our knowledge first natural molecule exerting powerful and selective inhibitory action on receptors. PT1 dramatically slows down removal desensitization these The peptide demonstrates potent antinociceptive properties animal models inflammatory pain.
A novel family of antifungal peptides was discovered in the wheat T riticum kiharae Dorof. et Migusch. Two members family, designated Tk‐ AMP ‐ X 1 and k‐ ‐X2, were completely sequenced shown to belong α‐hairpinin structural plant with a characteristic C1XXXC2‐X(n)‐C3XXXC4 motif. The inhibit spore germination several fungal pathogens vitro . c DNA gene cloning disclosed unique structure genes encoding ‐X peptides. They code for precursor proteins unusual multimodular structure, consisting...