A5009701990- CRISPR and Genetic Engineering
- Genetically Modified Organisms Research
- Antibiotic Resistance in Bacteria
- Identification and Quantification in Food
- Bacterial Identification and Susceptibility Testing
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Recycling and Waste Management Techniques
- Insect Resistance and Genetics
- Pharmaceutical and Antibiotic Environmental Impacts
- Neurogenesis and neuroplasticity mechanisms
- Protein Tyrosine Phosphatases
- Animal testing and alternatives
- Biosimilars and Bioanalytical Methods
- Microtubule and mitosis dynamics
- Mitochondrial Function and Pathology
- Pluripotent Stem Cells Research
- Contact Dermatitis and Allergies
- Neuroblastoma Research and Treatments
- Transgenic Plants and Applications
- Microplastics and Plastic Pollution
- Ichthyology and Marine Biology
- Molecular Biology Techniques and Applications
- Axon Guidance and Neuronal Signaling
- Animal Disease Management and Epidemiology
Eurostat
2020-2022
Publications Office of the European Union
2021-2022
European Commission
2018-2020
Directorate-General Joint Research Centre
2019
Joint Research Centre
2011-2019
University of Helsinki
2006-2012
Montreal Neurological Institute and Hospital
2002-2010
McGill University
1999-2006
Molecular Oncology (United States)
1999
Massachusetts Institute of Technology
1999
In this article, we show that, in transfected COS-1 cells, protein tyrosine phosphatase (PTP)-PEST translocates to the membrane periphery following stimulation by extracellular matrix fibronectin. When plated on fibronectin, PTP-PEST (−/−) fibroblasts display a strong defect motility. 3 h after plating number and size of vinculin containing focal adhesions were greatly increased homozygous mutant cells as compared with heterozygous cells. This phenomenon appears be due part constitutive...
Glial cell line–derived neurotrophic factor (GDNF) and hepatocyte growth (HGF) are multifunctional signaling molecules in embryogenesis. HGF binds to activates Met receptor tyrosine kinase. The complex for GDNF typically includes both family α1 (GFRα1) Ret can also signal independently of via GFRα1, although the mechanism has remained unclear. We now show that partially restores ureteric branching morphogenesis ret-deficient mice with severe renal hypodysplasia. Ret-independent effect was...
K252a is best known as a Trk inhibitor, but also neuroprotective compound. CEP1347, derivative, retains properties, does not inhibit TrkA. CEP1347 has recently been shown to directly MAPKKKs, including MLK3, the effect of on MAPKKKs remains unknown. and only prevent death, facilitate neurite outgrowth maintenance, somal hypertrophy, neurotransmitter synthesis. The biochemical basis for these trophic effects We have compared MLK JNK signaling neurotrophic pathways that support survival...
Malignant glioma is the major brain tumor in adults and has a poor prognosis. The failure to control invasive cell subpopulations may be key reason for local recurrence after radical resection contribute substantially of other treatment modalities such as radiation therapy chemotherapy. As model this invasion, we have implanted spheroids from human line (U251) three-dimensional collagen type I matrices, which these cells readily invade. We first observed that Src family kinase-specific...
The polymerase chain reaction (PCR) is the current state of art technique for DNA-based detection Genetically Modified Organisms (GMOs). A typical control strategy starts by analyzing a sample presence target sequences (GM-elements) known to be present in many GMOs. Positive findings from this "screening" are then confirmed with GM (event) specific test methods. reliable knowledge which GMOs detected combinations GM-detection methods thus crucial minimize verification efforts.In article, we...
With the growing number of GMOs introduced to market, testing laboratories have seen their workload increase significantly. Ready-to-use multi-target PCR-based detection systems, such as pre-spotted plates (PSP), reduce analysis time while increasing capacity. This paper describes development and applicability GMO a screening strategy involving PSP its associated web-based Decision Support System. The was developed detect all authorized in EU one single PCR experiment, through combination 16...
<ns4:p>Next-Generation Sequencing (NGS) technologies are expected to play a crucial role in the surveillance of infectious diseases, with their unprecedented capabilities for characterisation genetic information underlying virulence and antimicrobial resistance (AMR) properties microorganisms. In implementation any novel technology regulatory purposes, important considerations such as harmonisation, validation quality assurance need be addressed. NGS pose unique challenges these regards,...
