A5001233025- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Retinal Development and Disorders
- Advanced Electron Microscopy Techniques and Applications
- Neural dynamics and brain function
- Advanced Fluorescence Microscopy Techniques
- Ion channel regulation and function
- Cellular transport and secretion
- Cell Image Analysis Techniques
- Neurobiology and Insect Physiology Research
- Mitochondrial Function and Pathology
- Receptor Mechanisms and Signaling
- Bacterial biofilms and quorum sensing
- Alzheimer's disease research and treatments
- Molecular Biology Techniques and Applications
- Hereditary Neurological Disorders
- Electron and X-Ray Spectroscopy Techniques
- Parkinson's Disease Mechanisms and Treatments
- Marine Biology and Ecology Research
- Endoplasmic Reticulum Stress and Disease
- bioluminescence and chemiluminescence research
- Advanced Memory and Neural Computing
- Ocean Acidification Effects and Responses
- Biotin and Related Studies
Max Planck Florida Institute for Neuroscience
2014-2024
Imaging Center
2020-2022
Significance Parkinson’s disease (PD) is a neurodegenerative linked to the misfolding and aggregation of protein called “α-synuclein.” α-Synuclein aggregates found in brains PD patients are “Lewy bodies” neurites.” We discovered existence type α-synuclein aggregate, smaller than previously described conformationally distinct, that we “pα-syn*.” Pα-syn* was present neuronal cultures mice injected with recombinant fibrils as well patients. showed pα-syn* made trimmed resulting from failed...
In the presynaptic terminal, magnitude and location of Ca2+ entry through voltage-gated channels (VGCCs) regulate efficacy neurotransmitter release. However, how active zone proteins control mammalian VGCC levels organization is unclear. To address this, we deleted CAST/ELKS protein family at calyx Held, a CaV2.1 channel-exclusive terminal. We found that loss reduces current density with concomitant reductions in channel numbers clusters. Surprisingly, deletion increases release probability...
The calyx of Held, a large glutamatergic presynaptic terminal in the auditory brainstem undergoes developmental changes to support high action-potential firing rates required for information encoding. In addition, terminals are morphologically diverse, which impacts vesicle release properties and synaptic plasticity. Mitochondria influence plasticity through calcium buffering crucial providing energy transmission. Therefore, it has been postulated that mitochondrial levels increase during...
Non-typeable Haemophilus influenzae (NTHi) is a common acute otitis media pathogen, with an incidence that increased by previous antibiotic treatment. NTHi also emerging causative agent of other chronic infections in humans, some linked to morbidity, and all which impose substantial treatment costs. In this study we explore the possibility exposure may stimulate biofilm formation bacteria. We discovered sub-inhibitory concentrations beta-lactam (i.e., amounts partially inhibit bacterial...
Electron microscopy (EM) enables high-resolution visualization of protein distributions in biological tissues. For detection, gold nanoparticles are typically used as an electron-dense marker for immunohistochemically labeled proteins. Manual annotation particle labels is laborious and time consuming, counts can exceed 100,000 across hundreds image segments to obtain conclusive data sets. To automate this process, we developed Gold Digger, a software tool that uses modified pix2pix deep...
Synapses contain a limited number of synaptic vesicles (SVs) that are released in response to action potentials (APs). Therefore, sustaining transmission over wide range AP firing rates and timescales depends on SV release replenishment. Although actin dynamics impact transmission, how presynaptic regulators signaling cascades control replenishment remains unresolved. Rac1, Rho GTPase, regulates synaptogenesis, neuronal development, postsynaptic function. However, the role Rac1 regulating is...
Purpose: Mutations in LRIT3 lead to complete congenital stationary night blindness (cCSNB). Using a cCSNB mouse model lacking Lrit3 (nob6), we recently have shown that has role the correct localization of TRPM1 (transient receptor potential melastatin 1) dendritic tips ON-bipolar cells (BCs), contacting both rod and cone photoreceptors. Furthermore, postsynaptic clustering other mGluR6 cascade components is selectively eliminated at ON-BCs. The purpose this study was further define...
