A5012128007- Bone Tissue Engineering Materials
- Dental Implant Techniques and Outcomes
- Collagen: Extraction and Characterization
- Hydrogen's biological and therapeutic effects
- biodegradable polymer synthesis and properties
- Anesthesia and Neurotoxicity Research
- Biochemical effects in animals
- Phosphodiesterase function and regulation
- Electrospun Nanofibers in Biomedical Applications
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Periodontal Regeneration and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Pancreatic and Hepatic Oncology Research
- Cancer, Hypoxia, and Metabolism
- Hepatocellular Carcinoma Treatment and Prognosis
- Gastrointestinal disorders and treatments
- Polymer Surface Interaction Studies
- Axon Guidance and Neuronal Signaling
- Pediatric Hepatobiliary Diseases and Treatments
- Epigenetics and DNA Methylation
- Neuroendocrine Tumor Research Advances
- Knee injuries and reconstruction techniques
- Nerve injury and regeneration
- Endoplasmic Reticulum Stress and Disease
Osaka University
2015-2024
National Institute for Materials Science
2004-2024
Psychiatric Medical Center
2024
Kindai University
2024
Ritsumeikan University
2023
Seirei Hamamatsu General Hospital
2016-2022
Osaka Prefectural Medical Center
2022
Fukushima Medical University
2002-2017
Japan Science and Technology Agency
2001-2015
Tokyo Medical and Dental University
2002-2011
Recent studies have suggested that neuronal death in Alzheimer's disease or ischemia could arise from dysfunction of the endoplasmic reticulum (ER). Although caspase-12 has been implicated ER stress-induced apoptosis and amyloid-beta (Abeta)-induced rodents, it is controversial whether similar mechanisms operate humans. We found human caspase-4, a member caspase-1 subfamily includes caspase-12, localized to membrane, cleaved when cells are treated with stress-inducing reagents, but not other...
Chitosan/hydroxyapatite (HAp) composites with a homogeneous nanostructure have been prepared by co-precipitation method. According to TEM observations, HAp crystallites in the formed elliptic aggregations 230 nm length and 50 width. The typical of corresponded approximately that chitosan molecule. size constituent was found be predominantly 30 10 width, c-axes were well aligned parallel molecules respective aggregations. growth is considered occur at nucleation sites, most probably forming...
Alternative sources of mesenchymal stem cells (MSCs) for replacing bone marrow (BM) have been extensively investigated in the field tissue engineering.The purpose this study was to compare osteogenic potential canine MSCs derived from adipose (AT), BM, umbilical cord blood (UCB), and Wharton's jelly (WJ) using vitro culture techniques vivo orthotopic implantation assays.After were isolated various tissues, proliferation along with vascular endothelial growth factor (VEGF) production measured...
Adiponectin, an adipocyte-derived circulating protein, accumulates in vasculature, heart, and skeletal muscles through interaction with a unique glycosylphosphatidylinositol-anchored cadherin, T-cadherin. Recent studies have demonstrated that such accumulation is essential for adiponectin-mediated cardiovascular protection. Here, we demonstrate the adiponectin/T-cadherin system enhances exosome biogenesis secretion, leading to decrease of cellular ceramides. Adiponectin accumulated inside...
Abstract Repeated stressful events are associated with the onset of major depressive disorder (MDD). We previously showed oligodendrocyte (OL)-specific activation serum/glucocorticoid-regulated kinase (SGK)1 cascade, increased expression axon-myelin adhesion molecules and elaboration oligodendrocytic arbor in corpus callosum chronically stressed mice. In current study, we demonstrate that nodes paranodes Ranvier were narrower these Chronic stress also led to diffuse redistribution Caspr Kv...
Demyelination in the central nervous system (CNS) leads to severe neurological deficits that can be partially reversed by spontaneous remyelination. Because CNS is isolated from peripheral milieu blood-brain barrier, remyelination thought controlled microenvironment. However, this work we found factors derived tissue leak into after injury and promote a murine model of toxin-induced demyelination. Mechanistically, leakage circulating fibroblast growth factor 21 (FGF21), which predominantly...
HuD is an RNA-binding protein that has been shown to induce neuronal differentiation by stabilizing labile mRNAs carrying AU-rich instability elements.Here, we show a novel mechanism of arginine methylation coactivator-associated methyltransferase 1 (CARM1) affected mRNA turnover p21 cip1/waf1 in PC12 cells.CARM1 specifically methylated vitro and vivo colocalized with the cytoplasm.Inhibition CARM1 knockdown elongated half-life resulted slow growth rate robust neuritogenesis response nerve...
Repeated stressful events are known to be associated with onset of depression. Further, stress activates the hypothalamic–pituitary–adrenocortical (HPA) system by elevating plasma cortisol levels. However, little is about related downstream molecular pathway. In this study, using repeated water-immersion and restraint (WIRS) as a stressor for mice, we attempted elucidate pathway induced elevated corticosterone We observed following effects both, in vivo vitro: (1) exposure WIRS...
