A5021621479- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Genetically Modified Organisms Research
- COVID-19 diagnosis using AI
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Insect Resistance and Genetics
- Plant Disease Resistance and Genetics
- Identification and Quantification in Food
- Biosensors and Analytical Detection
- Molecular Biology Techniques and Applications
- COVID-19 Clinical Research Studies
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Plant Pathogenic Bacteria Studies
- Plant and Fungal Interactions Research
- Plant tissue culture and regeneration
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Transgenic Plants and Applications
- Biosimilars and Bioanalytical Methods
- Insect-Plant Interactions and Control
- Bacterial Identification and Susceptibility Testing
- Nicotinic Acetylcholine Receptors Study
- Dermatological and COVID-19 studies
Joint Research Centre
2011-2025
European Commission
2007-2022
International Potato Center
1993-2000
Istituto Sperimentale per la Zootecnia
1988
Rx2 confers resistance against potato virus X (PVX). To clone Rx2, we developed a system based on Agrobacterium-mediated transient expression of candidate R genes in transgenic tobacco leaves expressing the PVX coat protein elicitor Rx2-mediated resistance. Using this system, gene eliciting HR specifically presence was identified. Based genetical and functional analysis, it is concluded that cloned Rx2. The potentially adaptable to cloning any other gene. locus chromosome V encoded highly...
Digital PCR is rapidly being adopted in the field of DNA-based food analysis. The direct, absolute quantification it offers makes an attractive technology for routine analysis and feed samples their composition, possible GMO content, compliance with labelling requirements. However, assessing performance dPCR assays not yet well established. This article introduces three straightforward parameters based on statistical principles that allow users to evaluate if are robust. In addition, we...
Multiple lineages of SARS-CoV-2 have been identified featuring distinct sets genetic changes that confer to the virus higher transmissibility and ability evade existing immunity. The continuous evolution may pose challenges for current treatment options diagnostic tools. In this study, we first evaluated performance 14 WHO-recommended real-time reverse transcription (RT)-PCR assays currently in use detection found only one assay has reduced against Omicron. We then developed a new duplex...
<ns4:p><ns4:bold>Background</ns4:bold></ns4:p><ns4:p> Scientific evidence for the involvement of human microbiota in development COVID-19 disease has been reported recently. SARS-CoV-2 RNA presence faecal samples and activity faeces from patients have observed.</ns4:p><ns4:p> <ns4:bold>Methods</ns4:bold></ns4:p><ns4:p> Starting these observations, an experimental design was developed to cultivate <ns4:italic>in vitro</ns4:italic> infected individuals, monitor SARS-CoV-2, collect data on...
Abstract Motivation Recommendations on the use of genomics for pathogens surveillance are evidence that high-throughput genomic sequencing plays a key role to fight global health threats. Coupled with bioinformatics and other data types (e.g., epidemiological information), is used obtain knowledge pathogenic threats insights their evolution, monitor spread, evaluate effectiveness countermeasures. From decision-making policy perspective, it essential ensure entire process’s quality before...
Summary Sporadic outbreaks of potato yellow vein disease (PYVD) were first observed in the early 1940's by growers Antioquia, Colombia. Long known to be transmitted greenhouse whitefly (Trialeurodes vaporariorum) , precise identity its causal agent (presumably viral nature) has remained obscure. Here, we present evidence that a closterovirus with bipartite genome, virus (PYVV), is associated PYVD. Electrophoretic analysis revealed diseased tissue contains 4–5 disease‐specific dsRNAs ranging...
With the growing number of GMOs introduced to market, testing laboratories have seen their workload increase significantly. Ready-to-use multi-target PCR-based detection systems, such as pre-spotted plates (PSP), reduce analysis time while increasing capacity. This paper describes development and applicability GMO a screening strategy involving PSP its associated web-based Decision Support System. The was developed detect all authorized in EU one single PCR experiment, through combination 16...
<ns4:p><ns4:bold>Background</ns4:bold></ns4:p><ns4:p> Scientific evidence for the involvement of human microbiota in development COVID-19 disease has been reported recently. SARS-CoV-2 RNA presence faecal samples and activity faeces from patients have observed.</ns4:p><ns4:p> <ns4:bold>Methods</ns4:bold></ns4:p><ns4:p> Starting these observations, an experimental design was developed to cultivate <ns4:italic>in vitro</ns4:italic> infected individuals, monitor SARS-CoV-2, collect data on...
