A5021855910- Cell Adhesion Molecules Research
- Cardiac electrophysiology and arrhythmias
- Immune Cell Function and Interaction
- Ion channel regulation and function
- Antimicrobial Peptides and Activities
- Proteoglycans and glycosaminoglycans research
- Protease and Inhibitor Mechanisms
- Glycosylation and Glycoproteins Research
- Lipid Membrane Structure and Behavior
- Cellular Mechanics and Interactions
- Skin and Cellular Biology Research
- Monoclonal and Polyclonal Antibodies Research
- Collagen: Extraction and Characterization
- Invertebrate Immune Response Mechanisms
- Viral-associated cancers and disorders
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- Cardiovascular Effects of Exercise
- Cardiomyopathy and Myosin Studies
- Neuropeptides and Animal Physiology
- RNA and protein synthesis mechanisms
- Protein Hydrolysis and Bioactive Peptides
- Caveolin-1 and cellular processes
- vaccines and immunoinformatics approaches
- Chemical Synthesis and Analysis
Lawrence Livermore National Laboratory
2024
Cardiff University
2012-2022
Hudson Institute
2019
New York Academy of Sciences
2019
Icahn School of Medicine at Mount Sinai
2019
John Wiley & Sons (United States)
2019
University Hospital of Wales
2017
Slovak Academy of Sciences
2011
Institute of Molecular Biology of the Slovak Academy of Sciences
2011
École Polytechnique Fédérale de Lausanne
2010
Large quantities of intact laminin-nidogen complex could be extracted from a mouse tumor basement membrane with physiological buffer containing EDTA. Analysis the purified demonstrated that two proteins occur in an equimolar ratio and anchoring these complexes to extracellular matrix requires divalent cations. Reversible dissociation was achieved 2 M guanidine X HCl has been used for purification individual components. Electron microscopy binding studies using laminin fragments nidogen...
A method is described that eliminates surface flow in monolayers at the air-water interface and makes possible diffusion measurements by fluorescence microphotolysis ("photobleaching"). In contrast to previous studies did not account for flow, lipid probe has been found be similar densely packed related bilayers. Furthermore, it seems based on same molecular mechanism monolayers, bilayers, potentially also cell membranes. of L-alpha-dilauroylphosphatidylcholine (Lau2-PtdCho) translational...
We describe the identification of a novel laminin chain. Overlapping clones were isolated from human fibrosarcoma HT1080 cell cDNA library spanning total 5,200 bp. A second set contained an alternative 3' end sequence giving 4,316 The longer open reading frame for 1,193-residue-long polypeptide. was shortened at carboxyl-terminal coding 1,111-residue-long amino acid 21 acids putative signal peptide and 1,172 residues or alternatively 1,090 with five distinct domains homologous to I-V in...
Mutations resulting in replacement of one obligate Gly residue within the repeating (Gly-Xaa-Yaa) n triplet pattern collagen type I triple helix are major cause osteogenesis imperfecta (OI). Phenotypes OI involve fragile bones and range from mild to perinatal lethal. In this study, host–guest triple-helical peptides form acetyl-(Gly-Pro-Hyp) 3 - Zaa -Pro-Hyp-(Gly-Pro-Hyp) 4 -Gly-Gly-amide used isolate influence replacing on triple-helix stability, with = Gly, Ala, Arg, Asp, Glu, Cys, Ser, or...
Transglutaminases (TG) form a family of enzymes that catalyse various post-translational modifications glutamine residues in proteins and peptides including intra- intermolecular isopeptide bond formation, esterification deamidation. We have characterized novel member the mammalian TG family, TG6, which is expressed human carcinoma cell line with neuronal characteristics mouse brain. Besides full-length protein, alternative splicing results short variant lacking second β-barrel domain man...
In chronic respiratory disease, the formation of dense, 3-dimensional "microcolonies" by Pseudomonas aeruginosa within airway plays an important role in contributing to resistance treatment. An vitro biofilm model pseudomonal microcolony using artificial-sputum (AS) medium was established study effects low-molecular-weight alginate oligomers (OligoG CF-5/20) on growth, formation, and efficacy colistin. The studies employed clinical cystic fibrosis (CF) isolates (n = 3) reference nonmucoid...
We identified a novel Drosophila protein of ≈ 400kDa, hemolectin (d-Hml), secreted from haemocyte-derived Kc167 cells. Its 11.7kbp cDNA contains an open reading frame 3843 amino acid residues, with conserved domains in von Willebrand factor (VWF), coagulation V/VIII and complement factors. The d-hml gene is located on the third chromosome (position 70C1-5) consists 26 exons. major part d-Hml well-known motifs organization:...
ABSTRACT The locomotion of single epidermis cells, grown out from Xenopus laevis tadpole tails has been investigated by time-lapse cinemicrography using phase-contrast and reflection-contrast optics. cells develop a large, mostly 200–250 nm thick, lamella, which adheres homogeneously to the supporting coverglass exceeds projection area cell body. From comparison RIC-pictures taken at high (1.06) low (0.62) numerical aperture illumination (I.N.A.) we deduce that I.N.A. embossment...