The small GTPase Rab11 plays an important role in the recycling of proteins to plasma membrane as well polarised transport epithelial cells and neurons. We generated conditional knockout mice deficient Rab11a. Rab11a-deficient are embryonic lethal, brain-specific Rab11a show no overt abnormalities brain architecture. In contrast, intestine-specific begin dying approximately 1 week after birth. Apical intestines accumulate cytoplasm mislocalise basolateral membrane, whereas localisation is...
Abstract Of the many serotonin receptors, type 3 receptors (5-HT3R) are only ionotropic ones, playing a key role in fast synaptic transmission and cognitive emotional brain function through controlled neuronal excitation. To better understand various functions of 5-HT3Rs, it is very important to know their expression pattern central nervous system (CNS). date, distributional studies have shown localized 5-HT3R spinal cord. However, an accurate CNS remains be elucidated. investigate...
A hydroxyapatite/type I collagen (HAp/Col) composite, in which the hydroxyapatite nanocrystals align along molecules, has been prepared. The biocompatibility, osteoconductive activity, and efficacy as a carrier of recombinant human bone morphogenetic proteins (rhBMPs) this novel biomaterial were examined. composite material was implanted backs Wistar rats, specimens collected for histological observations until week 24. In second experiment, other samples (5 × 5 10 mm3) drilled immersed...
Application of differential display to cultured rat astrocytes allowed cloning Herp cDNA. Although was strongly induced by endoplasmic reticulum (ER) stress, it decayed rapidly consequent proteasome-mediated degradation. To investigate the role this molecule in terms stress response, knockout cells were developed using F9 embryonic carcinoma cells. null more vulnerable ER compared with wild-type In early period (0-8 h after tunicamycin treatment), displayed enhanced signalling and...
Novel composites of bioactive beta-tricalcium phosphate [Ca(3)(PO(4))(2)] and biodegradable copolymerized poly-L-lactide (CPLA) were prepared by a heat-kneading method. The mechanical chemical changes the evaluated in vitro soaking physiological saline Dulbecco's buffered saline. When soaked saline, 3-point strength decreased rapidly from 60 to 30 MPa initial 4 weeks then gradually reached plateau; decrease was ascribed dissolution surface. properties evident at 8-12 sufficient for be used...
Disrupted-in-schizophrenia 1 (DISC1) is a gene disrupted by translocation, t(1;11) (q42.1;q14.3), that segregates with major psychiatric disorders, including schizophrenia, recurrent depression and bipolar affective disorder, in Scottish family. Here we report mammalian DISC1 endogenously expressed oligodendroglial lineage cells negatively regulates differentiation of oligodendrocyte precursor into oligodendrocytes. expression was detected oligodendrocytes the mouse corpus callosum at P14...
Although a causal relationship between inflammation and innate immunity of cancer is more widely accepted today, many the precise cell mechanisms mediating this have not been elucidated. Th17 cells, which produce proinflammatory cytokine interleukin 17 (IL‑17), recognized as one key factors in regulation inflammatory bowel disease rheumatoid arthritis. This study demonstrated that, patients with various types gastrointestinal cancer, IL‑17 production was correlated myeloid‑derived suppressor...
Pericytes play pivotal roles in physiological and pathophysiological conditions the central nervous system. As pericytes prevent vascular leakage, they can halt neuronal damage stemming from a compromised blood-brain barrier. Therefore, may be good target for treatment of neurodegenerative disorders, although evidence is lacking. In this study, we show that prostacyclin attenuates lysophosphatidylcholine (LPC)-mediated dysfunction through pericyte protection adult mouse spinal cord. LPC...
The Golgi apparatus is important for the transport of secretory cargo. Glycosylation a major post-translational event. Recognition O-glycans on proteins necessary glycoprotein trafficking. In this study, specific inhibition O-glycosylation (Golgi stress) induced expression endoplasmic reticulum (ER)-resident heat shock protein (HSP) 47 in NIH3T3 cells, although cell death was not by stress alone. When HSP47 downregulated siRNA, caused death. Three days after induction stress, disassembled,...
This study was performed to evaluate the osteogenic effect of allogenic canine umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) mixed with beta-tricalcium phosphate (beta-TCP) in orthotopic implantation. Seven hundred milligrams beta-TCP 1 x 10(6) UCB-MSCs diluted 0.5 ml saline (group CM) and same volume as control C) were implanted into a 1.5 cm diaphyseal defect wrapped PLGC membrane radius Beagle dogs. Radiographs antebrachium made after surgery. The implants harvested 12...
The effect of low reactive-level laser therapy (LLLT) with a He-Ne on operative wound healing was investigated in rat model. 10-millimeter surgical wounds were created the backs Sprague Dawley rats, and animals assigned to one eleven groups (n=5). Ten received either 8.5 mW or 17.0 irradiation 15 seconds LLLT day five different frequencies, i.e. daily (from 1st 6th following surgery), every other (the 1st, 3rd, 5th day), only day, 3rd day; surgery. control group no irradiation. A skin...