Peritoneal dialysis (PD) is limited by glucose-mediated peritoneal membrane (PM) fibrosis, angiogenesis, and ultrafiltration failure. Influencing PM integrity pharmacologically targeting sodium-dependent glucose transporter (SGLT)-mediated uptake has not been studied. In this study, wildtype C57Bl/6N mice were treated with high-glucose dialysate via an intraperitoneal catheter, or without addition of selective SGLT2 inhibitor dapagliflozin. structural changes, capacity, equilibration testing...

Septic arthritis of the wrist is a rare but severe condition requiring urgent diagnosis and treatment to prevent joint destruction functional impairment. The objective this study was investigate prognostic parameters long-term outcomes. This retrospective prospective cohort included 44 patients treated for septic between 2008 2024. All underwent surgical arthrotomy due concomitant soft tissue involvement, with median follow-up 29 months. Clinical outcomes were assessed through total active...

Abstract Introduction Predicting burn mortality remains a critical aspect of care, guiding clinical decision making. Recent studies have highlighted the limitations traditional scores such as Baux or ABSI, and modified versions existing been introduced over time. Advances in care - including wound resuscitation protocols practices underscore need for updated prediction models that reflect modern treatment. In particular, potential new technologies, particularly machine learning, offers...

Background: Non-otherwise specified (NOS) sarcomas, a diverse and diagnostically challenging group of mesenchymal malignancies, pose significant clinical dilemmas due to their variable trajectories therapeutic responses. This study utilizes advanced machine learning techniques, namely classification regression trees Shapley additive explanation (SHAP) values, identify predictors survival, metastatic progression, recurrence within well-defined patient cohort, aiming improve risk...

The optimal therapy for deep burn wounds is based on the early debridement of necrotic tissue followed by wound coverage to avoid a systemic inflammatory response and optimize scar-free healing. outcomes are affected available resources underlying patient factors, which represent challenges in care suboptimal outcomes. In this study, we aimed determine burn-wound management using enzymatic (NexoBrid™, MediWound Germany GmbH, Rüsselsheim, Germany) intact fish skin (Kerecis

Abstract Bone infections are a frequent cause for large bony defects with reduced healing capacity. In previous findings, we could already show diminished capacity after bone infections, despite the absence of causing agent, Staphylococcus aureus. Moreover, these showed osteoblastogenesis and increased osteoclastogenesis, meaning elevated resorption ongoing an B-cell activity. To overcome negative effects this postinfectious inflammatory state, tried to use regenerative mesenchymal stem...

Secondary lymphedema is a very common clinical issue with millions of patients suffering from pain, recurrent skin infections, and the constant need for decongestive therapy. Well-established as consequence oncologic procedures, secondary also well-known phenomenon after trauma. However, precise epidemiological data progress upon severe extremity injuries are still missing. In present work, we analyzed patient cohort 94 individuals who suffered open fractures lower soft tissue injury, 2nd...

Abstract Introduction Many studies have started to search for the perfect aesthetic breast in order create a pars-pro-toto reconstruction, but especially surgery. To date, no representative study with anatomically accurate models was performed. Methods In an online based United-States-census-representative survey 1049 participants, questions regarding preferred were asked utilizing lifelike morphed 3D-generated female first time. Attributes such as pole ratio, areola size, direction and...

Acute ischemia reperfusion injury in skeletal muscle remains an important issue several fields of regenerative medicine. Thus, a valid model is essential to gain deeper insights into pathophysiological relations and evaluate possible treatment options. While the vascular anatomy mice regularly prevents sufficient vessel occlusion by invasive methods, there multitude existing models induce without surgical procedures. Since no consensus on which prefer, this study aims develop novel,...

Ischemia reperfusion (IR) injury remains an important topic in clinical medicine. While a multitude of prophylactic and therapeutic strategies have been proposed, recent studies illuminated protective effects myostatin inhibition. This study aims to elaborate on the intracellular pathways involved signaling explore key proteins that convey IR injury. We used CRISPR/Cas9 gene editing introduce (Mstn) deletion into C2C12 cell line. In subsequent experiments, we evaluated overall death,...

As a rule, undergraduate medical students experience everyday work in health care as spectators. They are not allowed to participate real-life interaction between professionals and patients. We report on an exception this rule.The aim of study was examine students' experiences developing their first professional-patient relationships the basis being responsible for patients.The involved 2 cohorts (2002 2003) students, amounting 503 students. had all worked hospitals and/or nursing homes 4...

