A5072598157- Axon Guidance and Neuronal Signaling
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neural Engineering
- Nerve injury and regeneration
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Retinal Development and Disorders
- Genomics and Chromatin Dynamics
- Neuroinflammation and Neurodegeneration Mechanisms
- Protein Degradation and Inhibitors
- Angiogenesis and VEGF in Cancer
- Histone Deacetylase Inhibitors Research
- DNA Repair Mechanisms
- Ocular Surface and Contact Lens
- Lung Cancer Treatments and Mutations
- Genetics and Neurodevelopmental Disorders
- 3D Printing in Biomedical Research
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Vestibular and auditory disorders
- Neurobiology and Insect Physiology Research
- Glaucoma and retinal disorders
- Microtubule and mitosis dynamics
- Intraocular Surgery and Lenses
Osaka University
2016-2025
Shenzhen Bay Laboratory
2023-2025
Wakayama Medical University
2016-2024
Creative Commons
2022
Yamamoto Hospital
2022
Ube Frontier University
2006-2015
Chiba Cancer Center
2013
Frontier Science Foundation
2008
The University of Texas Southwestern Medical Center
2004-2005
Southwestern Medical Center
2004
Accumulative evidence indicates that microglial cells influence the normal development of brain synapses. Yet, mechanisms by which these immune target maturating synapses and their functional at early postnatal stages remain poorly understood. Here, we analyzed role CX3CR1, a receptor activated neuronal chemokine CX3CL1 (or fractalkine) controls key functions cells. In whisker-related barrel field mouse somatosensory cortex, show recruitment microglia to sites where developing...
Neural connections were established in cocultures of rat visual cortex (VC) and lateral geniculate nucleus (LGN), which isolated early infancy. Morphological electrophysiological studies showed that the cortical laminar organization afferent efferent coculture preparations was similar to adult VC. The results indicate existence intrinsic mechanisms VC LGN guide formation synaptic with appropriate targets.
Accumulative evidence indicates that microglial cells influence the normal development of central nervous system (CNS) synapses. Yet, functional properties microglia in relation with synapse remain unclear. We recently showed layer 4 whisker-related barrel field mouse somatosensory cortex, are recruited only after postnatal day (P)5 center barrels where thalamo-cortical synapses concentrated and begin their maturation. In present study, we analyzed phenotype during this developmental...
The influence of neuronal activity on cortical axon branching was studied by imaging axons layer 2/3 neurons in organotypic slice cultures rat visual cortex. Upper labeled electroporation plasmid encoding yellow fluorescent protein were observed confocal microscopy. Time-lapse observation single-labeled showed that started to branch after 8-10 d vitro . Over the succeeding 7-10 d, complexity gradually increased both growth and retraction branches, resulting arbors morphologically resembled...
Cortical units were sought that discharged in temporal correlation with spontaneously occurring lens accommodation the area surrounding middle suprasylvian sulcus, between stereotaxic coordinates A8.0 and P1.0, while monitoring by using an infrared optometer. Units tentatively identified as related if their discharges modulated before onset times of accommodation. Forty-eight accommodation-related found. Modulation preceded 360 ms on average. Most (95%) these to increase refractive power...
Transcriptional regulation of gene expression is thought to play a pivotal role in activity-dependent neuronal differentiation and circuit formation. Here, we investigated the histone deacetylase 9 (HDAC9), which regulates transcription by modification, development neocortical neurons. The translocation HDAC9 from nucleus cytoplasm was induced an increase spontaneous firing activity cultured mouse cortical This nucleocytoplasmic also observed postnatal vivo. translocation-induced cellular...
The mammalian neocortex is composed of various types neurons that reflect its laminar and area structures. It has been suggested not only intrinsic but also afferent-derived extrinsic factors are involved in neuronal differentiation during development. However, the role molecular mechanism such almost unknown. Here, we attempted to identify molecules expressed thalamus affect cortical cell First, thalamus-specific were sought by comparing gene expression profiles developing rat cortex using...
Transcriptional regulation is crucial for neuronal activity-dependent processes that govern circuit formation and synaptic plasticity. An intriguing question how activity influences the spatiotemporal interactions between transcription factors their target sites. Here, using a single-molecule imaging technique, we investigated dependence of DNA binding dissociation events cAMP-response element protein (CREB), principal factor in transcription, mouse cortical neurons. To visualize CREB at...