Many food and feed additives result from fermentation of genetically modified (GM) microorganisms. For vitamin B2 (riboflavin), GM Bacillus subtilis production strains have been developed are often used. The presence neither the strain nor its recombinant DNA is allowed for products placed on EU market as or additive. A product (80% grade) imported China was analysed. Viable B. cells were identified DNAs two bacterial isolates (LHL LGL) subjected to three whole genome sequencing (WGS) runs...
Signal transduction pathways are often seen as cascades of kinases, whereas phosphatases relinquished to the housekeeping function resetting individual elements a resting state. However, critical biological processes such cellular migration require coordinated and constant remodeling actin cytoskeleton well rapid turnover cell-substratum linkages that necessitate concomitant action antagonistic enzymes. Tyrosine phosphorylation was long known be involved in adhesion de-adhesion mediated via...
The mechanisms underlying the decision of a stem or progenitor cell to either self-renew differentiate are incompletely understood. To address role Myc in this process, we expressed different forms proto-oncogene multipotent neural cells (NPCs) using retroviral transduction. Expression neurospheres increased proportion self-renewing fivefold, and 1% Myc-overexpressing cells, but none control retained self-renewal capacity even under differentiation-inducing conditions. A mutant (MycV394D)...
Abstract The application of new data streams generated from next-generation sequencing (NGS) has been demonstrated for food microbiology, pathogen identification, and illness outbreak detection. establishment best practices integrity,reproducibility, traceability will ensure reliable, auditable, transparent processes underlying microbiology risk management decisions. We outline general principles to guide the use NGS in support microbiological safety. Regulatory authorities across intra-...
The development of an efficient seafood traceability framework is crucial for the management sustainable fisheries and monitoring potential substitution fraud across food chain. Recent studies have shown DNA barcoding methods in this framework, with most efforts focusing on using mitochondrial targets such as cytochrome oxidase 1 b genes. In article, we show identification novel nuclear genome, their associated primers, to be used flatfishes Pleuronectidae family. addition, different silico...
Gadoids are a group of fish with historical importance in the fishing industry. The high demand for cod is one reasons why products often mislabelled, and numerous observations have been made on replacement Atlantic (Gadus morhua) by cheaper species or its illegal capture contravention quotas. Fish identification traditionally based morphological features, but this may be difficult case heat-treated processed products, where look similar, as Gadoid group. DNA-based approaches (using either...
<ns4:p>Next-Generation Sequencing (NGS) technologies are expected to play a crucial role in the surveillance of infectious diseases, with their unprecedented capabilities for characterisation genetic information underlying virulence and antimicrobial resistance (AMR) properties microorganisms. In implementation any novel technology regulatory purposes, important considerations such as harmonisation, validation quality assurance need be addressed. NGS pose unique challenges these regards,...
Signal transduction pathways are often seen as cascades of kinases, whereas phosphatases relinquished to the housekeeping function resetting individual elements a resting state. However, critical biological processes such cellular migration require coordinated and constant remodeling actin cytoskeleton well rapid turnover cell-substratum linkages that necessitate concomitant action antagonistic enzymes. Tyrosine phosphorylation was long known be involved in adhesion de-adhesion mediated via...
The DNA target sequence is the key element in designing detection methods for genetically modified organisms (GMOs). Unfortunately this information frequently lacking, especially unauthorized GMOs. In addition, patent sequences are generally poorly annotated, buried complex and extensive documentation hard to link corresponding GM event. Here, we present JRC GMO-Amplicons, a database of amplicons collected by screening public nucleotide databanks silico determination PCR amplification with...
Next Generation Sequencing technologies significantly impact the field of Antimicrobial Resistance (AMR) detection and monitoring, with immediate uses in diagnosis risk assessment. For this application general, considerable challenges remain demonstrating sufficient trust to act upon meaningful information produced from raw data, partly because reliance on bioinformatics pipelines, which can produce different results therefore lead interpretations. With constant evolution field, it is...
Knowledge of the number DNA sequences targeted by taxon-specific reference assays is essential for correct GM quantification and key to harmonisation measurement results. In present study droplet digital PCR (ddPCR) was used determine target copies validated real-time four main genetically modified (GM) crops. The transferability experimental conditions from ddPCR also explored, as well effect digestion. results this indicate that each crop at least one assay can be identified having a...