<b><i>Background:</i></b> Burn injury leads to a hypercatabolic response and ultimately muscle wasting with drastic implications for recovery of bodily functions, patient’s quality life (QoL), long-term survival. Several treatment options target the body’s initial stress response, but pharmacological approaches specifically address protein metabolism have only been poorly investigated. <b><i>Objective:</i></b> The aim this study was assess role...

Abstract Background Delayed bone healing, especially in long bones poses one of the biggest problems orthopeadic and reconstructive surgery causes tremendous costs every year. There is a need for exploring order to find an adequate therapy. Earlier investigations human scaphoid non-union revealed elevated osteoclast activity, accompanied by upregulated levels TGF-beta RANKL. Interestingly, seemed be well vascularized. Methods In current study, we used murine femur-defect model study atrophic...

Background Excessive inflammation, hemolysis, and accumulation of labile heme play an essential role in the pathophysiology multi-organ dysfunction syndrome (MODS) sepsis. Alpha1-antitrypsin (AAT), acute phase protein with binding capacity, is one modulators host responses to inflammation. In this study, we evaluate putative protective effect AAT against MODS mortality a mouse model polymicrobial abdominal Methods Polymicrobial sepsis was induced C57BL/6N mice by cecal ligation puncture...

Background: Sarculator and Memorial Sloan Kettering Cancer Center (MSKCC) nomograms are freely available risk prediction scores for surgically treated patients with primary sarcomas. Due to the rarity of angiosarcomas, these have only been tested on small cohorts angiosarcoma patients. In neither original patient cohort upon which is based nor in subsequent studies was a distinction made between secondary as app intended be applied Therefore, objective our investigation assess whether...

Abstract Impaired bone homeostasis caused by osteomyelitis provokes serious variations in the remodeling process, thereby involving multiple inflammatory cytokines to activate healing. We have previously established a mouse model for post-traumatic and studied regeneration after sufficient debridement. Moreover, we could further characterize postinfectious state of bony defects debridement with elevated osteoclasts decreased formation despite absence bacteria. In this study, investigated...

Abstract Ischemia reperfusion (IR) injury plays a pivotal role in many diseases and leads to collateral damage during surgical interventions. While most studies focus on alleviating its severity the context of brain, liver, kidney, cardiac tissue, research as regards skeletal muscle has not been conducted same extent. In past, myostatin (MSTN), primarily known for supressing growth, implicated inflammatory circuits, provided promising results IR mitigation by inhibiting MSTN cell surface...

Tumors, traumata, burn injuries or surgeries can lead to critical-sized bony defects which need be reconstructed. Mesenchymal stem cells (MSCs) have the ability differentiate into multiple cell lineages and thus present a promising alternative for use in tissue engineering reconstruction. However, there is an ongoing debate whether all MSCs are equivalent their differentiation proliferation ability. The goal of this study was assess osteogenic adipogenic characteristic changes...

ABSTRACT Peritoneal dialysis (PD) is limited by glucose-mediated peritoneal membrane (PM) fibrosis, angiogenesis and ultrafiltration failure. Influencing PM integrity pharmacologically targeting sodium-dependent glucose transporter (SGLT)-mediated uptake has not been studied. In this study wildtype C57Bl/6N mice were treated with high-glucose dialysate via an intraperitoneal catheter, or without addition of selective SGLT2 inhibitor dapagliflozin. structural changes, capacity PET status for...

The female breast is a symbol of femininity and plays key role in the body image. However, factors influencing preferences for different shapes sizes are still not elucidated. In particular, emerging social media perception has been analyzed yet. A representative cohort 1,049 adults completed web-based questionnaire containing hyperrealistic 3D models United States. machine-learning algorithm (Classification Regression Tree [CART]) was implemented to identify most influential factors. study...

Zusammenfassung Hintergrund Die Behandlung Schwerbrandverletzter ist anspruchsvoll und erfordert spezialisierte Zentren, die eine adäquate Therapie über einen Zeitraum von bis zu mehreren Monaten gewährleisten können. Etablierung digitalen Managementsystemen auf Intensivstationen markiert immensen Fortschritt in der modernen Gesundheitsversorgung. Einführung eines solchen Systems einer spezialisierten Intensivstation für Schwerbrandverletzte bietet diverse Möglichkeiten zur Optimierung, aber...

Abstract Introduction Classic heat stroke is a severe trauma which can lead to multi-organ dysfunctions and associated with high mortality. Case presentation In this case report we present 73-year-old patient classic stroke. His initial core body temperature was over 42 °C he had GCS of 3. Due burn injuries the transferred specialized center. The developed different organ failures showed prolonged course on intensive care unit. Although demonstrated impaired systems, recovered completely...