Many cell-intrinsic mechanisms have been shown to regulate neuronal subtype specification in the mammalian neocortex. However, how much cell environment is crucial for determination still remained unclear. Here, we show that knockdown of Protocadherin20 (Pcdh20), which expressed post-migratory neurons layer 4 (L4) lineage, caused cells localize L2/3. The ectopically positioned “future L4 neurons” lost their characteristics but acquired L2/3 characteristics. Knockdown a cytoskeletal protein...
Accumulating evidence indicates that signaling centers controlling the dorsoventral (DV) polarization of neural tube, roof plate and floor plate, play crucial roles in axon guidance along DV axis. However, role regulating rostrocaudal (RC) tube RC axis remains unknown. Here, we show a center located at midbrain-hindbrain boundary (MHB) regulates rostrally directed growth axons from midbrain dopaminergic neurons (mDANs). We found beads soaked with fibroblast factor 8 (FGF8), molecule mediates...
Axon branching is remodeled by sensory-evoked and spontaneous neuronal activity. However, the underlying molecular mechanism largely unknown. Here, we demonstrate that netrin family member netrin-4 (NTN4) contributes to activity-dependent thalamocortical (TC) axon branching. In postnatal developmental stages of rodents, ntn4 expression was abundant in around TC recipient layers sensory cortices. Neuronal activity dramatically altered level cortex vitro vivo. promoted exogenous NTN4...
Abstract Proper spatiotemporal gene expression is achieved by selective DNA binding of transcription factors in the genome. The most intriguing question how dynamic interactions between and their target sites contribute to regulation recruiting basal transcriptional machinery. Here we demonstrate individual dissociation events factor cAMP response element-binding protein (CREB), both vitro living cells, using single-molecule imaging. Fluorescent–tagged CREB bound its sequence cAMP-response...
Neuronal abundance and thickness of each cortical layer are specific to area, but how this fundamental feature arises during development remains poorly understood. While some area-specific features controlled by intrinsic cues such as morphogens transcription factors, the exact influence mechanisms action extrinsic cortex, in particular thalamic axons, have not been fully established. Here, we identify a thalamus-derived factor, VGF, which is indispensable for thalamocortical axons maintain...
The behavior of growing thalamic axons was studied in an organotypic coculture the lateral geniculate nucleus (LGN) with visual cortex (VC) to reveal cellular interactions that underlie formation lamina-specific thalamocortical connections. LGN explant placed at ventral side, pial surface, or edge VC explant, and fluorescent dye-labeled were observed by confocal microscopy fixed living tissue. axonal projection pattern cocultures after 1 week vitro demonstrated that, all three...
Laminar specificity is one of the most striking features neocortical circuitry. To explore molecular basis this specificity, particularly in relation to thalamocortical connectivity, we searched for genes expressed upper cortical layers by constructing a subtraction cDNA library that was enriched layer 4 perinatal rat somatosensory cortex. Differential screening, sequence analysis and situ hybridization demonstrated new unc5 family member (unc5h4), deltex-like gene, stem cell factor (SCF)...
Target and activity-dependent mechanisms of axonal branching were studied in the thalamocortical (TC) projection using organotypic cocultures thalamus cortex. TC axons labeled with enhanced yellow fluorescent protein (EYFP) by a single-cell electroporation method observed over time confocal microscopy. Changes firing activity grown on multielectrode dishes also monitored time. EYFP-labeled exhibited more branch formation around layer 4 cortical explant during second week vitro , when...
Axonal branching is thought to be regulated not only by genetically defined programs but also neural activity in the developing nervous system. Here we investigated role of pre- and postsynaptic axon thalamocortical (TC) projection using organotypic coculture preparations thalamus cortex. Individual TC axons were labeled with enhanced yellow fluorescent protein transfection into thalamic neurons. To manipulate firing activity, a vector encoding an inward rectifying potassium channel (Kir2.1)...
The UNC-5 family of netrin receptors is known to regulate axon guidance, cell migration, and survival. We have previously demonstrated that unc5d, one the member genes, specifically expressed in layer 4 developing rat neocortex (Zhong Y, Takemoto M, Fukuda T, Hattori Murakami F, Nakajima D, Nakayama Yamamoto N. 2004. Identification genes are upper layers neocortex. Cereb Cortex. 14:1144–1152). However, role UNC5D cortical development still unknown. In this study, we revealed unc5d was highly...