A5061863999- Heme Oxygenase-1 and Carbon Monoxide
- Organ Transplantation Techniques and Outcomes
- Neonatal Health and Biochemistry
- Acute Kidney Injury Research
- Immune cells in cancer
- Renal Transplantation Outcomes and Treatments
- Hematopoietic Stem Cell Transplantation
- Immune Response and Inflammation
- Mitochondrial Function and Pathology
- Hemoglobin structure and function
- Transplantation: Methods and Outcomes
- Cytomegalovirus and herpesvirus research
- ATP Synthase and ATPases Research
- Thermal Regulation in Medicine
- Epigenetics and DNA Methylation
- Autophagy in Disease and Therapy
- Cardiac Arrest and Resuscitation
- Immune Cell Function and Interaction
- Neurological Complications and Syndromes
- Alcohol Consumption and Health Effects
- Mechanical Circulatory Support Devices
- Blood transfusion and management
- Cardiomyopathy and Myosin Studies
- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
Medizinische Hochschule Hannover
2015-2024
Stanford University
2021-2024
Palo Alto University
2023
RELX Group (United States)
2018
Justus-Liebig-Universität Gießen
2009-2017
Institute for Transfusion Medicine
2017
Giessen School of Theology
2014
Macrophages adopt different phenotypes in response to microenvironmental changes, which can be principally classified into inflammatory and anti-inflammatory states. Inflammatory activation of macrophages has been linked with metabolic reprogramming from oxidative phosphorylation aerobic glycolysis. In contrast mouse macrophages, little information is available on the link between metabolism inflammation human macrophages. current report it demonstrated that lipopolysaccharide...
Idiopathic pulmonary fibrosis (IPF) is a devastating disease, and its pathogenic mechanisms remain incompletely understood. Peroxisomes are known to be important in ROS proinflammatory lipid degradation, their deficiency induces liver fibrosis. However, altered peroxisome functions IPF pathogenesis have never been investigated. By comparing peroxisome-related protein gene expression lung tissue isolated fibroblasts between human control patients, we found that lungs exhibited significant...
Abstract Heme oxygenase (HO)-1 is the inducible isoform of first and rate-limiting enzyme heme degradation. The HO products carbon monoxide bilirubin not only provide antioxidant cytoprotection, but also have potent anti-inflammatory immunomodulatory functions. Although HO-1 has previously been shown to be induced by various stimuli via activation p38 MAPK signaling pathway, role this protein kinase for gene regulation largely unknown. In present study, it demonstrated that pharmacological...
Summary Despite optimal therapy, the morbidity and mortality of patients presenting with an acute myocardial infarction (MI) remain significant, initial mechanistic trigger “ischaemia/reperfusion (I/R) injury” remains greatly unexplained. Here we show that factors released from damaged cardiac tissue itself, in particular extracellular RNA (eRNA) tumour-necrosis-factor α (TNF-α), may dictate I/R injury. In experimental vivo mouse model as well isolated Langendorff-perfused rat heart,...
Abstract Spermatogenic cells express cell-specific molecules with the potential to be seen as “foreign” by immune system. Owing time difference between their appearance in puberty and editing of lymphocyte repertoire around birth, local adaptations system coined privilege are required confer protection from autoattack. Testicular macrophages (TM) play an important role maintaining testicular display reduced proinflammatory capacity compared other macrophages. However, molecular mechanism...
Heme oxygenase (HO)-1 is the inducible isoform of rate-limiting enzyme heme degradation and provides cytoprotection against oxidative stress by its products carbon monoxide biliverdin. More recently, HO-1 has also been shown to exert immunomodulatory functions via cell type-specific anti-inflammatory effects in myeloid/macrophage cells. In current study, it demonstrated that Bruton's tyrosine kinase (Btk), gene which mutated human immunodeficiency X-linked agammaglobulinemia, involved...
