A5019260009- Textile materials and evaluations
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Chronic Myeloid Leukemia Treatments
- Animal Genetics and Reproduction
- NF-κB Signaling Pathways
- Chronic Lymphocytic Leukemia Research
- Cell death mechanisms and regulation
- Erythrocyte Function and Pathophysiology
- Genetic and phenotypic traits in livestock
- HER2/EGFR in Cancer Research
- Dyeing and Modifying Textile Fibers
- Ergonomics and Musculoskeletal Disorders
- Genetic Mapping and Diversity in Plants and Animals
- Cytokine Signaling Pathways and Interactions
- Chromosomal and Genetic Variations
- Livestock Management and Performance Improvement
- Lipid Membrane Structure and Behavior
- Cell Adhesion Molecules Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Biosimilars and Bioanalytical Methods
- Myeloproliferative Neoplasms: Diagnosis and Treatment
PSG INSTITUTE OF TECHNOLOGY AND APPLIED RESEARCH
2013-2024
Amgen (United States)
2019-2021
Indian Institute of Technology Madras
1989-2016
Tamil Nadu Veterinary and Animal Sciences University
2015
Human Genome Sciences (United States)
2000-2007
The University of Texas Health Science Center at San Antonio
2007
National Institutes of Health
2007
National Institute of Allergy and Infectious Diseases
2007
University of California, San Diego
2007
Scripps Research Institute
1999
Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) induces apoptosis in a variety of tumour cells through activation TRAIL-R1 and TRAIL-R2 death signalling receptors. Here, we describe the characterisation activity HGS-ETR1, first fully human, agonistic mAb that is being developed as an antitumour therapeutic agent. HGS-ETR1 showed specific binding to receptor. reduced viability multiple types vitro, induced caspase 8, Bid, 9, 3, cleavage PARP, indicating alone was sufficient...
An expression cloning approach was employed to identify the receptor for B-lymphocyte stimulator (BLyS) and identified tumor necrosis factor superfamily member TACI as a BLyS-binding protein. Expression of in HEK293T cells confers on ability bind BLyS with subnanomolar affinity. Furthermore, TACI-Fc fusion protein recognizes both cleaved, soluble form well membrane present cell surface recombinant line. mRNA is found predominantly B-cells correlates binding panel B-cell lines. We also...
Interleukin (IL)-1 is a proinflammatory cytokine with pleiotropic effects in inflammation. IL-1 binding to its receptor triggers cascade of signaling events, including activation the stress-activated mitogen-activated protein (MAP) kinases, c-Jun NH2-terminal kinase (JNK) and p38 MAP kinase, as well transcription factor nuclear kappaB (NF-kappaB). results cellular responses through induction inflammatory gene products such IL-6. One earliest events rapid interaction receptor-associated IRAK...
Interleukin (IL)-18 is functionally similar to IL-12 in mediating T helper cell type 1 (Th1) response and natural killer (NK) activity but related IL-1 protein structure signaling, including recruitment of receptor–associated kinase (IRAK) the receptor activation c-Jun NH2-terminal (JNK) nuclear factor (NF)-κB. The role IRAK IL-18–induced responses was studied IRAK-deficient mice. Significant defects JNK induction partial impairment NF-κB were found Th1 cells, resulting a dramatic decrease...
The primary therapeutic goal for the treatment of diabetes is maintenance a long-term, near-normoglycemic condition and prevention onset or progression complications associated with disease. Although several analogs human insulin have been developed, currently prescribed long-acting do not provide stable basal glycemia more than few hours. Here, we report development Albulin, analog obtained by direct gene fusion single-chain to serum albumin. Albulin showed an elimination t(1/2)...
The transmembrane receptor for immunoglobulin G immune complexes on natural killer (NK) cells and macrophages, Fc gamma RIIIA (CD16), mediates cellular activation through a tyrosine kinase-dependent pathway. We show that RIII crosslinking results in of the src-related kinase p56lck NK demonstrate physical association with immunoprecipitates from obtained using anti-Fc antibodies or complexes. Our studies zeta chain, signal transducing subunit T cell receptor, associates and, cells, is...
