A5014099993- Axon Guidance and Neuronal Signaling
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Pain Mechanisms and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Photoreceptor and optogenetics research
- Botulinum Toxin and Related Neurological Disorders
- Memory and Neural Mechanisms
- CRISPR and Genetic Engineering
- Neural dynamics and brain function
- Hereditary Neurological Disorders
- Biochemical effects in animals
- Immune Response and Inflammation
- Neuroendocrine regulation and behavior
- Neurobiology and Insect Physiology Research
- Muscle Physiology and Disorders
- Asthma and respiratory diseases
- Pharmacology and Obesity Treatment
- Transcranial Magnetic Stimulation Studies
- IL-33, ST2, and ILC Pathways
- Viral Infectious Diseases and Gene Expression in Insects
- Genetics and Neurodevelopmental Disorders
- Neurological diseases and metabolism
- Nerve injury and regeneration
- Single-cell and spatial transcriptomics
The Ohio State University Wexner Medical Center
2025
Max Planck Florida Institute for Neuroscience
2017-2021
Laboratory of Molecular Genetics
2019
Osaka University
2007-2016
Japan Science and Technology Agency
2012
Brain injury that results in an initial behavioural deficit is frequently followed by spontaneous recovery. The intrinsic mechanism of this functional recovery has never been fully understood. Here, we show reorganization the corticospinal tract induced target-derived brain-derived neurotrophic factor crucial for motor function following brain injury. After destruction unilateral sensorimotor cortex, intact-side formed sprouting fibres into specific lamina denervated side cervical spinal...
Axon branching is remodeled by sensory-evoked and spontaneous neuronal activity. However, the underlying molecular mechanism largely unknown. Here, we demonstrate that netrin family member netrin-4 (NTN4) contributes to activity-dependent thalamocortical (TC) axon branching. In postnatal developmental stages of rodents, ntn4 expression was abundant in around TC recipient layers sensory cortices. Neuronal activity dramatically altered level cortex vitro vivo. promoted exogenous NTN4...
Target and activity-dependent mechanisms of axonal branching were studied in the thalamocortical (TC) projection using organotypic cocultures thalamus cortex. TC axons labeled with enhanced yellow fluorescent protein (EYFP) by a single-cell electroporation method observed over time confocal microscopy. Changes firing activity grown on multielectrode dishes also monitored time. EYFP-labeled exhibited more branch formation around layer 4 cortical explant during second week vitro , when...
Axonal branching is thought to be regulated not only by genetically defined programs but also neural activity in the developing nervous system. Here we investigated role of pre- and postsynaptic axon thalamocortical (TC) projection using organotypic coculture preparations thalamus cortex. Individual TC axons were labeled with enhanced yellow fluorescent protein transfection into thalamic neurons. To manipulate firing activity, a vector encoding an inward rectifying potassium channel (Kir2.1)...
IgSF11 homophilic cell adhesion molecules regulate the synaptic laminar specificity of cortical chandelier cells.
Different cortical regions processing distinct information, such as the hippocampus and neocortex, share common cellular components circuit motifs but form unique networks by modifying these cardinal units. Cortical circuits include diverse types of GABAergic interneurons (INs) that shape activity excitatory principal neurons (PNs). Canonical IN conserved across have been defined their morphological, electrophysiological, neurochemical properties. However, it remains largely unknown whether...
Neuropathic pain is a chronic condition that occurs frequently after nerve injury and induces hypersensitivity or allodynia characterized by aberrant neuronal excitability in the spinal cord dorsal horn. Fibronectin leucine-rich transmembrane protein 3 (FLRT3) modulator of neurite outgrowth, axon pathfinding, cell adhesion, which upregulated horn following peripheral injury. However, function FLRT3 adults remains unknown. Therefore, we aimed to investigate involvement neuropathic using...
Because of the incomplete understanding molecular mechanisms that underlie chronic pain, currently available treatments for this type pain remain inefficient. In study, we show Netrin-4, a member axon guidance molecule family, was expressed in dorsal horn inner lamina II excitatory interneurons rat spinal cord. A similar expression pattern Netrin-4 also observed human Behavioral analysis revealed tactile and heat hyperalgesia after peripheral nerve injury or inflammation were abolished...
CRISPR/Cas-based technologies have revolutionized genetic approaches to addressing a wide range of neurobiological questions. The ability CRISPR/Cas introduce mutations into target genes allows us perform in vivo loss-of-function experiments without generating genetically engineered mice. However, the lack reliable method determine genotypes individual CRISPR/Cas-transfected cells has made it impossible unambiguously identify cause their phenotypes vivo. Here, we report strategy for...