A5025213293- Bacterial Genetics and Biotechnology
- Helicobacter pylori-related gastroenterology studies
- Antibiotic Resistance in Bacteria
- DNA Repair Mechanisms
- Microbial Metabolites in Food Biotechnology
- Bacteriophages and microbial interactions
- Galectins and Cancer Biology
- Salmonella and Campylobacter epidemiology
- Probiotics and Fermented Foods
- RNA and protein synthesis mechanisms
- Trace Elements in Health
- Genomics and Phylogenetic Studies
- Clostridium difficile and Clostridium perfringens research
- X-ray Diffraction in Crystallography
- Escherichia coli research studies
- Mycobacterium research and diagnosis
- RNA modifications and cancer
- Crystallization and Solubility Studies
- Viral gastroenteritis research and epidemiology
- Gut microbiota and health
- Gastrointestinal disorders and treatments
- Listeria monocytogenes in Food Safety
- Antimicrobial Resistance in Staphylococcus
- Metal-Organic Frameworks: Synthesis and Applications
- Culinary Culture and Tourism
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
2015-2024
Polish Academy of Sciences
2015-2024
University of Wrocław
2012-2014
Institute of Immunology
2014
HTW Berlin - University of Applied Sciences
2012
Max Planck Institute for Molecular Genetics
2012
Institut Pasteur
2007
The protease high temperature requirement A from the gastric pathogen Helicobacter pylori (HtrA Hp ) belongs to well conserved family of serine proteases. HtrA is an important secreted virulence factor involved in disruption tight and adherens junctions during infection. Very little known about function H. cell physiology due lack htrA knockout strains. Here, using a newly constructed ΔhtrA mutant strain, we found that bacteria deprived showed increased sensitivity certain types stress,...
Bacterial chromosomes (though not Escherichia coli and some other γ-proteobacterial chromosomes) contain parS sequences parAB genes encoding partitioning proteins, i.e. ParA (ATPase) ParB (DNA-binding proteins) that are components of the segregation machinery. Here, mycobacterial parABS elements were characterized for first time. essential in Mycobacterium smegmatis; however, elimination or overexpression protein causes growth inhibition. Deletion parB also leads to a rather severe...
Bacterial DNA replication requires DnaA, an AAA+ ATPase that initiates at a specific chromosome region, oriC, and is regulated by species-specific regulators directly bind DnaA. HobA DnaA binding protein, recently identified as essential regulator of in Helicobacter pylori. We report the crystal structure complex with domains I II (DnaA(I-II)) from H. pylori, first bound to one its regulators. Biochemical characterization formed shows tetramer binds four DnaA(I-II) molecules, unable...
Binding of the DnaA protein to oriC leads DNA melting within unwinding element (DUE) and initiates replication bacterial chromosome. Helicobacter pylori was previously identified as a region localized upstream dnaA containing cluster boxes bound by with high affinity. However, no sequence has been detected. Comprehensive in silico analysis presented this work allowed us identify an additional (oriC2), separated from original one (oriC1) gene. specifically binds both regions, but...
Campylobacter jejuni is the leading bacterial food-borne pathogen in Europe. Despite accepted limits of cultural detection fastidious bacterium, "gold standard" food microbiology still determination colony-forming units (CFU). As an alternative, a live/dead differentiating qPCR has been established, using propidium monoazide (PMA) as DNA-intercalating crosslink agent for inactivating DNA from dead, membrane-compromised cells. The PMA treatment was combined with addition internal sample...
Bacterial chromosome replication is mediated by single initiator protein, DnaA, that interacts specifically with multiple DnaA boxes located within the origin (oriC). We compared architecture of DnaA-origin complexes evolutionarily distantly related eubacteria: two Gram-negative organisms, Escherichia coli and Helicobacter pylori, Gram-positive Mycobacterium tuberculosis Streptomyces coelicolor. Their origins vary in size (from approx. 200 to 1000 bp) number 5 19). The results indicate that:...
Summary Replication of the bacterial chromosome is initiated by binding DnaA protein to a unique DNA region, called oriC . Many regulatory factors in numerous species act controlling ability bind and unwind DNA, but Helicobacter pylori genome does not contain homologues any these factors. Here, we describe HobA, novel essential for initiation H. replication, which conserved among, to, epsilon proteobacteria. We demonstrate that HobA interacts specifically via with –DnaA complex. postulate...
As a neutrophilic bacterium, Helicobacter pylori is growth deficient under extreme acidic conditions. The gastric pathogen equipped with an acid survival kit, regulating urease activity by pH-gated urea channel, opening below pH 6.5. After overcoming stress, the bacterium's multiplication site situated at mucosa near neutral pH. exhibits exceptional genetic variability, mainly due to its capability of natural transformation, termed competence. Using single cell analysis, we show here that...
