A5038845656- Biochemical and Molecular Research
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Lipid Membrane Structure and Behavior
- Porphyrin Metabolism and Disorders
- Enzyme Structure and Function
- HIV/AIDS drug development and treatment
- Force Microscopy Techniques and Applications
- Polymer Surface Interaction Studies
- Mass Spectrometry Techniques and Applications
- Protein Kinase Regulation and GTPase Signaling
- Virus-based gene therapy research
- Influenza Virus Research Studies
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Cellular Mechanics and Interactions
- Bacterial Genetics and Biotechnology
- Genetics, Bioinformatics, and Biomedical Research
- Pharmacological Receptor Mechanisms and Effects
- Electrostatics and Colloid Interactions
- Adenosine and Purinergic Signaling
- Trypanosoma species research and implications
- Supramolecular Self-Assembly in Materials
- Protein Structure and Dynamics
University of Arizona
2010-2022
New Mexico State University
2012
University of California, Santa Barbara
1997-2001
University of Wuppertal
2000
Boston University
1999
Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules their complexes, reporting on size shape in free solution. The purpose this multi-laboratory study was establish the precision accuracy basic data dimensions AUC validate previously proposed calibration techniques. Three kits cell assemblies containing radial temperature tools bovine serum albumin (BSA) reference sample were...
During cellular invasion, human papillomavirus type 16 (HPV16) must transfer its viral genome (vDNA) across the endosomal membrane prior to accumulation at nuclear PML bodies for establishment of infection. After uptake, capsid likely undergoes pH-dependent disassembly within endo-/lysosomal compartment, thereby exposing hidden domains in L2 that facilitate penetration L2/vDNA complexes. In an effort identify regions might physically interact with membranes, we have subjected sequence...
Viroporins are small viral ion channels that play important roles in the infection cycle and proven antiviral drug targets. Matrix protein 2 from influenza A (AM2) is best-characterized viroporin, current paradigm AM2 forms monodisperse tetramers. Here, we used native mass spectrometry other techniques to characterize oligomeric state of both full-length transmembrane (TM) domain a variety different pH detergent conditions. Unexpectedly, discovered formed range complexes were strongly...
ADVERTISEMENT RETURN TO ISSUEPREVNoteFormation of Tethered Supported Bilayers by Vesicle Fusion onto Lipopolymer Monolayers Promoted Osmotic StressMarkus Seitz, Evgeny Ter-Ovanesyan, Marcus Hausch, Chad K. Park, Joseph A. Zasadzinski, Rudolf Zentel, and Jacob N. IsraelachviliView Author Information Department Chemical Engineering, University California, Santa Barbara, California 93106, BUGH Wuppertal, Fachbereich Chemie und Institut für Materialwissenschaften, Gauss Strasse 20, D-42097...
Enkephalin analogues with a 4-anilidopiperidine scaffold have been designed and synthesized to achieve therapeutic benefit for the treatment of pain due mixed μ δ opioid agonist activities. Ligand 16, in which Dmt-substituted enkephalin-like structure was linked N-phenyl-N-piperidin-4-yl propionamide moiety, showed very high binding affinities (0.4 nM) at receptors an increased hydrophobicity (aLogP = 2.96). This novel lead compound found potent activities MVD (1.8 GPI (8.5 assays.
SgrAI is a type IIF restriction endonuclease that cuts an unusually long recognition sequence and exhibits self-modulation of DNA cleavage activity specificity. Previous studies have shown forms large oligomers when bound to particular sequences under the same conditions where accelerated kinetics. However, detailed structure stoichiometry SgrAI–DNA complex as well basic building block not been fully characterized. Ion mobility mass spectrometry (IM-MS) was employed analyze complexes show...
SgrAI is a type II restriction endonuclease that cuts an unusually long recognition sequence and exhibits allosteric self-modulation of DNA activity specificity. Precleaved primary site has been shown to be effector [Hingorani-Varma, K., Bitinaite, J. (2003) Biol. Chem. 278, 40392−40399], stimulating cleavage both (CR|CCGGYG, where the vertical bar indicates cut site, R denotes A or G, Y C T) secondary [CR|CCGGY(A/C/T) CR|CCGGGG] sequences. The fact this suggests at least two binding sites...
SgrAI is a type IIF restriction endonuclease that cuts an unusually long recognition sequence and exhibits allosteric self-modulation of cleavage activity specificity. Previous studies have shown DNA bound dimers oligomerize into activated form with higher rates, although previously determined crystal structures to show only the dimer. A new structure II Ca2+ now presented, which shows close association two dimers. This tetrameric unlike those homologous enzymes Cfr10I NgoMIV formed by...
SgrAI is a type II restriction endonuclease with an unusual mechanism of activation involving run-on oligomerization. The oligomer formed from complexes bound to DNA containing its 8 bp primary recognition sequence (uncleaved or cleaved), and also binds (and thereby activates for cleavage) secondary site sequences which contain single base substitution in either the 1st/8th 2nd/7th position sequence. This modulation enzyme activity via oligomerization newly appreciated phenomenon that has...
Much of the short-range forces and structures softly supported DMPC bilayers has been described previously. However, one interesting feature measured force-distance profile that remained unexplained is presence a long-range exponentially decaying repulsive force not observed between rigidly on solid mica substrate surfaces. This observation discussed in detail here based recent static dynamic surface experiments. The intermediate distance regime (mica-mica separations from 15 to 40 nm) are...
This work is motivated by an interest in understanding the characteristics and advantages of a relatively newly discovered enzyme mechanism involving filament formation. SgrAI responsible for protecting against viral infections its host bacterium was one first such enzymes shown to utilize mechanism. In this work, formation disrupted, effects on speed purified as well function cells are measured. It found that even small disruptions, which weaken but do not destroy formation, eliminate...
In previous studies, we showed that the tyrosine phosphorylation state of growth factor receptor-bound protein 7 (Grb7) affects its ability to bind transcription regulator FHL2 and cortactin-interacting protein, human HS-1-associated protein-1. Here, present results describing importance dimerization in Grb7-Src homology 2 (SH2) domain terms structural integrity phosphorylated peptide ligands. A phosphorylation-mimic mutant (Y80E-Grb7-SH2) is largely deficient binds a tyrosine-phosphorylated...
Protein-based self-assembled nanostructures hold tremendous promise as smart materials. One strategy to control the assembly of individual protein modules takes advantage directionality and high affinity bonding afforded by metal chelation. Here, we describe use 2,2'-bipyridine units (Bpy) side chains template large structures (MW approx. 35 000 Da) in a metal-dependent manner. The are trimers independently folded 3-helix bundles, held together 2 Me(Bpy)3 complexes. assemblies stable thermal...
Abstract It is well known the dimerization state of receptor tyrosine kinases (RTKs), in conjunction with binding partners such as growth factor bound protein 7 (Grb7) protein, plays an important role cell signaling regulation. Previously, we proposed, downstream RTKs, that phosphorylation Grb7SH2 domain residues could control Grb7 dimerization, and may be regulatory step to RTKs. In this manner, additional dimerization‐dependent regulation occur membrane‐bound kinase RTK‐mediated pathways....
Abstract Viroporins are small viral ion channels that play important roles in the infection cycle and proven antiviral drug targets. Matrix protein 2 from influenza A (AM2) is best characterized viroporin, current paradigm AM2 forms monodisperse tetramers. Here, we used native mass spectrometry other techniques to characterize oligomeric state of both full-length transmembrane domain (TM) a variety different pH detergent conditions. Unexpectedly, discovered formed range complexes were...