A5081552351- T-cell and B-cell Immunology
- RNA Research and Splicing
- Chemical Reactions and Isotopes
- RNA modifications and cancer
- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Monoclonal and Polyclonal Antibodies Research
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- Adipose Tissue and Metabolism
- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Single-cell and spatial transcriptomics
- Neurogenesis and neuroplasticity mechanisms
- Environmental Toxicology and Ecotoxicology
- RNA regulation and disease
- Circular RNAs in diseases
- Liver physiology and pathology
- Congenital heart defects research
- Systemic Lupus Erythematosus Research
- Cancer Immunotherapy and Biomarkers
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
University of Göttingen
2016-2025
Universitätsmedizin Göttingen
2016-2025
University Medical Center
2024
Charité - Universitätsmedizin Berlin
2024
University Hospital Cologne
2024
University of Helsinki
2024
University Hospital Leipzig
2024
Janssen (Netherlands)
2023
China Agricultural University
2023
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2022
Generation of homogeneous populations subtype-specific cardiomyocytes (CMs) derived from human induced pluripotent stem cells (iPSCs) and their comprehensive phenotyping is crucial for a better understanding the subtype-related disease mechanisms as tools development chamber-specific drugs. The goals this study were to apply simple efficient method differentiation iPSCs into defined functional CM subtypes in feeder-free conditions obtain molecular, cell biological, properties atrial...
Investigating the effect of ferroptosis in tumour microenvironment to identify combinatory therapy for liver cancer treatment.Glutathione peroxidase 4 (GPx4), which is considered master regulator ferroptosis, was genetically altered murine models hepatocellular carcinoma (HCC) and colorectal (CRC) analyse on cells immune microenvironment. The findings served as foundation identification additional targets combine with ferroptotic inducer treatment HCC metastasis.Surprisingly,...
There is ample evidence that physical activity exerts positive effects on a variety of brain functions by facilitating neuroprotective processes and influencing neuroplasticity. Accordingly, numerous studies have shown continuous exercise can successfully diminish or prevent the pathology neurodegenerative diseases such as Alzheimer's disease in transgenic mouse models. However, long-term effect health aging WT mice has not been studied detail yet. Here, we show prolonged cognitive...
Abstract Aims After a myocardial infarction, the adult human heart lacks sufficient regenerative capacity to restore lost tissue, leading failure progression. Finding novel ways reprogram cardiomyocytes into state is major therapeutic goal. The epicardium, outermost layer of heart, contributes cardiovascular cell types forming and source trophic signals promote muscle growth during embryonic development. epicardium also essential for regeneration in zebrafish neonatal mice can be reactivated...
Abstract Background Human pluripotent stem cell‐derived muscle models show great potential for translational research. Here, we describe developmentally inspired methods the derivation of skeletal cells and their utility in tissue engineering with aim to model regeneration dystrophy vitro. Methods Key steps include directed differentiation human embryonic progenitors followed by primary secondary foetal myogenesis into three‐dimensional muscle. To simulate Duchenne muscular (DMD), a...
The peripheral nervous system harbors a remarkable potential to regenerate after acute nerve trauma. Full functional recovery, however, is rare and critically depends on Schwann cells that orchestrate breakdown resynthesis of myelin and, at the same time, support axonal regrowth. How meet high metabolic demand required for repair remains poorly understood. We here report injury induces adipocyte glial signaling identify adipokine leptin as an upstream regulator adaptation in regeneration....
Regulatory B cells (Bregs) are immunosuppressive that modulate immune responses through multiple mechanisms. The signals required for the differentiation and activation of these remain still poorly understood. We have already shown overexpression A PRoliferation-Inducing Ligand (APRIL) reduces incidence severity collagen-induced arthritis (CIA) in mice. Furthermore, we described APRIL, but not BAFF, promoted IL-10 production regulatory functions human cells. Therefore, hypothesized may be...
Abstract Mutations and activation of the PI3K signaling pathway in breast cancer cells have been linked to brain metastases. However, here we describe that some metastases samples protein expression components is restricted metastatic microenvironment. In contrast therapeutic effects inhibition on cells, reaction microenvironment less understood. Therefore aimed quantify activity metastasis investigate central nervous system (CNS) First, systematically metastases, performed a prospective...
Background: Noonan syndrome (NS) is a multisystemic developmental disorder characterized by common, clinically variable symptoms, such as typical facial dysmorphisms, short stature, delay, intellectual disability well cardiac hypertrophy. The underlying mechanism gain-of-function of the RAS–mitogen-activated protein kinase signaling pathway. However, our understanding pathophysiological alterations and mechanisms, especially associated cardiomyopathy, remains limited effective therapeutic...
BackgroundEffector functions of IgG Abs are regulated by their Fc N-glycosylation pattern. glycans that lack galactose and terminal sialic acid residues correlate with the severity inflammatory (auto)immune disorders have also been linked to protection against viral infection discussed in context vaccine-induced protection. In contrast, sialylated shown immunosuppressive effects.ObjectiveWe sought investigate glycosylation programming during germinal center (GC) reaction following...
Hidradenitis suppurativa (HS) is a chronic inflammatory disease, characterized by painful, purulent and destructive skin alterations in intertriginous areas.We investigated the expression role HS of granulocyte colony-stimulating factor (G-CSF), regulator neutrophil biology, as clinical signs neutrophilic granulocyte-driven inflammation are distinctive disease.Skin blood samples obtained from different cohorts patients with control individuals were assessed RNA sequencing, quantitative...
Murine hepatic NK cells exhibit adaptive features, with liver-specific adhesion molecules CXCR6 and CD49a acting as surface markers.We investigated human liver-resident CXCR6+ CD49a+ using RNA sequencing, flow cytometry, functional analysis. We further assessed the role of cytokines in generating these phenotypes from peripheral blood.Hepatic could be induced produce high quantities IFNγ TNFα, contrast to cells. sequencing confirmed a tolerant immature phenotype reduced expression markers...
Genome editing by CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 is evolving rapidly. Recently, second-generation CRISPR/Cas9 activation systems based on nuclease inactive dead (d)Cas9 fused to transcriptional transactivation domains were developed for directing specific guide (g)RNAs regulatory regions of any gene interest, enhance transcription. The application dCas9 activate cardiomyocyte transcription in targeted genomic loci vivo has not been demonstrated so far.
For specific primary modes of action (MoA) in environmental non-target organisms, EU legislation restricts the usage active substances pesticides or biocides. Corresponding regulatory hazard assessments are costly, time consuming and require large numbers non-human animal studies. Currently, predictive toxicology development compounds relies on their chemical structure provides little insights into toxicity mechanisms that precede adverse effects. Using zebrafish embryo model, we...
Abstract When mRNAs have been transcribed and processed in the nucleus, they are exported to cytoplasm for translation. This export is mediated by receptor heterodimer Mex67–Mtr2 yeast Saccharomyces cerevisiae (TAP–p15 humans) 1,2 . Interestingly, many long non-coding RNAs (lncRNAs) also leave nucleus but it currently unclear why move 3 Here we show that antisense (asRNAs) accelerate mRNA annealing with their sense counterparts through helicase Dbp2. These double-stranded (dsRNAs) dominate...
The transcriptomic classification of primary colorectal cancer (CRC) into distinct consensus molecular subtypes (CMSs) is a well‐described strategy for patient stratification. However, the nature CRC metastases remains poorly investigated. To this end, study aimed to identify and compare organotropic CMS frequencies in liver brain metastases. Compared reported CRC, from patients were CMS4‐enriched CMS3‐depleted, whereas mainly clustered as CMS3 rarely CMS4. Regarding overall survival rates,...