A5052817365- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Helicobacter pylori-related gastroenterology studies
- Listeria monocytogenes in Food Safety
- Genomics and Phylogenetic Studies
- Veterinary medicine and infectious diseases
- Bacterial Genetics and Biotechnology
- Food Safety and Hygiene
- Escherichia coli research studies
- Galectins and Cancer Biology
- Animal Nutrition and Physiology
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
- Probiotics and Fermented Foods
- Molecular Biology Techniques and Applications
- Microbial Applications in Construction Materials
- Microbial Metabolites in Food Biotechnology
- Clostridium difficile and Clostridium perfringens research
- Biochemical and Molecular Research
- Protein Hydrolysis and Bioactive Peptides
- RNA and protein synthesis mechanisms
- Gut microbiota and health
- Pancreatitis Pathology and Treatment
- Identification and Quantification in Food
Federal Institute for Risk Assessment
2016-2025
Institut Pasteur
2005-2010
University of Münster
2007-2009
Osnabrück University
2000-2002
University of Groningen
1998
WGS-based antimicrobial susceptibility testing (AST) is as reliable phenotypic AST for several antimicrobial/bacterial species combinations. However, routine use of hindered by the need bioinformatics skills and knowledge resistance (AMR) determinants to operate vast majority tools developed date. By leveraging on ResFinder PointFinder, two freely accessible that can also assist users without skills, we aimed at increasing their speed providing an easily interpretable antibiogram output.The...
Campylobacter infection is the most commonly notified bacterial enteritis in Germany. We performed a large combined case-control and source attribution study (Nov 2011-Feb 2014) to identify risk factors for sporadic intestinal infections determine relative importance of various animal sources human conducted multivariable logistic regression analysis factors. Source was using asymmetric island model based on MLST data animal/food isolates. As we considered chicken, pig, pet dog or cat,...
The presence of bacteria carrying antimicrobial resistance (AMR) genes in wildlife is an indicator that resistant human or livestock origin are widespread the environment. In addition, it could represent additional challenge for health, since wild animals act as efficient AMR reservoirs and epidemiological links between human, natural environments. aim this study was to investigate occurrence antibiotic patterns several bacterial species certain Germany, including boars (Sus scrofa), roe...
The Gram-negative gastric pathogen Helicobacter pylori depends on natural transformation for genomic plasticity, which leads to host adaptation and spread of resistances. Here, we show that H. takes up covalently labeled fluorescent DNA preferentially at the cell poles uptake is dependent type IV secretion system ComB. By titration external pH detection accessibility fluorophor by protons, localized imported in periplasm. Single molecule analysis revealed outer membrane transport occurred a...
In the human gastric bacterium Helicobacter pylori, two metalloenzymes, hydrogenase and urease, are essential for in vivo colonization, latter being a major virulence factor. The UreA UreB structural subunits of urease UreG, one accessory proteins Ni(2+) incorporation into apourease, were taken as baits tandem affinity purification. method allows purification protein complexes under native conditions physiological expression levels bait protein. Furthermore technology was combined with...
Bacteriophage treatment is a promising tool to reduce Campylobacter in chickens. Several studies have been published where group II or III phages were successfully applied. However, these two groups of are different regarding their host ranges and cell receptors. Therefore, concerted activity might enhance the efficacy decrease number resistant bacteria.In this study we compared lytic properties some analysed suitability various for reduction C. jejuni broiler We show that exhibit kinetics...
Abstract Background Campylobacter spp. is the most frequent cause of bacterial food-borne gastroenteritis and a high priority antibiotic resistant bacterium according to World Health Organization (WHO). European monitoring thermotolerant does not reflect global burden resistances already circulating within population worldwide. Methods We systematically compared whole genome sequencing with comprehensive phenotypic antimicrobial susceptibility, analyzing 494 poultry isolates from Vietnam...
ABSTRACT Bacterial plasmids play a major role in the spread of antibiotic resistance genes. However, their characterization via DNA sequencing suffers from low abundance plasmid those samples. Although sample preparation methods can enrich proportion before sequencing, these are expensive and laborious, they might introduce bias by enriching only for specific sequences. Nanopore adaptive sampling could overcome issues rejecting uninteresting molecules during process. In this study, we assess...
Carbon dioxide occupies a central position in the physiology of Helicobacter pylori owing to its capnophilic nature, large amounts carbon produced by urease-mediated urea hydrolysis, and constant bicarbonate supply stomach. Carbonic anhydrases (CA) catalyze interconversion are involved functions such as CO(2) transport or trapping pH homeostasis. H. encodes periplasmic alpha-CA (alpha-CA-HP) cytoplasmic beta-CA (beta-CA-HP). Single CA inactivation double were obtained for five genetic...
