A5025069419- Renal Transplantation Outcomes and Treatments
- Organ Transplantation Techniques and Outcomes
- Neurological Complications and Syndromes
- Acute Kidney Injury Research
- Complement system in diseases
- Chronic Kidney Disease and Diabetes
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- MRI in cancer diagnosis
- Cytomegalovirus and herpesvirus research
- Heme Oxygenase-1 and Carbon Monoxide
- Renal and Vascular Pathologies
- Viral Infections and Outbreaks Research
- COVID-19 epidemiological studies
- Immune responses and vaccinations
- Renal Diseases and Glomerulopathies
- Viral Infections and Vectors
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Renin-Angiotensin System Studies
- Advanced MRI Techniques and Applications
- Pregnancy and preeclampsia studies
- Venomous Animal Envenomation and Studies
- Artificial Intelligence in Healthcare
- Cardiac electrophysiology and arrhythmias
- Diabetes Treatment and Management
Medizinische Hochschule Hannover
2014-2020
Bayer (Germany)
2019
RELX Group (United States)
2018
Bernhard Nocht Institute for Tropical Medicine
2016
PHV Dialysezentrum
2015
University of Veterinary Medicine Hannover, Foundation
2012
University of Veterinary Medicine Vienna
2012
A number of previous studies have identified antigen-presenting cells (APCs) as key targets Ebola virus (EBOV), but the role APCs in human disease (EVD) is not known. We evaluated phenotype and kinetics monocytes, neutrophils, dendritic (DCs) peripheral blood patients for whom EVD was diagnosed by European Mobile Laboratory Guinea. Acute characterized reduced levels circulating nonclassical CD16+ monocytes with a poor activation profile. In survivors, were activated during recovery,...
Kidney transplantation (ktx) in mice is used to learn about rejection and develop new treatment strategies. Past studies have mainly been based on histological or molecular biological methods. Imaging techniques monitor allograft pathology rarely used.Here we investigated after isogenic allogenic ktx over time with functional MRI diffusion-weighted imaging (DWI) mapping of T2-relaxation (T2-mapping) assess graft inflammation edema formation. To characterize pathology, PAS-staining, counted...
Background: Ischemia reperfusion injury (IRI) plays a major role in solid organ transplantion. The length of warm ischemia time is critical for the extent tissue damage renal IRI. In this experimental study we hypothesized that local release labile heme triggered by duration (15 versus 45 min IRI) and mediates complement activation, cytokine inflammation. Methods: To induce IRI, pedicle clamping was performed male C57BL/6 mice short min) or prolonged (45 periods. Two 24 h after levels kidney...
Renal ischemia-reperfusion injury (IRI) is a severe complication of major surgery and risk factor for increased morbidity mortality. Here, we investigated mechanisms that might contribute to IRI-induced progression chronic kidney disease (CKD). Acute (AKI) was induced by unilateral IRI 35 min in CD1 C57BL/6 (B6) mice. Unilateral used overcome early morphology, NGAL upregulation, neutrophil infiltration as well peritubular capillary density were studied immunohistochemistry. The composition...
The presence of B-cell clusters in allogenic T cell-mediated rejection (TCMR) kidney allografts is linked to more severe disease entities. In this study we characterized infiltrates patients with TCMR and examined the role serum CXCL-13 these experimentally. levels were analyzed 73 allograft recipients at time biopsy. addition, four evaluated for CXCL13 during first week after transplantation. ELISA was done measure levels. For further mechanistic understanding, a translational transplant...
Purpose To examine the longitudinal changes of renal perfusion due to acute and chronic allograft rejection by using arterial spin labeling (ASL) MRI in translational mouse models isogenic allogenic kidney transplantation (ktx). Materials Methods Acute was induced ktx C57BL/6 (B6)‐kidney grafts BALB/c‐recipients with prolonged cold ischemia (CIT) 60 minutes ( n = 13). induce BALB/c‐kidneys were transplanted into B6‐recipients short CIT 30 22). Isogenic without 14 prolonged, 9 CIT) normal...