The aphid Myzus persicae (Sulz.) was shown to transmit potato spindle tuber viroid (PSTVd) clone DTO-33 from source plants doubly infected with leafroll virus (PLRV) and PSTVd. Transmission of the persistent type did not occur when insects were allowed feed on singly plants. Only low levels PSTVd associated purified PLRV virions, but its resistance digestion micrococcal nuclease indicates that RNA is encapsidated within particles. Epidemiological surveys carried out at three locations in...
The development of an efficient seafood traceability framework is crucial for the management sustainable fisheries and monitoring potential substitution fraud across food chain. Recent studies have shown DNA barcoding methods in this framework, with most efforts focusing on using mitochondrial targets such as cytochrome oxidase 1 b genes. In article, we show identification novel nuclear genome, their associated primers, to be used flatfishes Pleuronectidae family. addition, different silico...
Background: SARS-CoV-2 that causes COVID-19 disease and led to the pandemic currently affecting world has been broadly investigated. Different studies have performed understand infection mechanism, involved human genes, transcripts proteins. In parallel, numerous clinical extra-pulmonary manifestations co-occurring with reported evidence of their severity persistence is increasing. Whether these are linked other disorders infection, under discussion. this work, we report identification...
The aim of this study was to develop a RT-PCR assay for the specific detection SARS-CoV-2 Omicron Variant Concern (VOC) as rapid alternative sequencing.
Extracts of Mirabilis jalapa (Nyctaginaceae), containing a ribosome inactivating protein (RIP) called antiviral (MAP), were tested against infection by potato virus X, Y, leaf roll virus, and spindle tuber viroid. Root extracts M. sprayed on test plants 24 h before or viroid inoculation inhibited almost 100%, as corroborated infectivity assays the nucleic acid spot hybridization test. Antiviral activity MAP was observed mechanically transmitted viruses but not aphid-transmitted viruses....
Different potato genotypes expressing extreme resistance to virus X were inoculated with strains cp, CP4, and HB three PVX mutants constructed in vitro. Using these viral mutants, the involvement of coat protein, specifically single amino acid residue at position 121 within this resistance-breaking capacity strain was confirmed for all genes tested. We concluded that expressed Solanum x chaucha, S. curtilobum, juzepczukii, vernei is conferred by Rx (RX cha , RX cur juz vrn respectively)...
The past two decades have witnessed the rise of commercial crops that been genetically modified for an increased suitability in extensive cultivation. Currently, a substantial body research is being carried out order to produce Genetically Modified (GM) animals may similarly yield improvements animal breeding, genetics and reproduction. Here, we attempt comprehensive review existing trails at modification with applications aimed deliberate release onto market. In addition, investigate...
<ns4:p><ns4:bold>Background:</ns4:bold> In previous studies we have shown that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replicates <ns4:italic>in vitro</ns4:italic> in bacterial growth medium, the viral replication follows growth, and it is influenced by administration of specific antibiotics. These observations are compatible with a ‘bacteriophage-like’ behaviour SARS-CoV-2. </ns4:p><ns4:p> <ns4:bold>Methods:</ns4:bold> We further elaborated on these unusual findings...
An innovative covalent microsphere immunoassay, based on the usage of fluorescent beads coupled to a specific antibody, was developed for quantification endotoxin Cry1Ab present in MON810 and Bt11 genetically modified (GM) maize lines. In particular, protocol assess presence very broad range GM concentrations, from 0.1 100% [weight organism (GMO)/weight[. Test linearity achieved values 3%, whereas fluorescence signal increased following nonlinear model, reaching plateau at 25%. The limits...
Potato spindle tuber viroid (PSTVd) was detected in avocado trees growing at several locations Peru by using nucleic acid spot hybridization assays. A viroidlike with a mobility similar to that of PSTVd also found return polyacrylamide gel electrophoresis analysis RNA extracted from trees, and mild strain recovered Nicandra physalodes inoculated leaf sap affected avocados. Sequence cDNAs obtained the polymerase chain reaction primers indicated presence three different sequence variants...
Abstract In Europe, a growing interest for reliable techniques the quantification of genetically modified component(s) food matrixes is arising from need to comply with European legislative framework on novel products. Real-time polymerase chain reaction (PCR) currently most powerful technique specific nucleic acid sequences. Several real-time PCR methodologies based different molecular principles have been developed this purpose. The frequently used approach in field organism (GMO) or feed...