Abstract We studied various aspects of heat‐shock response with special emphasis on the expression protein 70 (hsp70) genes at levels in two congener species littoral endemic amphipods (Eulimnogammarus cyaneus and E. verrucosus) from Lake Baikal which show striking differences their vertical distribution thermal tolerance. Although both demonstrate high constitutive Hsp70, thermotolerant exhibited a 5‐fold higher basal level Hsp70 proteins under normal physiological conditions (7 °C)...

SUMMARY Drosophila melanogaster collected in sub-equatorial Africa the 1970s are remarkably tolerant of sustained laboratory culture above 30°C and acute exposure to much warmer temperatures. Inducible thermotolerance high temperatures, which is due part inducible molecular chaperone Hsp70, only modest this strain. Expression Hsp70 protein hsp70 mRNA likewise reduced has slower kinetics strain (T) than a standard wild-type (Oregon R). These strains also differed constitutive heat-inducible...

Human natural killer (NK) cell differentiation from immature lineage negative (Lin-) umbilical cord blood cells was examined in vitro. Cells expressing antigens of mature NK (CD56, CD16, CD2, CD8, NKR-P1A) were generated Lin- cultured with interleukin (IL)-2 and a murine bone marrow stromal line the human membrane-bound form stem factor. Two subsets identified these cultures: one expressed both NKR-P1A CD56 and, variable proportions, all other antigens; second subset only unlike former, not...

Cytokine-mediated enhancement of spontaneous cytotoxicity depends, at least in part, on modulation the expression surface molecules responsible for recognition target cell structures and triggering or inhibition cytotoxic machinery. We previously demonstrated that transcription factors (e.g., Egr-1, JunB, c-Fos) is differentially regulated by IL-2 IL-12. Here we show CD161/NKR-P1A, a molecule involved cytotoxicity, specifically upregulated CD161 transcription, mRNA accumulation, are...

In this study, we checked whether HSP70 preparations of different origins are able to protect model animals (rats) from endotoxic shock and modify the response myeloid cells lipopolysaccharide (LPS) challenge. It was shown that can effectively organisms by strongly decreasing mortality restoring both homeostasis various hemodynamic characteristics. At cellular level, significantly inhibit LPS-induced reactive oxygen species production in decrease NO expression macrophages, which is enhanced...

Abstract A population of Stratiomys japonica , a species belonging to the family Stratiomyidae (Diptera), common name ‘soldier flies’, occurs in hot volcanic spring, which is apparently among most inhospitable environments for animals because chemical and thermal conditions. Larvae this species, naturally often experience temperatures more than 40 °C, have constitutively high concentrations normally inducible heat‐shock protein Hsp70, but very low level corresponding mRNA. three other same...

Species in the virilis group of Drosophila (fruit flies), which overlap or replace one another along climatic gradients, exhibit corresponding differences basal thermotolerance, inducible thermotolerance and heat-shock response. The low-latitude species D. exceeds high-latitude lummei these measures temperature threshold for factor (HSF) activation ability to express hsp70 mRNA diverse proteins (e.g. Hsp70, Hsp83 small Hsps) after intense heat shock 40-41 degrees C). xeric novamexicana...

ABSTRACT We compare the properties and activation of heat-shock transcription factor (HSF1) synthesis a major family proteins (HSP70) in lizard species inhabiting ecological niches with strikingly different thermal parameters. Under normal non-heat-shock conditions, all desert-dwelling studied so far differ from northern, non-desert (Lacerta vivipara) electrophoretic mobility content constitutively bound to regulatory elements gene promoter. these levels activated HSF1 both HSP70 mRNA...

Abstract Background Previously, we described the heat shock response in dipteran species belonging to family Stratiomyidae that develop thermally and chemically contrasting habitats including highly aggressive ones. Although all studied exhibit high constitutive levels of Hsp70 accompanied by exceptionally thermotolerance, also detected characteristic interspecies differences protein (Hsp) expression survival after severe shock. Here, analyzed genomic libraries from two determined structure...

The heat shock protein 70 (Hsp70, human HSPA1A) plays indispensable roles in cellular stress responses and quality control (PQC). In the framework of PQC, it cooperates with ubiquitin-proteasome system (UPS) to clear damaged dysfunctional proteins cell. Moreover, Hsp70 itself is rapidly degraded following recovery from stress. It was demonstrated that its fast turnover mediated via ubiquitination subsequent degradation by 26S proteasome. At same time, effect on functional state proteasomes...

Abstract Alzheimer’s disease (AD) is a neurodegenerative that inevitably results in dementia and death. Currently, there are no pathogenetically grounded methods for the prevention treatment of AD, all current regimens symptomatic unable to significantly delay development dementia. The accumulation β-amyloid peptide (Aβ), which spontaneous, aggregation-prone, neurotoxic product processing signaling protein APP (Amyloid Precursor Protein), brain tissues, primarily hippocampus frontal cortex,...

In response to heat shock or other metabolic insults, an increased expression of different proteins (hsps) and, in particular, members the hsp70 family is observed human cells. Using two-dimensional electrophoresis, we compared pattern synthesis fibroblast cell lines isolated from two contrasting groups individuals. The first group was represented by native Turk-men living hot desert Middle Asia; second consisted Russians moderate climatic regions European Russia. This analysis has shown...

Although small RNAs efficiently control transposition activity of most transposons in the host genome, such an immune system is not always applicable against a new transposon's invasions. Here, we explored possibility to introduce potentially mobile copy Penelope retroelement previously implicated hybrid dysgenesis syndrome Drosophila virilis into genomes two distant species. The consequences introduction were monitored at different phases after experimental colonization as well D. species,...

Over the last decade, it has become evident that in mammals, including humans, heat shock protein 70 (HSP70), apart from its intracellular localization, is found extracellular space, where may execute various protective functions. Furthermore, upregulation of HSP70 family members can be beneficial prevention and treatment human neurodegenerative diseases cancer. Here, we demonstrate recombinant after intranasal administration penetrate brain regions mice native form subsequently undergo...

Abstract Neuronal dysfunction and loss associated with the accumulation of amyloid-β (Aβ) in form extracellular amyloid plaques hyperphosphorylated tau intraneuronal neurofibrillary tangles represent key features Alzheimer’s disease (AD). Amyloid found brains AD patients are predominantly composed Aβ42 its multiple chemically or structurally modified isoforms. Recently, we demonstrated that isomerised Asp7 (isoAβ42) which is one most abundant Aβ isoform plaques, exhibited high neurotoxicity...

Heat shock proteins including the major stress protein HSP70 support intracellular homeostasis and prevent damage after a temperature increase other stressful environmental stimuli, as well during aging. We have shown earlier that prolonged administration of recombinant human to mice exhibiting Alzheimer's-like neurodegeneration sepsis reduces clinical manifestations these pathologies. Herein, we studied action on young aged mouse mesenchymal stem cells (MSCs) in culture. The results...