The p210bcr/abl tyrosine kinase appears to be responsible for initiating and maintaining the leukemic phenotype in chronic myelogenous leukemia (CML) patients. p21ras-p120GAP interactions play a central role transducing mitogenic signals. Therefore, we investigated whether p21ras p120GAP are regulated by p210bcr/abl, this activation is functionally significant CML cell proliferation. We report that transient expression of fibroblast-like cells induces simultaneous inhibition GTPase-promoting...
Binding of ligand to the alpha subunit Fc gamma RIIIA(CD16), expressed at natural killer (NK) cell membrane in association with homo or heterodimers proteins zeta family, results phosphorylation several on tyrosine residues. We have analyzed role protein regulation molecular events induced upon stimulation RIIIA NK cells and T expressing RIII chain endogenous 2 homodimers devoid other (CD3, CD2) transducing molecules. Our data indicate that treatment these kinase inhibitors prevents not only...
Cytokine-mediated enhancement of spontaneous cytotoxicity depends, at least in part, on modulation the expression surface molecules responsible for recognition target cell structures and triggering or inhibition cytotoxic machinery. We previously demonstrated that transcription factors (e.g., Egr-1, JunB, c-Fos) is differentially regulated by IL-2 IL-12. Here we show CD161/NKR-P1A, a molecule involved cytotoxicity, specifically upregulated CD161 transcription, mRNA accumulation, are...
Induced activation of protein tyrosine kinase(s) is a central event in signal transduction mediated via the low affinity receptor for IgG (Fc gamma RIIIA, CD16) natural killer (NK) cells. Tyrosine phosphorylation may affect function several directly, or indirectly by inducing their association with other phosphorylated proteins. Here, we report that Fc RIII stimulation induces phosphatidylinositol (PI)-3 kinase NK Phosphotyrosine immunoprecipitates from RIII-stimulated cells contain...
Two types of PDGF receptors have been cloned and sequenced. Both are transmembrane glycoproteins with a ligand-stimulatable tyrosine kinase site. We shown earlier that ligand-induced activation the beta-type receptor is due to conversion monomeric form dimeric [Bishayee et al. (1989) J. Biol. Chem. 264, 11699-11705]. In present studies, we established ligand-binding specificity two extended it further investigate association state alpha-receptor role in beta-receptor. These studies were...
Cross-linking the receptors for Fc domain of IgG (Fc gamma R) on leukocytes induces activation protein tyrosine kinases. The intermediary molecules that transduce to nucleus signals leading induction diverse biological responses mediated by these are not clearly identified. We have investigated whether mitogen-activated kinases (MAPK) involved in transmembrane signaling via three R present monocytic, polymorphonuclear, and natural killer (NK) cells. Our results indicate occupancy RI RII...
Despite the clinical success of anti-tumor necrosis factor (TNF) therapies in treatment inflammatory conditions such as rheumatoid arthritis, Crohn disease and psoriasis, full control diseases only occurs a subset patients there is need for new therapeutics with improved efficacy against broader patient populations. One possible approach to combine biological therapeutics, but both cost potential additional toxicities needs be considered. In addition various mediators immune pathways,...
The recognition that few human diseases are thoroughly addressed by mono-specific, monoclonal antibodies (mAbs) continues to drive the development of antibody therapeutics with additional specificities and enhanced activity. Historically, efforts engineer antigen into molecules have relied predominantly on reformatting immunoglobulin domains. In this report we describe a series fully functional mAbs which been imparted through recombinant fusion relatively short polypeptides sequences....
Abstract DcR3/TR6 is a secreted protein belonging to the TNFR family. It binds Fas ligand, LIGHT, and TL1A, all of which are TNF family members. LIGHT expressed on activated T cells. Its known receptors TR2 LTβR cell surface, TR6 in solution. In present study, we report soluble TR6-Fc or solid-phase costimulated proliferation, lymphokine production, cytotoxicity human cells presence TCR ligation. These costimulating effects were blocked by but not Fas-Fc. could also effectively costimulate...
CC chemokine ligand 14, CCL14, is a human that of recent interest because its natural ability, upon proteolytic processing the first eight NH2-terminal residues, to bind and signal through immunodeficiency virus type-1 (HIV-1) co-receptor, receptor 5 (CCR5). We report X-ray crystallographic structures both full-length CCL14 signaling-active, truncated [9−74] determined at 2.23 1.8 Å, respectively. Although differ in their ability CCR5 for biological signaling, we find amino acids (residues 1...