Abstract Background Serine protease HtrA exhibits both proteolytic and chaperone activities, which are involved in cellular protein quality control. Moreover, is an important virulence factor many pathogens including Helicobacter pylori , for the crucial stage of infection cleavage E-cadherin other cell-to-cell junction proteins. Methods The vitro study H. (HtrA Hp ) activity was carried out using light scattering assays investigation lysozyme aggregates. We produced ∆ htrA deletion point...
Nickel homeostasis in<italic>Helicobacter pylori</italic>and potential histidine-rich binding sites from various bacterial and fungal pathogens are discussed.
The replication of a bacterial chromosome is initiated by the DnaA protein, which binds to specific chromosomal region oriC and unwinds duplex DNA within DNA-unwinding element (DUE). initiation tightly regulated many factors, control either or activity ensure that duplicated only when conditions favor survival daughter cells. factors controlling often belong protein families two-component systems. Here, we found Helicobacter pylori controlled HP1021, member atypical response regulator...
Abstract Helicobacter pylori plays an essential role in the pathogenesis of gastritis, peptic ulcer disease, and gastric cancer. The serine protease HtrA, important secreted virulence factor, disrupts epithelium, which enables H . to transmigrate across epithelium inject oncogenic CagA protein into host cells. function periplasmic HtrA for cell is unknown, mainly due unavailability htrA mutants. In fact, has been described as gene this bacterium. We have screened 100 worldwide isolates show...
The gastric human pathogen Helicobacter pylori has developed mechanisms to combat stress factors, including reactive oxygen species (ROS). Here, we present a comprehensive study on the redox switch protein HP1021 regulon combining transcriptomic, proteomic and DNA-protein interactions analyses. Our results indicate that modulates H. pylori's response oxidative stress. controls transcription of 497 genes, 407 genes related 79 proteins are differently expressed in deletion mutant. typical ROS...
Two novel coordination polymers, [Bi2(2,3pydc)2(2,3pydcH)2(H2O)]n (1) and {(Et3NH)2[Bi(2,3pydc)(2,3pydcH)Cl2]}n (2) were prepared using as a prolinker pyridine-2,3-dicarboxylic acid (2,3pydcH2). The obtained complexes fully characterized by elemental analysis, TG/DTG, FT-IR, solid-state photoluminescence, DFT calculations single-crystal X-ray diffraction. crystallized in the triclinic P-1 space group comprise dimeric units with two crystallographically different Bi(III) centers (polyhedra:...
Abstract Helicobacter pylori is a gram-negative, microaerophilic, pathogenic bacterium and widespread colonizer of humans. H. has developed mechanisms that enable it to overcome the harsh environment human stomach, including reactive oxygen species (ROS). Interestingly, up now no typical regulator dedicated oxidative-stress response been discovered. In this work, we reveal inhibitor replication initiation HP1021 functions as redox switch protein in plays an important role oxidative stress...
ABSTRACT In Streptomyces coelicolor , replication is initiated by the DnaA protein in centrally located oriC region and proceeds bidirectionally until forks reach ends of linear chromosome. We identified three clusters boxes (H69, H24, D78) which are a relatively short segment chromosome centered on region. Of analyzed, D78 exhibited highest affinity for protein; cluster was about eightfold higher than . The high-affinity appear to be involved control replication. Deletion resulted more...
Bdellovibrio bacteriovorus is a small Gram-negative predatory bacterium that attacks other bacteria, including many animal, human, and plant pathogens. This exhibits peculiar biphasic life cycle during which two different types of cells are produced: non-replicating highly motile (the free-living phase) replicating intracellular-growth phase). The process chromosomal replication in B. must therefore be temporally spatially regulated to ensure it coordinated with cell differentiation...
In bacteria, chromosome replication is initiated by the interaction of initiator protein DnaA with a defined region at which DNA starts (oriC). While proteins share significant homology regardless phylogeny, oriC regions exhibit more variable structures. The general architecture oriCs universal, i.e. they are composed cluster binding sites, DNA-unwinding element, and sequences that bind regulatory proteins. However, detailed structures shared related species while being significantly...
Campylobacter jejuni is the leading bacterial cause of foodborne infections worldwide. However, our understanding its cell cycle poor. We identified probable C. origin replication (oriC) - a key element for initiation chromosome replication, which also important structure, maintenance and dynamics. The herein characterized oriC monopartite contains (i) DnaA box cluster, (ii) DnaA-dependent DNA unwinding (DUE) (iii) binding sites regulatory proteins. cluster five boxes DUE were found in...