A5102826231- Malaria Research and Control
- Immune Cell Function and Interaction
- Trypanosoma species research and implications
- Mosquito-borne diseases and control
- Research on Leishmaniasis Studies
- Amoebic Infections and Treatments
- Plant nutrient uptake and metabolism
- T-cell and B-cell Immunology
- Complement system in diseases
- Plant Molecular Biology Research
- Parasites and Host Interactions
- Legume Nitrogen Fixing Symbiosis
- Parasitic Infections and Diagnostics
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Bone Tissue Engineering Materials
- Estrogen and related hormone effects
- Invertebrate Immune Response Mechanisms
- Pancreatitis Pathology and Treatment
- Plant Reproductive Biology
- Toxin Mechanisms and Immunotoxins
- Neuroinflammation and Neurodegeneration Mechanisms
- Photosynthetic Processes and Mechanisms
- Diagnosis and treatment of tuberculosis
- HER2/EGFR in Cancer Research
Bernhard Nocht Institute for Tropical Medicine
2016-2025
Virginia Commonwealth University
2019-2024
Research Center Borstel - Leibniz Lung Center
2024
German Center for Infection Research
2019-2022
Jacobs (United States)
2021
University of Illinois Urbana-Champaign
1991-2018
Metropolitan University
2011
Rega Institute for Medical Research
2009
KU Leuven
2009
EpiVax (United States)
2009
Macrophages are functionally heterogeneous cells essential for apoptotic cell clearance. Apoptotic defined by homogeneous characteristics, ignoring their original lineage identity. We found that in an interleukin-4 (IL-4)-enriched environment, the sensing of neutrophils macrophages triggered tissue remodeling signature. Engulfment hepatocytes promoted a tolerogenic phenotype, whereas phagocytosis T had little effect on IL-4-induced gene expression. In mouse model parasite-induced pathology,...
We previously observed decreased p53 immunostaining over time in paraffin-embedded sections of ductal carcinoma situ the breast women; these had been stored on slides at room temperature. This observation suggests that slide storage adversely affects p53-immuno-staining intensity and could result spurious negative staining for patient samples. The goals this study were to determine course factors influencing loss immunoreactivity investigate whether a similar reactivity occurs with other...
Crop loss due to soil salinization is an increasing threat agriculture worldwide. This review provides overview of cellular and physiological mechanisms in plant responses salt. We place a time- tissue-dependent context ...Read More
Nodulation (nod) genes are required for Rhizobium meliloti to invade and stimulate nodule formation in its host, alfalfa. We have established the DNA sequence of nodD, nodA, nodB, which part a gene cluster located 20 kb downstream nifHDK on R. pSym megaplasmid. The nodD open reading frame (308 amino acids) reads from proximal toward distal nifHDK, divergently nodA (196 aa) nodB (217 aa). These two read proximal, just upstream previously defined nodC. Fourteen Tn5 insertion sites been...
Infection of alfalfa by the soil bacterium Rhizobium meliloti proceeds deformation root hairs and bacterial invasion host tissue way an infection thread. We studied 8.7-kilobase (kb) segment R. megaplasmid, which contains genes required for infection. Site-directed Tn5 mutagenesis was used to examine this fragment nodulation genes. A total 81 strains with mapped insertions in 8.7-kb were evaluated phenotype on plants; 39 defined a 3.5-kb containing functions. Of these mutants, 37 completely...
IL-10 is a prototypical anti-inflammatory cytokine, which fundamental to the maintenance of immune homeostasis, especially in intestine. There an assumption that cells producing have immunoregulatory function. However, here we report IL-10-producing CD4+ T are phenotypically and functionally heterogeneous. By combining single cell transcriptome functional analyses, identified subpopulation Foxp3neg displays regulatory activity unlike other cells, unexpectedly pro-inflammatory. The...
In order to identify molecules that might be responsible for the difference in pathogenicity between two closely related protozoan parasites Entamoeba histolytica and dispar , we focussed on cysteine proteinases because this class of enzymes has been considered important pathogenic tissue destruction. By screening a genomic library derived from an E. isolate, total six distinct genes ( ehcp1–ehcp6 ) encoding typical prepro‐forms were identified which differed each other by 40% 85% their...
