A5028599195- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Virus-based gene therapy research
- DNA and Nucleic Acid Chemistry
- Neurobiology and Insect Physiology Research
- RNA Interference and Gene Delivery
- Animal Genetics and Reproduction
- Acute Myeloid Leukemia Research
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Nanoparticle-Based Drug Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- MicroRNA in disease regulation
- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Developmental Biology and Gene Regulation
- Molecular Biology Techniques and Applications
- Physiological and biochemical adaptations
- Characterization and Applications of Magnetic Nanoparticles
- Pluripotent Stem Cells Research
- Laser Applications in Dentistry and Medicine
- Neuroendocrine regulation and behavior
- Neuropeptides and Animal Physiology
Engelhardt Institute of Molecular Biology
2015-2024
Institute of Gene Biology
2024
Institute of Higher Nervous Activity and Neurophysiology
2023
Yahoo (United Kingdom)
2018-2020
Russian Cancer Research Center NN Blokhin
2016
Russian Academy of Sciences
1980-2011
New York Blood Center
1995-2007
Axcella Health (United States)
2007
University of Cologne
1989
Institute of Molecular Genetics
1979-1988
A procedure for the construction of general cDNA libraries is described which based on amplification total in vitro. The first strand synthesized from RNA using an oligo(dT)-containing primer. After oligo(dG) tailing amplified by PCR two primers complementary to oligo(dA) and ends cDNA. For insertion into a vector controlled trimming 3' Klenow enzyme was used. Starting 10 J558L micron3 myeloma cells, approximately 10(5) fold. library containing 10(6) clones established 1/6 Screening with...
A high-resolution map for the arrangement of histones along DNA in nucleosome core particle has been determined by a sequencing procedure based on crosslinking to 5'-terminal fragments produced scission one strand at point crosslinking. The position identified measuring length crosslinked fragments. results demonstrate that each is arranged within several adjacent or dispersed segments little less than 10 nucleotides length. Histone-free intervals are located between these regular distances...
Abstract The SHOM method (Sequencing by Hybridization with Oligonucleotide Matrix) developed in 1988 is a new approach to nucleic acid sequencing hybridization an oligonucleotide matrix composed of array immobilized oligonucleotides. original proposed for had contain at least 65,536 octanucleotides. present work describes family matrices, which allows one reduce the number synthesized oligonucleotides 5–15 times without essentially decreasing resolving power method.
Journal Article Distribution of high mobility group proteins 1/2,E and 14/17 linker histones H1 H5 on transcribed non-transcribed regions chicken erythrocyte chromatin Get access Yuri V. Postnikov, Postnikov Search for other works by this author on: Oxford Academic PubMed Google Scholar Valentin Shick, Shick Alexander Belyavsky, Belyavsky Konstantin R. Khrapko, Khrapko L. Brodolin, Brodolin Tatjana A. Nikolskaya, Nikolskaya 1Institute Chemical Physics, USSR Academy SciencesMoscow 117977,...
A key component of Cdc2/Cdk2-activating kinase (CAK) is p40MO15, a protein subunit that phosphorylates the T161/T160 residues p34cdc2/p33cdk2. The level and activity p40MO15 were essentially constant during cleavage fertilised Xenopus eggs in growing mouse 3T3 cells, but serum starvation these cells reduced both p40MO15. Although endogenous did not vary cell cycle, we found bacterially expressed was activated more rapidly by M-phase extracts than interphase extracts. Bacterially...
A series of mono- and dinucleosomal DNAs characterized by an about ten-base periodicity in the size were revealed micrococcal nuclease digests Drosophila chromatin which have 180 +/- 5 base pair (bp) nucleosomal repeat. 20, 30, 40 bp spacers found to be predominant trimming DNA dinucleosomes core position. Among several identified mononucleosomes (MN), MN170, MN180 MN190 isolated from different sources (the figures indicate length bp). The presence 10, 30 long was shown these crosslinking...
A novel method for identification of differentially expressed genes has been developed. It is based on the consecutive restriction digestions 3′ terminal cDNA fragments to produce a fingerprint gene expression. molecules are synthesized using biotinylated oligo(dT) primer, digested with frequently cutting endonuclease and 3′-terminal isolated streptavidin microbeads. After amplification by PCR, Immobilized again beads, radiolabeled treated sequentially set endonucleases. The products...
A refined map for the linear arrangement of histones along DNA in nucleosomal core particles has been determined by DNA-protein crosslinking. On one strand 145-bp DNA, are aligned following order: (5') H2B25,35-H455,65-H375,85,95/H488-H2B105,11 5-H2A118-H3135,145/H2A145 (3') (the subscripts give approximate distance nucleotides main histone contacts from 5'-end). Hence, tetramer (H3,H4)2 and two dimers (H2A-H2B) arranged on double-stranded a symmetrical rather autonomous way:...
Heat shock proteins including the major stress protein HSP70 support intracellular homeostasis and prevent damage after a temperature increase other stressful environmental stimuli, as well during aging. We have shown earlier that prolonged administration of recombinant human to mice exhibiting Alzheimer's-like neurodegeneration sepsis reduces clinical manifestations these pathologies. Herein, we studied action on young aged mouse mesenchymal stem cells (MSCs) in culture. The results...
The increasing incidence of melanoma makes this cancer an important public health problem. Therapeutic resistance is still a major obstacle to the therapy patients with metastatic melanomas. aim study was develop cell line resistant DNA-alkylating agents and elucidate mechanisms involved in acquired drug resistance. We established unique subline Mel MeR aranoza by continuous stepwise selection Me/WT concentrations drug. cells were also cross-resistant streptozotocin or cisplatin. Here, we...
Accumulating evidence suggests that mesenchymal stromal cells (MSCs) are recruited to the tumor, and promote tumor development growth. The present study was performed investigate communication between aggressive melanoma MSCs in vasculogenic mimicry (VM). Normal human plated on Matrigel were unable form capillary-like structures (CLSs). By contrast, co-cultured with cell lines, namely, Mel Cher, Kor P, generated CLSs. Significantly, poorly cells, Me, failed To identify factors responsible...
A novel cDNA encoding a putative secreted protein was isolated from murine bone marrow. The encoded named MCLP ( urine athelin‐ ike rotein) found to be highly homologous the pig cathelin, and four neutrophil antimicrobial polypeptides: CAP 18, indolicidin, Bac 5 FALL‐39. Secondary structure prediction studies identified cationic region in C‐terminal part of prepro‐MCLP with tendency adopt an amphipathic α‐helical conformation, as observed many peptides. However, no antibacterial activity...