A5057471497- Cellular transport and secretion
- Fungal and yeast genetics research
- Bioinformatics and Genomic Networks
- Hereditary Neurological Disorders
- Protein Kinase Regulation and GTPase Signaling
- Gene expression and cancer classification
- Cellular Mechanics and Interactions
- RNA regulation and disease
- Biotin and Related Studies
- Calcium signaling and nucleotide metabolism
- RNA Research and Splicing
- Neurological diseases and metabolism
- Wnt/β-catenin signaling in development and cancer
- Lipid Membrane Structure and Behavior
- Erythrocyte Function and Pathophysiology
- Calpain Protease Function and Regulation
- Heat shock proteins research
- Neuroscience and Neuropharmacology Research
- Plant nutrient uptake and metabolism
- Botulinum Toxin and Related Neurological Disorders
- Neurogenetic and Muscular Disorders Research
- Microbial Natural Products and Biosynthesis
- Lysosomal Storage Disorders Research
- Melanoma and MAPK Pathways
- ATP Synthase and ATPases Research
University of Iowa
2012-2024
New Mexico State University
2008-2012
The synaptotagmin (syt) proteins have been widely studied for their role in regulating fusion of intracellular vesicles with the plasma membrane. Here we report that syt-17, an unusual isoform unknown function, plays no exocytosis, and instead multiple roles membrane trafficking. Syt-17 is localized to Golgi complex hippocampal neurons, where it coordinates import from endoplasmic reticulum support neurite outgrowth facilitate axon regrowth after injury. Further, discovered a second pool...
Abstract Mutations in MPZ (myelin protein zero) can cause demyelinating early-onset Charcot-Marie-Tooth type 1B disease or later onset 2I/J characterized by axonal degeneration, reflecting the diverse roles of Schwann cells. holds apposing membranes myelin sheath together, with adhesion role fulfilled its extracellular immunoglobulin-like domain (IgMPZ), which oligomerizes. Models for how IgMPZ might form oligomeric assemblies has been extrapolated from a crystal structure individual rat...
Abstract Grb7 is an adaptor molecule that can mediate signal transduction from multiple cell surface receptors to various downstream signaling pathways. Grb7, along with Grb10 and Grb14, make up the protein family. This family has been shown be overexpressed in certain cancers cancer lines. a receptor tyrosine kinase (RTK), erbB2, are 20–30% of breast cancers. overexpression linked enhanced migration metastasis, though participants these pathways have not determined. In this study, we report...
We describe a regulatory mechanism that controls the activity of retromer, an evolutionarily conserved sorting device orchestrates cargo export from endosome. A spontaneously arising mutation activates yeast (Saccharomyces cerevisiae) CDC25 family phosphatase, Mih1, results in accelerated turnover subset endocytosed plasma membrane proteins due to deficient into retromer-mediated recycling pathway. Mih1 directly modulates phosphorylation state Vps26 retromer subunit; mutations engineered...
Adaptor proteins mediate signal transduction from cell surface receptors to downstream signaling pathways. The Grb7 protein family of adaptor is constituted by Grb7, Grb10, and Grb14. This has been shown be overexpressed in certain cancers cancer lines. Grb7-mediated migration proceed through a focal adhesion kinase (FAK)/Grb7 pathway, although the specific participants have not fully determined. In this study, we report that interacts with Hax-1, cytoskeletal-associated found metastatic...
In previous studies, we showed that the tyrosine phosphorylation state of growth factor receptor-bound protein 7 (Grb7) affects its ability to bind transcription regulator FHL2 and cortactin-interacting protein, human HS-1-associated protein-1. Here, present results describing importance dimerization in Grb7-Src homology 2 (SH2) domain terms structural integrity phosphorylated peptide ligands. A phosphorylation-mimic mutant (Y80E-Grb7-SH2) is largely deficient binds a tyrosine-phosphorylated...
Screening for protein-protein interactions using the yeast 2-hybrid assay has long been an effective tool, but its use largely limited to discovery of high-affinity interactors that are highly enriched in library interacting candidates. In a traditional format, can yield too many colonies analyze when conducted at low stringency where affinity might be found. Moreover, without comprehensive and complete interrogation same against different bait plasmids, comparative analysis cannot achieved....
PMP22 and MPZ are abundant myelin membrane proteins in Schwann cells. The adhesion protein holds wraps together across the intraperiod line. is a tetraspan belonging to Claudin superfamily. Loss of either or causes severe demyelinating Charcot-Marie-Tooth (CMT) peripheral neuropathy, duplication most common form CMT, CMT1A. Yet, molecular functions provided by how its alteration CMT unknown. Here we find specific complex through interfaces within their transmembrane domains. We also that...
We have adapted the yeast 2-hybrid assay to simultaneously uncover dozens of transient and static protein interactions within a single screen utilizing high-throughput short-read DNA sequencing. The resulting sequence datasets can not only track what genes in population that are enriched during selection for positive interactions, but also give detailed information about relevant subdomains proteins sufficient interaction. Here, we describe full suite stand-alone software programs allow...
Screening for protein-protein interactions using the yeast 2-hybrid assay has long been an effective tool, but its use largely limited to discovery of high-affinity interactors that are highly enriched in library interacting candidates.In a traditional format, 2hybrid can yield too many colonies analyze when conducted at low stringency where affinity might be found.Moreover, without comprehensive and complete interrogation same against different bait plasmids, comparative analysis cannot...
ABSTRACT Mutations in Myelin Protein Zero (MPZ) account for 5% of Charcot-Marie-Tooth cases and can cause demyelinating or axonal phenotypes, reflecting the diverse roles MPZ Schwann cells. holds apposing membranes myelin sheath together, with adhesion role fulfilled by extracellular lmmunoglobulin-like domain (lg ), which oligomerize. Current knowledge how lg might form oligomeric assemblies involving 3 weakly-interacting interfaces has been extrapolated from a protein crystal structure...
The family of Ras‐related small molecular weight GTP‐binding proteins encompasses over 160 members. Together, these members control myriad aspects cell biological processes by binding effector in a manner that is dependent on whether they are bound to GTP or GDP. Ras‐family GTPases built conserved structural template dictates the mode nucleotide binding. This provides opportunity create mutations within residues force protein into GDP‐bound conformation, which typically inactive, GTP‐bound...
We have adapted the yeast 2-hybrid assay to simultaneously uncover dozens of transient and static protein interactions within a single screen utilizing high-throughput short-read DNA sequencing. The resulting sequence datasets can not only track what genes in population that are enriched during selection for positive interactions, but also give detailed information about relevant subdomains proteins sufficient interaction. Here, we describe full suite stand-alone software programs allow...