Abstract
CAST/ELKS are positive regulators of presynaptic growth and suppressors active zone expansion at the developing mouse calyx Held. regulate all three CaV 2 subtype channel levels in terminal not just 2.1. The half-life ELKS is on timescale days weeks. Synaptic transmission was impacted by loss CAST/ELKS. involved pathways regulating morphological properties terminals during an early stage circuit maturation.Many (AZ) proteins have multiple regulatory roles that vary distinct stages neuronal...
IgSF11 homophilic cell adhesion molecules regulate the synaptic laminar specificity of cortical chandelier cells.
ADAP1/Centaurin-α1 (CentA1) functions as an Arf6 GTPase-activating protein highly enriched in the brain. Previous studies demonstrated involvement of CentA1 brain function a regulator dendritic differentiation and potential mediator Alzheimer's disease (AD) pathogenesis. To better understand neurobiological signaling brain, we developed Centa1 knock-out (KO) mice. The KO animals showed neither development nor synaptic ultrastructure deficits hippocampus. However, they exhibited significantly...
Abstract Variations in utricle morphology may be responsible for different tolerances to ocean acidification (OA) within the macroalgal genus Halimeda , an important sediment producer on reefs. However, differences species’ and their relationship calcification crystal formation have not been well articulated. In present study, we characterized morphologies of six species. Primary ultrastructure was quantitatively qualitatively compared tissue inorganic content microstructure. Morphologies...
The overwhelming majority of bacteria live in slime embedded microbial communities termed biofilms, which are typically adherent to a surface. However, when several Staphylococcus epidermidis strains were cultivated static liquid cultures, macroscopic aggregates seen floating within the broth and also sedimented at test tube bottom. Light- electron microscopy revealed that early-stage consisted extracellular matrix, organized sheet-like structures. Perpendicular under sheets hung network...
Significance This study addresses a fundamental question in neuroscience: how do neurons functionally specify and diversify their synaptic connections? is particularly relevant the visual system which handle broad range of stimuli requiring synapses to adjust gain accordingly. In this work, we describe cone photoreceptors, all vertebrate animals utilize for daylight vision, task through an elegant trans-synaptic mechanism involving splice isoform cell-adhesion molecule, Latrophillin 3.
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Integral membrane proteins such as ion channels, transporters, and receptors shape cell activity mediate cell-to-cell communication in the brain. The distribution, quantity, clustering arrangement of those contribute to physiological properties cell; therefore, precise quantification their state can be used gain insight into cellular function. Using a highly sensitive immunoelectron microscopy technique called sodium dodecyl sulfate-digested freeze-fracture replica immunogold labeling...
PMP22 and MPZ are abundant myelin membrane proteins in Schwann cells. The adhesion protein holds wraps together across the intraperiod line. is a tetraspan belonging to Claudin superfamily. Loss of either or causes severe demyelinating Charcot-Marie-Tooth (CMT) peripheral neuropathy, duplication most common form CMT, CMT1A. Yet, molecular functions provided by how its alteration CMT unknown. Here we find specific complex through interfaces within their transmembrane domains. We also that...
Abstract Many presynaptic active zone proteins have multiple regulatory roles that vary during distinct stages of neuronal circuit development. However, our understanding how regulate synapse development maturation is in its early stages. Although CAST/ELKS are core proteins, their the mammalian central nervous system remain enigmatic. To unravel glutamatergic development, we analyzed loss impacted calyx Held terminal and function. We found a reduction surface area volume, but an increase...
Abstract Neuroligin-1 (NLGN1) is a cell adhesion molecule found at excitatory glutamatergic synapses in the brain which regulates synaptic function and maturation. Extracellular cleavage of NLGN1 by proteases has been shown to control vesicle release cultured neurons, but nothing known about underlying changes synapse structure that accompany this, or how affected tissue. We prevention through mutation extracellular stalk domain increases docking miniature post-synaptic current frequency...
Abstract The calyx of Held, a large glutamatergic presynaptic terminal in the auditory brainstem undergoes developmental changes to support high action-potential firing rates required for information encoding. In addition, terminals are morphologically diverse which impacts vesicle release properties and synaptic plasticity. Mitochondria influence plasticity through calcium buffering crucial providing energy transmission. Therefore, it has been postulated that mitochondrial levels increase...