A5003466841- Diphtheria, Corynebacterium, and Tetanus
- RNA and protein synthesis mechanisms
- Antibiotic Resistance in Bacteria
- RNA Research and Splicing
- Clostridium difficile and Clostridium perfringens research
- RNA modifications and cancer
- Veterinary medicine and infectious diseases
- Toxin Mechanisms and Immunotoxins
- Infections and bacterial resistance
- Transgenic Plants and Applications
- Influenza Virus Research Studies
- Genomics and Chromatin Dynamics
- Burkholderia infections and melioidosis
- Invertebrate Taxonomy and Ecology
- Liver Disease Diagnosis and Treatment
- Bacterial Identification and Susceptibility Testing
- Plant Pathogenic Bacteria Studies
- Escherichia coli research studies
- Antimicrobial Resistance in Staphylococcus
- Hepatitis B Virus Studies
- Force Microscopy Techniques and Applications
- Tuberculosis Research and Epidemiology
- Genetically Modified Organisms Research
- Respiratory viral infections research
- Enterobacteriaceae and Cronobacter Research
FHNW University of Applied Sciences and Arts
2023-2025
Areté Associates (United States)
2023
University of Basel
2017-2019
University Hospital of Basel
2016-2019
Roche (Switzerland)
2019
Ludwig-Maximilians-Universität München
2012-2015
Gene Therapy Laboratory
2012
Center for Integrated Protein Science Munich
2012
University of Regensburg
2009
Toxigenic Corynebacterium diphtheriae is an important and potentially fatal threat to patients public health. During the current dramatic influx of refugees into Europe, our objective was use whole genome sequencing for characterization a suspected outbreak C. wound infections among refugees. After conventional culture, we identified using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) investigated toxigenicity by PCR. Whole performed on MiSeq Illumina with...
Messenger RNA (mRNA) synthesis and export are tightly linked, but the molecular mechanisms of this coupling largely unknown. In Saccharomyces cerevisiae, conserved TREX complex couples transcription to mRNA mediates mRNP formation. Here, we show that is recruited machinery by direct interaction its subcomplex THO with serine 2-serine 5 (S2/S5) diphosphorylated CTD polymerase II. S2 and/or tyrosine 1 (Y1) phosphorylation required for occupancy in vivo, establishing a second platform necessary...
Toxigenic Corynebacterium ulcerans can cause a diphtheria-like illness in humans and have been found domestic animals, which were suspected to serve as reservoirs for zoonotic transmission. Additionally, toxigenic C. reported take over the leading role causing diphtheria last years many industrialized countries.To gain deeper insights into tox gene locus understand transmission pathway detail, we analyzed nine isolates derived from human patients their animals applying next generation...
Efflux pumps mediate antimicrobial resistance in several WHO critical priority bacterial pathogens. However, most available data come from laboratory strains. The quantitative relevance of efflux more relevant clinical isolates remains largely unknown. We developed a versatile method for genetic engineering multi-drug resistant (MDR) bacteria, and used this to delete tolC specific antibiotic-resistance genes 18 representative MDR E. coli isolates. determined activity minimal inhibitory...
ABSTRACT The spread of antibiotic resistance is driving interest in new approaches to control bacterial pathogens. This includes applying multiple antibiotics strategically, using bacteriophages against antibiotic-resistant bacteria, and combining both types antibacterial agents. All these rely on or are impacted by associations among phenotypes (where bacteria resistant one agent also relatively susceptible others). Experiments with laboratory strains have shown strong between some...
Many food and feed additives result from fermentation of genetically modified (GM) microorganisms. For vitamin B2 (riboflavin), GM Bacillus subtilis production strains have been developed are often used. The presence neither the strain nor its recombinant DNA is allowed for products placed on EU market as or additive. A product (80% grade) imported China was analysed. Viable B. cells were identified DNAs two bacterial isolates (LHL LGL) subjected to three whole genome sequencing (WGS) runs...
Introduction Extended-spectrum beta-lactamases (ESBL)-producing Enterobacteriaceae were first described in relation with hospital-acquired infections. In the 2000s, epidemiology of ESBL-producing organisms changed as especially Escherichia coli was increasingly an important cause community-acquired infections, supporting hypothesis that more recent years have probably been imported into hospitals rather than vice versa. Transmission is complicated by ESBL genes being encoded on...
