A5083522302- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Pain Mechanisms and Treatments
- Adenosine and Purinergic Signaling
- Ion Channels and Receptors
- Botulinum Toxin and Related Neurological Disorders
- Neuropeptides and Animal Physiology
- Biochemical Analysis and Sensing Techniques
- Neurobiology and Insect Physiology Research
- Nerve Injury and Rehabilitation
- Mitochondrial Function and Pathology
- Spinal Cord Injury Research
- Receptor Mechanisms and Signaling
- Biochemical effects in animals
- Cellular transport and secretion
- Salivary Gland Disorders and Functions
- Adipose Tissue and Metabolism
- Connexins and lens biology
- Pharmacological Effects of Natural Compounds
- Neuroscience of respiration and sleep
- Nicotinic Acetylcholine Receptors Study
- Endodontics and Root Canal Treatments
- Neurogenesis and neuroplasticity mechanisms
- Musculoskeletal pain and rehabilitation
- Spine and Intervertebral Disc Pathology
Kyiv Academic University
2017-2025
Bogomoletz Institute of Physiology
2013-2023
National Academy of Sciences of Ukraine
2002-2021
Institute of Molecular Biology and Genetics
2013
Moscow Institute of Physics and Technology
2012
Lviv University
2012
Johns Hopkins Medicine
2009
Johns Hopkins University
2009
Iowa State University
2004-2005
Spinal cord GluR2-lacking AMPA receptors (AMPARs) contribute to nociceptive hypersensitivity in persistent pain, but the molecular mechanisms underlying this event are not completely understood. We report that complete Freund's adjuvant (CFA)-induced peripheral inflammation induces synaptic GluR2 internalization dorsal horn neurons during maintenance of CFA-evoked hypersensitivity. This is initiated by phosphorylation at Ser 880 and subsequent disruption binding its anchoring protein (GRIP),...
ATP receptor mediated Ca2+ signaling was recorded from Bergmann glial cells in cerebellar slices obtained mice of different ages (postnatal days 6 to 45). To measure the cytoplasmic concentration ([Ca2+]in), either individual were loaded with Ca(2+)-sensitive probes using whole cell patch clamp technique or incubated dye and microfluorimetric system focused on cells. Signals single-detector microfluorimetry fura-2 by confocal laser scanning (fluo-3-based recordings). Extracellular...
Peripheral inflammation alters AMPA receptor (AMPAR) subunit trafficking and increases AMPAR Ca(2+) permeability at synapses of spinal dorsal horn neurons. However, it is unclear whether extrasynaptic sites these neurons also changes under persistent inflammatory pain conditions. Using patch-clamp recording combined with imaging cobalt staining, we found that, normal conditions, an pool AMPARs in rat substantia gelatinosa (SG) predominantly consists GluR2-containing Ca(2+)-impermeable...
Transient receptor potential ankyrin 1 (TRPA1) and melastatin 3 (TRPM3) are transduction channels of sensory neurons that play major roles in peripheral mechanisms somatosensation, including thermosensation, chemosensation nociception. Recent studies suggest both also contribute to central pain processing at the spinal cord level. TRPA1 TRPM3 highly permeable for Ca2+, suggesting they could regulate Ca2+ signalling synapses. However, information about TRPA1- TRPM3-induced dorsal horn (DH) is...
P2X3 purinoreceptors expressed in mammalian sensory neurons play a key role several processes, including pain perception. From the venom of Central Asian spider Geolycosa sp., we have isolated novel peptide, named purotoxin-1 (PT1), which is to our knowledge first natural molecule exerting powerful and selective inhibitory action on receptors. PT1 dramatically slows down removal desensitization these The peptide demonstrates potent antinociceptive properties animal models inflammatory pain.
Background Previous studies have shown that increased excitability of capsaicin-sensitive DRG neurons and thermal hyperalgesia in rats with short-term (2–4 weeks) streptozotocin-induced diabetes is mediated by upregulation T-type Ca 2+ current. In longer–term (after the 8th week) changed to hypoalgesia accompanied downregulation current small-sized nociceptors. At same time pain symptoms diabetic neuropathy other than persist STZ-diabetic animals patients during progression into later stages...
Persistent peripheral inflammation alters trafficking of AMPA receptors (AMPARs) at the synapses between primary afferents and dorsal horn (DH) neurons that contribute to maintenance inflammatory pain. However, whether changes synaptic activity within DH circuitry how it modulates AMPARs in different neuronal types still remain unknown. We find complete Freund adjuvant (CFA)-induced prominently augments excitatory neurotransmission rat lamina II characterized by intrinsic adapting firing...
Persistent peripheral inflammation changes AMPA receptor (AMPAR) trafficking in dorsal horn neurons by promoting internalization of GluR2-containing, Ca(2+)-impermeable AMPARs from the synapses and increasing insertion GluR1-containing, Ca(2+)-permeable extrasynaptic plasma membrane. These contribute to maintenance persistent inflammatory pain. However, much less is known about AMPAR during development pain direct studies functioning this period are still lacking. Using Complete Freund's...
The dorsal horn (DH) neurons of the spinal cord play a critical role in nociceptive input integration and processing central nervous system. Engaged neuronal classes cell-specific excitability shape computation within DH. DH hyperexcitability (central sensitisation) has been considered fundamental mechanism mediating hypersensitivity, with proven Ca2+-permeable AMPA receptors (AMPARs). However, whether how relates to changes action potential (AP) parameters if AMPARs contribute these remain...
Cannabinoid receptors (CBRs) belong to G protein-coupled receptor superfamily, which activation in salivary cells inhibits agonist-stimulated salivation and modifies saliva content. However, the role of different CBR subtypes acinar cell physiology intracellular signalling remains unclear. Here, we uncover functioning CB1Rs CB2Rs rat submandibular gland their essential secretion. Pharmacological suppressed outflow modified content produced by vivo. Using Na+-selective microelectrodes record...
Persistent pain remains a major health issue: common treatments relying on either repeated local injections or systemic drug administration are prone to concomitant side-effects. It is thought that an alternative could be multifunctional cargo system deliver medicine the target site and release it over prolonged time window. We nano-engineered microcapsules equipped with adjustable properties encapsulated sodium-channel blocker QX-314 using layer-by-layer (LbL) technology. First, we employed...
Upregulation of Ca(2+)-permeable AMPA receptors (CP-AMPARs) in the dorsal horn (DH) neurons spinal cord has been causally linked to maintenance persistent inflammatory pain. Therefore, inhibition CP-AMPARs could potentially alleviate an, otherwise, poorly treatable chronic However, a loss produce considerable side effects because crucial role synaptic plasticity. Here we have tested whether with dicationic compounds, open-channel antagonists acting an activity-dependent manner, can relieve...