Abstract Peroxisomes are proposed to play an important role in the regulation of systemic inflammation; however, functional these organelles inflammatory responses myeloid immune cells is largely unknown. In this article, we demonstrate that nonclassical peroxisome proliferator 4-phenyl butyric acid efficient inducer peroxisomes various models murine macrophages, such as primary alveolar and peritoneal macrophages macrophage cell line RAW264.7, but not bone marrow–derived macrophages....
Activation of inflammation is tightly associated with metabolic reprogramming in macrophages. The iron-containing tetrapyrrole heme can induce pro-oxidant and pro-inflammatory effects murine macrophages, but has been polarization towards an anti-inflammatory phenotype human In the current study, we compared regulatory responses to prototypical Toll-like receptor (TLR)4 ligand lipopolysaccharide (LPS) mouse macrophages a particular focus on alterations cellular bioenergetics. bulk...
Heme oxygenase (HO)-1, a stress-inducible enzyme that converts heme into carbon monoxide (CO), iron and biliverdin, exerts important anti-inflammatory effects in activated macrophages. HO-1 expression is mainly governed by mutual interplay between the transcriptional factor NRF2 nuclear repressor BTB CNC homology 1 (BACH1), sensor protein. In current study we hypothesized alterations levels of intracellular labile macrophages stimulated lipopolysaccharide (LPS), prototypical pro-inflammatory...
BTB-and-CNC homologue 1 (BACH1), a heme-regulated transcription factor, mediates innate immune responses via its functional role in macrophages. BACH1 has recently been shown to modulate mitochondrial metabolism cancer cells. In the current study, we utilized proteomics approach and demonstrate that genetic deletion of mouse macrophages is associated with decreased levels various proteins, particularly complex I. Bioenergetic studies revealed alterations energy BACH1-/- shift towards...
Heme is a ubiquitous compound of human tissues, and it involved in cellular physiology metabolism. Once released from the cell, free heme oxidizes to ferric state (hemin). High levels hemin can cause oxidative stress inflammation if not neutralized immediately by specialized scavenger proteins. Human alpha1-antitrypsin (A1AT), an acute-phase glycoprotein important inhibitor neutrophil proteases, also hemin-binding protein. A short-term exposure freshly isolated blood neutrophils 4 µM results...
Background: Ischemia reperfusion injury (IRI) plays a major role in solid organ transplantion. The length of warm ischemia time is critical for the extent tissue damage renal IRI. In this experimental study we hypothesized that local release labile heme triggered by duration (15 versus 45 min IRI) and mediates complement activation, cytokine inflammation. Methods: To induce IRI, pedicle clamping was performed male C57BL/6 mice short min) or prolonged (45 periods. Two 24 h after levels kidney...
Recent publications have shown that mitochondrial dynamics can govern the quality and quantity of extracellular mitochondria subsequently impacting immune phenotypes. This study aims to determine if pathologic fission mediated by Drp1/Fis1 interaction impacts content macrophage function in sepsis-induced immunoparalysis.
Ischemia reperfusion injury (IRI) is linked with inflammation in kidney transplantation (ktx). The chemokine CXCL13, also known as B lymphocyte chemoattractant, mediates recruitment of cells within follicles lymphoid tissues and has recently been identified a biomarker for acute allograft rejection. goal this study was to explore whether IRI contributes the up-regulation CXCL13 levels ktx. It demonstrated that systemic were increased mouse models uni- bilateral renal IRI, which correlated...
Abstract Antibody-mediated rejection (AMR) is the major cause of allograft loss after solid organ transplantation. Circulating donor-specific antibodies against human leukocyte antigen (HLA), in particular HLA class II are critical for pathogenesis AMR via interactions with endothelial cells (ECs). To investigate effects antibody ligation to graft endothelium, a model HLA-DR antibody-dependent stimulation was utilized primary ECs. Antibody molecules interferon-γ-treated ECs caused necrotic...