A5062489346- Bone health and treatments
- Mesenchymal stem cell research
- Mast cells and histamine
- Bone Metabolism and Diseases
- MicroRNA in disease regulation
- PI3K/AKT/mTOR signaling in cancer
- Bone Tissue Engineering Materials
- Circular RNAs in diseases
- Cytokine Signaling Pathways and Interactions
- Chronic Myeloid Leukemia Treatments
- Bone and Joint Diseases
- Heterotopic Ossification and Related Conditions
- Tuberous Sclerosis Complex Research
- Cancer-related molecular mechanisms research
- Bone and Dental Protein Studies
- Wound Healing and Treatments
- Virus-based gene therapy research
- dental development and anomalies
- Periodontal Regeneration and Treatments
- Chronic Lymphocytic Leukemia Research
- Endometriosis Research and Treatment
- Bone health and osteoporosis research
- Electrospun Nanofibers in Biomedical Applications
- Medical and Biological Sciences
- Nerve injury and regeneration
Medical University of Warsaw
2015-2024
University of Warsaw
2021
Postgraduate School of Molecular Medicine
1998-2018
Creative Commons
2018
The University of Texas Southwestern Medical Center
2002-2009
Weatherford College
2008
University of Pennsylvania
1996-2007
Academic Center of Medicine
2000-2007
Pfizer (United States)
2006
Pfizer (Canada)
2006
Phosphoinositides have a pivotal role as precursors to important second messengers and asbona fide signaling scaffold targeting molecules. Phosphatidylinositol 4-kinases (PtdIns or PI4Ks) are at the apex of phosphoinsitide cascade. Sequence analysis revealed that mammalian cells contain two type II PtdIns 4-kinase isoforms, now termed PI4KIIα PI4KIIβ. was cloned first. It is tightly membrane-associated behaves an integral membrane protein. In this study, we PI4KIIβ compared isoforms by...
The phenotype of hematopoietic cells transformed by the BCR/ABL oncoprotein Philadelphia chromosome is characterized growth factor-independent proliferation, reduced susceptibility to apoptosis, and altered adhesion motility. mechanisms underlying this are not fully understood, but there evidence that some properties BCR/ABL-expressing dependent on activation downstream effector molecules such as RAS, PI-3k, bcl-2. We show here small GTP-binding protein Rac activated in a tyrosine...
Osteoblastic and chondroblastic (i.e., osteogenic) cells belong to the stromal cell system, which is associated with bone marrow, separate from hematopoietic stem-cell system. Stromal stem are capable of producing reticular, fibroblastic, osteogenic, adipose lines. Marrow-derived osteogenic a component marrow stroma, in vitro form fibroblastic-type colonies. These colonies heterogeneous population varying enzymatic expressions potencies that differentiate into adipocytic, populations. It...
Phosphatidylinositol 4-kinases play essential roles in cell signaling and membrane trafficking. They are divided into type II III families, which have distinct structural enzymatic properties essentially unrelated sequence. Mammalian cells express two isoforms, phosphatidylinositol 4-kinase IIα (PI4KIIα) IIβ (PI4KIIβ). Nearly all of PI4KIIα, about half PI4KIIβ, associates integrally with membranes, requiring detergent for solubilization. This tight association is because palmitoylation a...
BAG3 gene mutations have been recently implicated as a novel cause of dilated cardiomyopathy (DCM). Our aim was to evaluate the prevalence in Polish patients with DCM and search for genotype-phenotype correlations. We studied 90 unrelated probands by direct sequencing exons splice sites. Large deletions/insertions were screened quantitative real time polymerase chain reaction (qPCR). found 5 different 6 total 21 among their relatives: known p.Glu455Lys mutation (2 families), 4 mutations:...
The capacity of bone grafts to repair critical size defects can be greatly enhanced by the delivery mesenchymal stem cells (MSCs). Adipose tissue is considered most effective source MSCs (ADSCs); however, efficiency regeneration using undifferentiated ADSCs low. Therefore, this study proposes scaffolds based on polycaprolactone (PCL), which widely a suitable MSC system, were used as three-dimensional (3D) culture environment promoting osteogenic differentiation ADSCs. PCL enriched with 5%...
Blastic transformation of chronic myelogenous leukemia (CML) is characterized by the presence nonrandom, secondary genetic abnormalities in majority Philadelphia 1 clones, and loss p53 tumor suppressor gene function a consistent finding 25–30% CML blast crisis patients. To test whether functional plays direct role transition phase to crisis, bone marrow cells from +/+ or −/− mice were infected with retrovirus carrying either wild-type BCR/ABL inactive kinase-deficient mutant, assessed for...
Autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) is an autosomal-recessive autoimmune disease caused by regulator gene mutations. The aim of this study was to examine the mutation profile Polish APECED patients, determine carrier rate most frequent mutation(s) and estimate prevalence. While studying 14 unrelated we identified three novel mutations (c.1A>T, affecting start codon; [IVS1 + 1G>C; IVS1 5delG], a complex splice site; c. 908G>C, p.R303P, missense in plant...
Abstract The study examines the preponderance and mechanism of mammalian target rapamycin (mTOR) activation in three distinct types transformed B lymphocytes that differ expression EBV genome. All [EBV-immortalized cells express a broad spectrum virus-encoded genes (type III latency; EBV+/III), EBV-positive only subset EBV-encoded (EBV+/I), EBV-negative, germinal center–derived (EBV−)] universally displayed mTOR signaling pathway. However, EBV+/III also phosphatidylinositol 3-kinase...