Characterizing biomolecular receptor–ligand interactions is critical for research and development. However, performing analyses in complex, biologically relevant matrices, such as serum, remains challenging due to non-specific binding that often impairs measurements. Here, we evaluated Focal Molography (FM) determining KD kinetic constants comparison gold-standard methods using single-domain heavy-chain antibodies various systems. FM provided highly comparable SPR BLI standard buffers...
Mutations in WD repeat domain 36 gene (WDR36) play a causative role some forms of primary open-angle glaucoma, leading cause blindness worldwide. WDR36 is characterized by the presence multiple WD40 repeats and shows homology to Utp21, an essential protein component yeast small subunit (SSU) processome required for maturation 18S rRNA. To clarify functional mammalian organism, we generated investigated mutant mice with targeted deletion Wdr36. In parallel experiments, used RNA interference...
Human-to-human-transmitted Corynebacterium diphtheriae was historically the main pathogen causing diphtheria and has therefore been studied extensively in past. More recently, caused by toxigenic ulcerans is an emerging disease several industrial countries, including United Kingdom, States, France, Germany. However, C. so far almost neglected development of epidemiologic tools. One most important tools modern epidemiology to understand transmission pathways sequence typing pathogens. Here,...
Background The recent crisis of refugees seeking asylum in European countries challenges public health on many levels. Most currently arrive from Syria, Afghanistan, or Eritrea. Data about multidrug resistant bacteria (MDR) prevalence are not present for these countries. However, when entering the heath care systems, data colonisation rates regarding highly bacterial pathogens important. Methods We performed a cross-sectional screening four Swiss refugee centres to determine colonization...
Severe necrotizing fasciitis was diagnosed in a 53-year-old man Germany 2012. Toxigenic Corynebacterium ulcerans grown from wound swab sample. One of the patient's 2 dogs found to harbor toxigenic C. strain. Results next generation sequencing both isolates supported recent zoonotic transmission this bacterial pathogen.
Abstract The origin and population structure of Borrelia burgdorferi sensu stricto (s.s.), the agent Lyme disease, remain obscure. This tick-transmitted bacterial species occurs in both North America Europe. We sequenced 17 European isolates (representing most frequently found sequence types Europe) compared these with American strains. show that trans-Atlantic exchanges have occurred evolutionary history this a B. s.s. is marginally more likely than USA origin. data further suggest some...
The La-motif (LAM) is an ancient and ubiquitous RNA-binding domain defining a superfamily of proteins, which comprises the genuine La proteins La-related (LARPs). In contrast to La, binds stabilizes pre-tRNAs other RNA polymerase III transcripts, data on function targets LARPs have remained scarce. We undertaken global approach elucidate previously suggested role yeast LARP Slf1p in copper homeostasis. By applying protein immunopurification-microarray (RIP-Chip) analysis, we show that its...
RNA polymerase III (RNAPIII) synthesizes most small RNAs, the prominent being tRNAs. Although basic mechanism of RNAPIII transcription is well understood, recent evidence suggests that additional proteins play a role in transcription. Here, we discovered by genome-wide approach Nab2, poly(A)-binding protein important for correct poly(A) tail length and nuclear mRNA export, present at all transcribed genes. The occupancy Nab2 genes dependent on Using novel temperature-sensitive allele NAB2 ,...
We describe an outbreak of Burkholderia stabilis associated with contaminated washing gloves, a commercially available Class I medical device. Triggered by increase in cepacia complex (BCC) bacteremias and the detection BCC unopened packages ad hoc national committee comprising representatives public health organisation, regulatory agency, expert association convened commissioned investigation. The investigation included retrospective case finding across Switzerland whole genome sequencing...
Organs transplanted across donor‐specific HLA antibodies (DSA) are associated with a variety of clinical outcomes, including high risk acute kidney graft rejection. Unfortunately, the currently available assays to determine DSA characteristics insufficient clearly discriminate between potentially harmless and harmful DSA. To further explore hazard potential DSA, their concentration binding strength natural target, using soluble HLA, may be informative. There number biophysical technologies...
During transcription of protein coding genes by RNA Polymerase II the mRNA is processed and packaged into an mRNP. Among proteins binding cotranscriptionally to mRNP are export factors. One complexes thus coupling TREX complex. However, despite fact that was identified characterized about a decade ago, it had remained enigmatic how recruited genes. The conserved Prp19 complex (Prp19C) has long been known for its function in splicing. We recently Prp19C be essential second step gene...