Antimicrobial peptides (AMPs) have therapeutic potential, particularly for localized infections such as those of the lung. Here we show that airway administration a pegylated AMP minimizes lung tissue toxicity while nevertheless maintaining antimicrobial activity. CaLL, potent synthetic (KWKLFKKIFKRIVQRIKDFLR) comprising fragments LL-37 and cecropin A peptides, was N-terminally (PEG-CaLL). PEG-CaLL derivatives retained significant activity (50% inhibitory concentrations [IC(50)s] 2- to...
ABSTRACT Pseudomonas aeruginosa plays a major role in many chronic infections. Its ability to readily form biofilms contributes its success as an opportunistic pathogen and resistance/tolerance antimicrobial/antibiotic therapy. A low-molecular-weight alginate oligomer (OligoG CF-5/20) derived from marine algae has previously been shown impair motility P. disrupt pseudomonal biofilm assembly. As these bacterial phenotypes are regulated by quorum sensing (QS), we hypothesized that OligoG...
Collagens contain a high amount of charged residues involved in triple-helix stability, fibril formation, and ligand binding. The contribution to stability was analyzed utilizing host-guest peptide system with single Gly-X-Y triplet embedded within Ac(Gly-Pro-Hyp)3-Gly-X-Y-(Gly-Pro-Hyp)4-Gly-Gly-NH2. ionizable Arg, Lys, Glu, Asp were incorporated into the X position Gly-X-Hyp; Y Gly-Pro-Y; or as pairs oppositely occupying positions. Gly-X-Hyp peptides had similar thermal stabilities, only...
Leech neurons in culture sprout rapidly when attached to extracts from connective tissue surrounding the nervous system. Laminin-like molecules that promote sprouting have now been isolated this extracellular matrix. Two mAbs prepared react on immunoblots with a approximately equal 220- and 340-kD polypeptide, respectively. These antibodies used purify cross-shaped structures electron microscope. The molecules, of 10(3) kD nonreducing SDS gels, subunits 340, 220, 160-180 kD. Attachment...
Keratinocyte migration during epidermal repair depends on interactions between cellular heparan sulfate proteoglycan receptors, syndecan-1 and -4, the C-terminal globular domains (LG45) of extracellular matrix protein laminin 332. This study investigates molecular basis binding specificity -4 receptors expressed by human keratinocytes. We used site-directed mutagenesis to alter a recombinant LG45 substituting most critical basic residues with glutamine. All proteins were in mammalian cells,...
The refolding of thermally denatured model collagen-like peptides was studied for a set 21 guest triplets embedded in common host framework: acetyl-(Gly-Pro-Hyp)3-Gly-Xaa-Yaa-(Gly-Pro-Hyp)4-Gly-Gly-amide. results show strong dependence the folding rate on identity Gly-Xaa-Yaa triplet, with half-times varying from 6 to 110 min (concentration = 1 mg/ml). All form Gly-Xaa-Hyp promoted rapid folding, only marginally dependent residue Xaa position. In contrast, Gly-Pro-Yaa and were slower showed...
We identified a novel Drosophila protein of ≈ 400kDa, hemolectin (d-Hml), secreted from haemocyte-derived Kc167 cells. Its 11.7kbp cDNA contains an open reading frame 3843 amino acid residues, with conserved domains in von Willebrand factor (VWF), coagulation V/VIII and complement factors. The d-hml gene is located on the third chromosome (position 70C1-5) consists 26 exons. major part d-Hml well-known motifs organization:...
The extracellular‐matrix glycoprotein, tenascin, consists of disulfide‐linked subunits 190, 200 and 230 kDa (the three splicing variants reported in chicken) usually exists as a six‐armed structure under the electron microscope. We used monoclonal antibodies to isolate characterize different proteolytic fragments obtained from native protein. Purified monomeric tenascin has molecular mass 216 is structured single arms. Tenascin by pepsin digestion bind antibody (mAb) TnM1 which directed...
In 40% of cases classical Hodgkin lymphoma (cHL), Epstein-Barr virus (EBV) latency-II antigens [EBV nuclear antigen 1 (EBNA1)/latent membrane protein (LMP)1/LMP2A] are present (EBV(+) cHL) in the malignant cells and presentation is intact. Previous studies have shown consistently that HLA-A*02 protective EBV(+) cHL, yet its role disease pathogenesis unknown. To explore basis for this observation, gene expression was assessed 33 cHL nodes. Interestingly, CD8 LMP2A were correlated strongly...
Presentation of identical HIV-1 peptides by closely related Human Leukocyte Antigen class I (HLAI) molecules can select distinct patterns escape mutation that have a significant impact on viral fitness and disease progression. The molecular mechanisms which HLAI micropolymorphisms induce differential within peptide epitopes remain unknown.