A5076571419- Renal and related cancers
- Cell Adhesion Molecules Research
- Urological Disorders and Treatments
- Nerve injury and regeneration
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Renal cell carcinoma treatment
- Wnt/β-catenin signaling in development and cancer
- Neurogenesis and neuroplasticity mechanisms
- Genetic and Kidney Cyst Diseases
- Reproductive Biology and Fertility
- Neuroblastoma Research and Treatments
- Organ Donation and Transplantation
- Congenital gastrointestinal and neural anomalies
- Sperm and Testicular Function
- Congenital heart defects research
- Birth, Development, and Health
- Cardiac and Coronary Surgery Techniques
- Renal Diseases and Glomerulopathies
- Axon Guidance and Neuronal Signaling
- Signaling Pathways in Disease
- Animal Genetics and Reproduction
- Tracheal and airway disorders
- RNA Research and Splicing
- Reproductive System and Pregnancy
University of Helsinki
2011-2024
Helsinki University Hospital
2004-2021
Czech Academy of Sciences, Institute of Biotechnology
1997-2015
Children's Hospital
2013
Institute for Biomedicine
2003-2008
Biocenter Finland
1994-2002
Foundation for Growth Science
2002
Tampere University
1999
Helsinki Children's Hospital
1991-1998
Helsinki Institute of Physics
1995-1998
The growth and differentiation factor transforming factor-beta2 (TGFbeta2) is thought to play important roles in multiple developmental processes. Targeted disruption of the TGFbeta2 gene was undertaken determine its essential role vivo. TGFbeta2-null mice exhibit perinatal mortality a wide range defects for single disruption. These include cardiac, lung, craniofacial, limb, spinal column, eye, inner ear urogenital defects. processes most commonly involved affected tissues...
The molecular control of self-renewal and differentiation stem cells has remained enigmatic. Transgenic loss-of-function overexpression models now show that the dosage glial cell line–derived neurotrophic factor (GDNF), produced by Sertoli cells, regulates fate decisions undifferentiated spermatogonial include for spermatogenesis. Gene-targeted mice with one GDNF -null allele depletion reserves, whereas overexpressing accumulation spermatogonia. They are unable to respond properly signals...
Embryonic stem cells (ESC) have been established previously from the inner cell mass of mouse blastocysts. In suspension culture, they spontaneously differentiate to blood-island-containing cystic embryoid bodies (CEB). The development blood vessels in situ differentiating endothelial islands, a process which we call vasculogenesis, was induced by injecting ESC into peritoneal cavity syngeneic mice. peritoneum, fusion islands and formation an vivo-like primary capillary plexus occurred....
ABSTRACT The shapes of different organs can be explained largely by two fundamental characteristics their epithelial rudiments – the pattern branching and rate pro-liferation. Glial-cell-line-derived neurotrophic factor (GDNF) has recently been implicated in development metanephric ureteric epithelium (Pichel, J. G., Shen, L., Sheng, H. Z., Granholm, A.-C., Drago, J., Grinberg, A., Lee, E. Huang, S. P., Saarma, M., Hoffer, B.J., Sariola, Westphal, (1996). Nature 382, 73-76; Sánchez, M.P.,...
The primary structure of the human laminin M chain was determined from cDNA clones isolated placental libraries. covered a total 6,942 bp, with 49-bp encoding 5' end untranslated region and 6,893-bp coding for translated sequence. complete contains 22-residue signal peptide 3,088 residues mature chain. has domain similar to that mouse A chains. homology between two heavy chains is highest in short arm lowest long helical I + II. Northern blot analysis fetal tissues showed expressed most such...
We describe the identification of a novel laminin chain. Overlapping clones were isolated from human fibrosarcoma HT1080 cell cDNA library spanning total 5,200 bp. A second set contained an alternative 3' end sequence giving 4,316 The longer open reading frame for 1,193-residue-long polypeptide. was shortened at carboxyl-terminal coding 1,111-residue-long amino acid 21 acids putative signal peptide and 1,172 residues or alternatively 1,090 with five distinct domains homologous to I-V in...
Nerve growth factor receptor (NGFR) serves as the binding site for neurotrophic factors. Although NGFR has been found in several embryonic tissues outside nervous system, function of embryogenesis non-neuronal organs remains unknown. is transiently synthesized by rat kidney and disappears from nephrons upon their terminal differentiation. Anti-sense oligonucleotide inhibition expression inhibits morphogenesis. Therefore, required not only development but also differentiation tubules.
We have identified a synthetic peptide derived from the B2‐chain of mouse laminin, Arg‐Asn‐Ile‐Ala‐Glu‐Ile‐Ile‐Lys‐Asp‐Ile (p20), which simulates neurite outgrowth‐promoting activity native molecule. In organotypic cultures, neurons newborn brain or embryonic peripheral nervous system responded by extensive outgrowth for laminin p20 in culture medium. If rat cerebellar were grown on 1–5 μM (1–5 μg/ml) medium competed with and inhibited neuronal attachment outgrowth, whereas higher...
The primary structure of human macrophage receptor with collagenous (MARCO) was determined from cDNA clones and shown to be highly similar that mouse (Elomaa, O., Kangas, M., Sahlberg, C., Tuukkanen, J., Sormunen, R., Liakka, A., Thesleff, I., Kraal, G., Tryggvason, K. (1995)<i>Cell</i> 80, 603–609). Features such as potential carbohydrate attachment sites in the extracellular spacer domain III interruption Gly-Xaa-Yaa repeats IV were conserved between two species. However, MARCO polypeptide...
Wnt proteins are required for induction of nephrons in mouse metanephric kidneys, but the downstream pathways that mediate tubule and epithelial differentiation have remained obscure. The intracellular mechanisms by which signaling mediates nephron embryonic kidney mesenchymes were studied. First is shown transient exposure isolated to structurally different glycogen synthase kinase-3 (GSK3) inhibitors lithium or 6-bromoindirubin-3′-oxime results abundant full segregation nephrons. Shown...
Abstract The localization of glial cell line‐derived neurotrophic factor (GDNF) mRNA was studied by in situ hybridization rat from embryonic (E) day E10 to E15. At E10, GDNF is found the urogenital field and cranial part gut. E11, most abundant expression seen epithelial cells second, third fourth pharyngeal pouches, arches pharynx. Also mesenchymal gut mesonephric tubules contain mRNA. E13, observed layers oesophagus, intestine stomach, around condensing cartilages metanephric kidney...
The complete primary structure of a novel human laminin α4 chain was derived from cDNA clones. translation product contains 24‐residue signal peptide preceding the mature 1792 residues. domain is similar to that recently described α3 [Ryan, Tizard, Van Devanter and Carter (1994) J. Biol. Chem. 269, 22779–22787]. Northern analysis RNA fetal adult tissues revealed developmental regulation expression. In adult, strong expression observed in heart as well lung, ovary, small large intestines,...