A5001449058- Ion channel regulation and function
- Nicotinic Acetylcholine Receptors Study
- Genomics and Chromatin Dynamics
- Cardiac electrophysiology and arrhythmias
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Venomous Animal Envenomation and Studies
- biodegradable polymer synthesis and properties
- Insect and Pesticide Research
- Electrospun Nanofibers in Biomedical Applications
- DNA Repair Mechanisms
- RNA Research and Splicing
- Bone Tissue Engineering Materials
- Force Microscopy Techniques and Applications
- Radiopharmaceutical Chemistry and Applications
- Antimicrobial Peptides and Activities
- 3D Printing in Biomedical Research
- Advanced biosensing and bioanalysis techniques
- Receptor Mechanisms and Signaling
- Bioactive Natural Diterpenoids Research
- Genetics, Aging, and Longevity in Model Organisms
- Insect Resistance and Genetics
- Microfluidic and Bio-sensing Technologies
- Neuroscience and Neuropharmacology Research
- Alkaloids: synthesis and pharmacology
Institute of Gene Biology
2024
Institute of Bioorganic Chemistry
2013-2021
Lomonosov Moscow State University
2011-2021
Russian Academy of Sciences
2013-2015
Moscow State University
2011-2013
A correct chromatin structure is important for cell viability and tightly regulated by numerous factors. Human protein complex FACT (facilitates transcription) an essential factor involved in transcription cancer development. Here FACT-dependent changes the of single nucleosomes were studied with single-particle Förster resonance energy transfer (spFRET) microscopy using labeled a donor-acceptor pair fluorophores, which attached to adjacent gyres DNA near contact between H2A-H2B dimers. its...
Abstract Ion channels play a central role in host of physiological and pathological processes are the second largest target for existing drugs. There is an increasing need reliable tools to detect visualize particular ion channels, but solutions suffer from number limitations such as high price, poor specificity, complicated protocols. As alternative, we produced recombinant chimeric constructs (FP-Tx) consisting fluorescent proteins (FP) fused with potassium channel toxins scorpion venom...
Abstract DNA accessibility to various protein complexes is essential for processes in the cell and affected by nucleosome structure dynamics. Protein factor PARP-1 (poly(ADP-ribose) polymerase 1) increases of chromatin repair proteins transcriptional machinery, but mechanism extent this reorganization are unknown. Here we report on effects single nucleosomes revealed spFRET (single-particle FÖrster Resonance Energy Transfer) microscopy. binding a double-strand break vicinity results...
Ether-a-go-go family (EAG) channels play a major role in many physiological processes humans, including cardiac repolarization and cell proliferation. Cryo-EM structures of two them, KV10.1 human ether-a-go-go-related gene (hERG or KV11.1), have revealed an original nondomain-swapped structure, suggesting that the mechanism voltage-dependent gating these is quite different from classical mechanical-lever model. Molecular aspects hERG voltage-gating been extensively studied, indicating S4-S5...
The aim of the investigation was to develop a technology manufacturing bone implants based on hybrid polymer construction composed poly(3-hydroxybutyrate) and sodium alginate for guided regeneration using 3D printing method.Materials Methods.Complex shaped were manufactured by method two-stage leaching mold obtained printing.The appearance, morphology structure scaffolds analyzed means scanning electron microscopy.Biocompatibility in vivo determined histology data implantation as...
Recently developed fluorescent protein-scorpion toxin chimeras (FP-Tx) show blocking activities for potassium voltage-gated channels of Kv1 family and retain almost fully pharmacological profiles the parental peptide toxins (Kuzmenkov et al., Sci Rep. 2016, 6, 33314). Here we report on N-terminally green protein (GFP)-tagged agitoxin 2 (GFP-L2-AgTx2) with high affinity selectivity binding site Kv1.3 channel involved in pathogenesis various (primarily autoimmune origin) diseases. The basis...
The antimicrobial peptide Ltc1-K and its derivates without one, two, then three N-terminal amino acid residues were studied based on the hypothesis (backed by some experimental data) that hydrophobic moiety of linear cationic peptides defines their haemolytic activity. It was discovered excision considerably decreases peptide's toxicity for eukaryotic cells simultaneously increases selectivity antibacterial activity bacteria species. Studies performed with model membrane systems human...