A5011726101- Liver physiology and pathology
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Cancer Research and Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Colorectal Cancer Treatments and Studies
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Advanced biosensing and bioanalysis techniques
- Gene expression and cancer classification
- Ferroptosis and cancer prognosis
- Growth Hormone and Insulin-like Growth Factors
- Lung Cancer Treatments and Mutations
- DNA Repair Mechanisms
- Medical Imaging Techniques and Applications
- Cancer, Stress, Anesthesia, and Immune Response
- Plant Pathogens and Fungal Diseases
- Radiomics and Machine Learning in Medical Imaging
- Mechanisms of cancer metastasis
- RNA and protein synthesis mechanisms
- Hedgehog Signaling Pathway Studies
- Cardiac tumors and thrombi
- Pituitary Gland Disorders and Treatments
- Bioinformatics and Genomic Networks
- Cancer-related molecular mechanisms research
Universitätsmedizin Göttingen
2016-2025
University of Göttingen
2017-2025
BG University Hospital Bergmannsheil Bochum
2024
Oregon National Primate Research Center
1992
Abstract Mutations and activation of the PI3K signaling pathway in breast cancer cells have been linked to brain metastases. However, here we describe that some metastases samples protein expression components is restricted metastatic microenvironment. In contrast therapeutic effects inhibition on cells, reaction microenvironment less understood. Therefore aimed quantify activity metastasis investigate central nervous system (CNS) First, systematically metastases, performed a prospective...
Breast cancer is a heterogeneous disease and has been classified into five molecular subtypes based on gene expression profiles. Signaling processes linked to different breast clinical outcomes are still poorly understood. Aberrant regulation of Wnt signaling implicated in progression. In particular Ror1/2 receptors several other members the non-canonical pathway were associated with aggressive behavior. However, signals mediated via multiple complex pathways it clinically important...
The transcriptomic classification of primary colorectal cancer (CRC) into distinct consensus molecular subtypes (CMSs) is a well‐described strategy for patient stratification. However, the nature CRC metastases remains poorly investigated. To this end, study aimed to identify and compare organotropic CMS frequencies in liver brain metastases. Compared reported CRC, from patients were CMS4‐enriched CMS3‐depleted, whereas mainly clustered as CMS3 rarely CMS4. Regarding overall survival rates,...
As a major component of intracellular trafficking, the coat protein complex II (COPII) is indispensable for cellular function during embryonic development and throughout life. The four SEC24 proteins (A-D) are essential COPII components involved in cargo selection packaging. A human disorder corresponding to alterations currently only known SEC24D. Here, we report that biallelic loss SEC24C leads syndrome characterized by primary microcephaly, brain anomalies, epilepsy, hearing loss, liver...
Background Pipeline comparisons for gene expression data are highly valuable applied real analyses, as they enable the selection of suitable analysis strategies dataset at hand. Such pipelines RNA-Seq should include mapping reads, counting and differential or preprocessing, normalization in case microarray analysis, order to give a global insight into pipeline performances. Methods Four commonly used (STAR/HTSeq-Count/edgeR, STAR/RSEM/edgeR, Sailfish/edgeR, TopHat2/Cufflinks/CuffDiff)) were...
Recent evidence has implicated the transforming growth factor-alpha (TGF alpha)/epidermal factor receptor (EGFR) system in mechanism by which hypothalamic lesions accelerate female sexual development. Since acquisition and maintenance of reproductive functions depend on secretory activity LHRH neurons, present studies were undertaken to characterize some cellular molecular events that underlie lesion-induced activation neuronal network. Bilateral electrolytic posterior portion preoptic...
Transcription factors (TFs) are gene regulatory proteins that essential for an effective regulation of the transcriptional machinery. Today, it is known their expression plays important role in several types cancer. Computational identification key players specific cancer cell lines still open challenge research. In this study, we present a systematic approach which combines colorectal (CRC) lines, namely 1638N-T1 and CMT-93, well-established computational methods order to compare these on...
Seventy percent of patients with colorectal cancer develop liver metastases (CRLM), which are a decisive factor in progression. Therapy outcome is largely influenced by tumor heterogeneity, but the intra- and inter-patient heterogeneity CRLM has been poorly studied. In particular, contribution WNT EGFR pathways, both frequently deregulated cancer, not yet addressed this context. To end, we comprehensively characterized normal tissue eight from two standardized histopathological, molecular,...
Background: Sarculator and Memorial Sloan Kettering Cancer Center (MSKCC) nomograms are freely available risk prediction scores for surgically treated patients with primary sarcomas. Due to the rarity of angiosarcomas, these have only been tested on small cohorts angiosarcoma patients. In neither original patient cohort upon which is based nor in subsequent studies was a distinction made between secondary as app intended be applied Therefore, objective our investigation assess whether...
Colorectal cancer (CRC) is the second leading cause of cancer-related death in men and women. Systemic disease with metastatic spread to distant sites such as liver reduces survival rate considerably. The aim this study was investigate changes gene expression that occur on invasion expansion CRC cells when forming metastases liver. livers syngeneic C57BL/6NCrl mice were inoculated 1 million (CMT-93) via portal vein, stable formation within 4 weeks. RNA sequencing performed Illumina platform...
Ubiquitous overactivation of Hedgehog signaling in adult pituitaries results increased expression proopiomelanocortin ( Pomc ), growth hormone Gh ) and prolactin Prl elevated adrenocorticotropic (Acth) production proliferation Sox2 + cells. Moreover, ACTH, GH PRL-expressing human pituitary adenomas strongly express the target GLI1. Accordingly, seems to play an important role pathology probably also homeostasis hypophysis. However, specific Hedgehog-responsive cell type has not yet been...
Somatic single nucleotide variants (SNVs) are genomic events with increasing implications in cancer treatment. The clinical standard for SNVs detection is whole genome/exome sequencing (WGS/WES) matched tumor-normal samples. Yet, this a very costly approach both economically and biologically often only tumor samples sequenced. On the other hand, RNA (RNA-Seq) most popular technology to study gene expression, has also potential cost-effective identification of as an alternative tumor-only...
Bloom syndrome (BS) is an autosomal recessive disease clinically characterized by primary microcephaly, growth deficiency, immunodeficiency and predisposition to cancer. It mainly caused biallelic loss-of-function mutations in the BLM gene, which encodes helicase, acting DNA replication repair processes. Here, we describe gene expression profiles of three BS fibroblast cell lines harboring causative, truncating obtained single-cell (sc) transcriptome analysis. We compared scRNA transcription...
The description of the myotrophic activity assay in rat using levator ani muscle (Eisenberg & Gordon, 1950) has been followed by many modifications. These changes include reduction time (Hershberger, Shipley Meyer, 1953; Metcalf Broich, 1961; Potts, Arnold Beyler, 1960; Sala Baldratti, 1963), a suggested change nomenclature (Hayes, 1965) and varying statistical interpretations (Ahrén, Arvill Hjalmarson, 1962; Ercoli, Bruni, Falconi, Gardi Meli, Hershberger et al. 1953). Although progress...
Abstract Bloom syndrome (BS) is an autosomal recessive disease clinically characterized by primary microcephaly, growth deficiency, immunodeficiency, and predisposition to cancer. It mainly caused biallelic loss-of-function mutations in the BLM gene, which encodes helicase, acting DNA replication repair processes. Here, we describe gene expression profiles of three BS fibroblast cell lines harboring causative, truncating obtained single-cell (sc) transcriptome analysis. We compared scRNA...