The unsuitability of the “CFU” parameter and usefulness cultivation-independent quantification Campylobacter on chicken products, reflecting actual risk for infection, is increasingly becoming obvious. Recently, real-time PCR methods in combination with use DNA intercalators, which block amplification from dead bacteria, have seen wide application. However, much confusion exists correct interpretation such assays. confronted by oxidative cold stress outside intestine. Hence, damage caused...
Campylobacter jejuni infections are progressively increasing worldwide. Probiotic treatment might open novel therapeutic or even prophylactic approaches to combat campylobacteriosis. In the present study secondary abiotic mice were generated by broad-spectrum antibiotic and perorally reassociated with a commensal murine Lactobacillus johnsonii strain either 14 days before (i.e. regimen) 7 after peroral C. 81-176 infection. Following reassociation both L. able stably colonize intestinal...
Abstract Conventional laboratory mice are protected from oral Campylobacter jejuni infection due to colonization resistance (CR) mediated by their host-specific gut microbiota. Here, we used differential effects of distinct antibiotics on microbiota composition identify microbial groups associated with CR against C. . Therefore, specific pathogen-free (SPF) were subjected ampicillin plus sulbactam (A/S), ciprofloxacin (CIP), or vancomycin (VAN) via the drinking water for 28 days left...
ABSTRACT The only enzyme of the citric acid cycle for which no open reading frame (ORF) was found in Helicobacter pylori genome is NAD-dependent malate dehydrogenase. Here, it shown that this organism oxidation to oxaloacetate catalyzed by a malate:quinone oxidoreductase (MQO). This flavin adenine dinucleotide-dependent membrane-associated donates electrons quinones electron transfer chain. Similar succinate dehydrogenase, part both chain and cycle. MQO activity demonstrated isolated...
Campylobacter species are the most prevalent bacterial pathogen causing acute enteritis worldwide. In contrast to jejuni, about 5 % of coli strains exhibit susceptibility restriction endonuclease digestion by DpnI cutting specifically 5’-GmATC-3’ motifs. This indicates significant differences in DNA methylation between both microbial species. The goal study was analyze methylome a C. strain susceptible digestion, identify its motifs and modification systems (RM-systems), compare them related...
Campylobacter jejuni (C. jejuni) is the most common cause of foodborne gastroenteritis worldwide. The bacteria induce diarrhea and inflammation by invading intestinal epithelium. Curcumin a natural polyphenol from turmeric rhizome Curcuma longa, medical plant, commonly used in curry powder. aim this study was investigation protective effects curcumin against immune-induced epithelial barrier dysfunction C. infection. indirect jejuni-induced defects its protection were analyzed co-cultures...
Raw milk is a frequent vehicle for transmission of thermophilic Campylobacter, leading to reported outbreaks. Milk challenging food matrix pathogen detection, due its high protein and lipid content. Limited detection Campylobacter CFU in raw might underestimate the pathogen’s infectious potential. We optimized viability qPCR application with milk. The procedure was robust against variations lots different strains. Various DNA-intercalating dyes were evaluated their ability reduce PCR signal...
The zoonotic pathogen Campylobacter jejuni is among the leading causes of foodborne diseases worldwide. While C. colonises many wild animals and livestock, persistence mechanisms enabling bacterium to adapt host species' guts are not fully understood. In order identify putative determinants influencing preferences distinct lineages, bootstrapping based on stratified random sampling combined with a k-mer-based genome-wide association was conducted 490 genomes from diverse origins in Germany...
ABSTRACT In the presence of urea neutrophilic human pathogen Helicobacter pylori survives for several hours at pH 1 with concomitant cytoplasmic homeostasis. To study this effect in detail, transmembrane proton motive force and urease activity H. were determined various values. absence urea, organism maintained a close-to-neutral cytoplasm an internally negative membrane potential external values greater than 4 to 5. accomplished homeostasis down 1.2. At pH, was 4.9 slightly inside. The...
In addition to their classical roles as carbon or nitrogen sources, amino acids can be used for bacterial virulence, colonization, stress resistance. We found that original deamidase-transport systems impact colonization by Helicobacter pylori, a human pathogen associated with gastric pathologies, including adenocarcinoma. demonstrated l-asparaginase (Hp-AnsB) and gamma-glutamyltranspeptidase (Hp-gammaGT) are highly active periplasmic deamidases in H. producing ammonia aspartate glutamate...
Campylobacter is the major bacterial agent of human gastroenteritis worldwide and represents a crucial global public health burden. Species differentiation C. jejuni coli phylogenetic analysis challenged by inter-species horizontal gene transfer. Routine real-time PCR on more than 4000 field strains identified isolates with ambiguous results for species differentiation, in particular, from isolation source eggs. K-mer whole genome sequencing data indicated presence hybrid huge amounts...