A5024082079- Biochemical and Molecular Research
- Porphyrin Metabolism and Disorders
- Adenosine and Purinergic Signaling
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Cytomegalovirus and herpesvirus research
- Neonatal Health and Biochemistry
- Microbial metabolism and enzyme function
- Enzyme Structure and Function
- RNA Research and Splicing
- Microbial Metabolites in Food Biotechnology
- Glycosylation and Glycoproteins Research
- Blood properties and coagulation
- Chemical Synthesis and Analysis
- Veterinary medicine and infectious diseases
- Hemoglobin structure and function
- Helicobacter pylori-related gastroenterology studies
- Heat shock proteins research
- Leptospirosis research and findings
- Biosimilars and Bioanalytical Methods
- Protein purification and stability
- PI3K/AKT/mTOR signaling in cancer
- Polyamine Metabolism and Applications
- Supramolecular Self-Assembly in Materials
University of Warsaw
2006-2023
Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules their complexes, reporting on size shape in free solution. The purpose this multi-laboratory study was establish the precision accuracy basic data dimensions AUC validate previously proposed calibration techniques. Three kits cell assemblies containing radial temperature tools bovine serum albumin (BSA) reference sample were...
The protease high temperature requirement A from the gastric pathogen Helicobacter pylori (HtrA Hp ) belongs to well conserved family of serine proteases. HtrA is an important secreted virulence factor involved in disruption tight and adherens junctions during infection. Very little known about function H. cell physiology due lack htrA knockout strains. Here, using a newly constructed ΔhtrA mutant strain, we found that bacteria deprived showed increased sensitivity certain types stress,...
Abstract— We examined the emission spectra of tyrosine‐ and tryptophan‐containing proteins using one‐photon (270–310 nm) two‐photon (565–610 excitation. Emission for excitation native denatured human serum albumin three purine nucleoside phosphorylases indicated an absence tyrosine normally seen below 290 nm. tyrosine‐tryptophan mixtures to determine origin selective tryptophan residues. These results confirmed a short‐wavelength shift spectrum relative that tryptophan, as recently reported...
Leptospira interrogans is a spirochaete responsible for leptospirosis in mammals. The molecular mechanisms of the virulence remain mostly unknown. Recently, it has been demonstrated that an AAA+ chaperone ClpB (a member Hsp100 family) from L. (ClpBLi) not only essential survival under thermal and oxidative stresses, but also during infection host. aim this study was to provide further insight into role pathogenic spirochaetes explore its biochemical properties. We found non-hydrolysable ATP...
Initiation is the rate-limiting step during mRNA 5' cap-dependent translation, and thus a target of strict control in eukaryotic cell. It shown here by analytical ultracentrifugation fluorescence spectroscopy that affinity human translation inhibitor, eIF4E-binding protein (4E-BP1), to initiation factor 4E significantly higher when eIF4E bound cap. The 4E-BP1 binding stabilizes active conformation and, on other hand, can facilitate dissociation from These findings reveal particular...
The incorporation of cyclopentane-based beta-amino acid in the sequence peptide forming coiled-coil induced formation nanofibrils.
Molecular mechanisms underlying the recognition of mRNA 5′ terminal structure called “cap” by eukaryotic initiation factor 4E (eIF4E) are crucial for cap-dependent translation. To gain a deeper insight into how yeast eIF4E interacts with cap structure, isothermal titration calorimetry and van’t Hoff analysis based on intrinsic protein fluorescence quenching upon series chemical analogs were performed, providing consistent thermodynamic description binding process in solution. Equilibrium...
Purine nucleoside phosphorylase (PNP) is a key enzyme of the salvage pathway and characterized by complex kinetics. It was suggested that this due to coexistence various oligomeric forms differ in specific activity. In work, molecular architecture Escherichia coli PNP solution studied analytical ultracentrifugation CD spectroscopy. Sedimentation equilibrium analysis revealed homohexameric molecule with mass 150+/-10 kDa, regardless conditions investigated-protein concentration, 0.18-1.7...
The cap at the 5'terminus of mRNA is a key determinant gene expression in eukaryotic cells, which among others required for dependent translation and protects from degradation. These properties are mediated by several proteins. One them 4E-Transporter (4E-T), plays an important role translational repression, decay P-bodies formation. 4E-T also one proteins that interact with initiation factor 4E (eIF4E), binding protein component machinery. molecular mechanisms underlying interactions these...
Purine nucleoside phosphorylase (PNP) is a ubiquitous enzyme of the salvage pathway and it characterized by non-Michaelis kinetics. Kinetic data in many cases (for some substrates or concentration range co-substrate) are best described double hyperbolic equation. It was suggested that this an indication being example morpheein, i.e. protein exists as equilibrium quaternary structure isoforms. In paper we summarize our already published for calf spleen E. coli PNPs, well new experiments...