Abstract Background Voltage-gated sodium channels generate action potentials in excitable cells, but they have also been attributed noncanonical roles nonexcitable cells. We hypothesize that voltage-gated play a functional role during extravasation of neutrophils. Methods Expression was analyzed by polymerase chain reaction. Distribution Nav1.3 determined immunofluorescence and flow cytometry mouse models ischemic heart kidney injury. Adhesion, transmigration, chemotaxis neutrophils to...
Systemic exposure to high-dose corticosteroids effectively combats acute rejection after kidney transplantation, but at the cost of substantial side effects. In this study, a murine renal allograft model was used investigate whether liposomal-encapsulated prednisolone (LP) facilitates local enhance its therapeutic effect.Male BalbC recipients received allografts from male C57BL/6J donors. Recipients were injected daily with 5 mg/kg cyclosporine A and either 10 (P), or LP intravenously on day...
IL-17A contributes to acute kidney injury and fibrosis. Therefore, we asked whether deficiency or treatment with a blocking antibody impacts severe renal ischaemia reperfusion (IRI) the progression chronic disease (CKD).IL-17A-deficient wild-type (WT) mice underwent transient unilateral pedicle clamping for 45 min induce IRI subsequent Furthermore, neutralizing anti-IL-17A (mAb) was injected into WT before induction of intravenously. On days 1, 7 21, inflammation, fibrosis, leukocyte...
Acute kidney injury (AKI) frequently complicates major surgery and can be associated with hypertension progress to chronic disease, but reports on blood pressure normalization in AKI are conflicting. In the present study, we investigated effects of an angiotensin-converting enzyme inhibitor, enalapril, a soluble epoxide hydrolase 1-trifluoromethoxyphenyl-3-(1-propionylpiperidin-4-yl)urea (TPPU), renal inflammation, fibrosis, glomerulosclerosis mouse model ischemia-reperfusion (IRI)-induced...
Objective The complement receptors C5aR1 and C5aR2 serve distinct roles in immune regulation. In kidney transplantation ischemia reperfusion injury (IRI) results rapid activation. this study deficient mice were tested a model of renal an isogenic cross over (ktx) model. Methods IRI was induced by unilateral clipping pedicle for 45 min C5aR1, −/− wild type (WT). Renal morphology, inflammation, peritubular capillary density fibrosis investigated immunohistochemistry. FACS analysis done at d7...
Background: Ischemia reperfusion injury (IRI) causes acute kidney (AKI) and is a relevant complication in major cardiac surgery. In addition AKI frequent solid organ transplantation (tx): after lung tx the incidence 50% heart with 75% even higher. increases morbidity mortality contributes to progression chronic disease (CKD). During complement cascade activated rapidly subsequent myeloid cells infiltrate kidney. The infiltrating as well resident express receptors by C3a C5a. this study, we...
Objective – Ischemia reperfusion injury (IRI) leads to acute kidney (AKI) after major surgery and contributes progressiverenal fibrosis. Activation of the RAAS system hypertension arecharacteristics AKI. However, most frequently used mouse strain (C57Bl6) in IRI research lack blood pressure elevation IRI. WecomparedCD1 mice with C57Bl/6 model induced AKI effects activation. Methods was by clamping renal pedicle for 35 min. Renal function, glomerular filtration rate (GFR) flow (RBF) were...
Objective Ischemia reperfusion injury (IRI) causes acute kidney (AKI) which is a relevant complication in major cardiac surgery. IRI rapid complement activation and C5a signals via the C5aR like 2 (C5L2) receptor. Currently, little known about differences between these two receptors. Methods was induced C5L2 deficient wild type mice (WT) by unilateral clipping of right renal pedicle for 45 min. Renal blood flow (RBF) glomerular filtration rate (GFR) were assessed inulin PAH clearance....
Die Aktivierung des Komplementsystems spielt in der frühen Entzündungsreaktion und Pathogenese akuten Nierenversagens (ANV) eine wichtige Rolle. Ziel war es, den Einfluss Komplementrezeptors C5L2 auf die Entstehung ANV nach Ischämie-Reperfusionsschaden mittels nicht-invasiver renaler Perfusionsmessung im MRT (Arterial Spin Labeling = ASL) Vergleich zu operativen Messungen von glomerulärer Filtrationsrate (GFR) renalem Plasmafluss (RPF) histologischem Befund untersuchen.