The mitotic cell cycle of yeast and animal cells is regulated by the cdc2 gene its product, p34 protein kinase, other components MPF or histone H1 kinase complex. We present evidence that cdc2, p34, a also exist in higher plants. Protein extracts from 10 plant species surveyed display 34-kDa component recognized monoclonal antibody directed against an evolutionarily conserved epitope fission p34. Nondenatured Pisum sativum (garden pea) tissues were fractionated gel filtration,...
The induction of proliferation and differentiation in cultured mammalian cells is mediated by a cascade protein phosphorylations. A key enzyme this signaling pathway mitogen-activated (MAP) kinase (or ERK, extracellular signal-regulated kinase). We report the recovery full-length cDNA clone encoding MAP from alfalfa. have named 44-kD encoded MsERK1. Recombinant MsERK1 (rMsERK1), when overexpressed Escherichia coli, recognized antibodies raised against kinases rat, Xenopus, sea star...
The innate immune response is supposed to play an essential role in the control of amebic liver abscess (ALA), a severe form invasive amoebiasis due infection with protozoan parasite Entamoeba histolytica. In mouse model for disease, we previously demonstrated that Jα18-/- mice, lacking invariant natural killer T (iNKT) cells, suffer from more development. Here show specific activation iNKT cells using α-galactosylceramide (α-GalCer) induces significant reduction sizes ALA lesions, whereas...
We demonstrate strong-to-perfect absorption across a wide range of mid-infrared wavelengths (5-12µm) using two-layer system consisting heavily-doped silicon and thin high-index germanium dielectric layer. spectral control the resonance by varying thickness The is shown to be largely polarization-independent angle-invariant. Upon heating, we observe selective thermal emission from our materials. Experimental data compared an analytical model structures with strong agreement.
Amebic liver abscess (ALA), a parasitic disease due to infection with the protozoan Entamoeba histolytica, occurs age and gender dependent strong preferences for adult males. Using mouse model ALA similar male bias disease, we have investigated role of female sexual hormones provide evidence contribution testosterone. Removal testosterone by orchiectomy significantly reduced sizes abscesses in mice, while substitution increased development mice. Activation natural killer T (NKT) cells, which...
Amebic liver abscess (ALA) is a focal destruction of tissue due to infection by the protozoan parasite Entamoeba histolytica (E. histolytica). Host damage attributed mainly pathogenicity factors, but massive early accumulation mononuclear cells, including neutrophils, inflammatory monocytes and macrophages, at site raises question whether these cells also contribute damage. Using highly selective depletion strategies cell-specific knockout mice, relative contribution innate immune cell...
To escape expulsion by their host's immune system, pathogenic nematodes exploit regulatory pathways that are intrinsic parts of the mammalian such as T cells (Tregs). Using depletion Treg mice, we showed Foxp3(+) numbers increased rapidly during infection with nematode Strongyloides ratti. Transient Tregs first days led to dramatically reduced worm burden and larval output, without aggravation pathology. The transient absence primary did not interfere generation protective memory. Depletion...
In acute Plasmodium falciparum (P. falciparum) malaria, the pro- and anti-inflammatory immune pathways must be delicately balanced so that parasitemia is controlled without inducing immunopathology. An important mechanism to fine-tune T cell responses in periphery induction of coinhibitory receptors such as CTLA4 PD1. However, their role infections P. malaria remains poorly understood. To test whether modulate CD4+ functions blood samples were obtained from patients with treated Germany....
Coronavirus disease 2019 (COVID-19) which is caused by the novel SARS-CoV-2 virus a severe flu-like illness associated with hyperinflammation and immune dysfunction. The induces strong T B cell response but little known about pathology of this viral infection. Acute Plasmodium falciparum malaria also causes acute clinical characterized due to production pro-inflammatory cytokines massive activation cells. In malaria, cells express variety co-inhibitory receptors might be consequence their...
Major pathogenic functions of Entamoeba histolytica involved in destruction host tissues are the degradation extracellular matrix proteins mediated by secreted cysteine proteinases and contact‐dependent killing cells via membrane‐active factors. A soluble protein with an affinity for membranes was purified from amoebic extracts to apparent homogeneity. N‐terminal sequencing subsequent molecular cloning factor revealed that it is a member proteinase family E. , which we termed CP5. Further...
Listeriolysin O (LLO) binds to cholesterol‐containing membranes in which it oligomerizes form pores. Preincubation of the toxin with cholesterol is known inhibit haemolysis, whereas oxidized has no inhibitory effect. Using immunoblot analyses and flow cytometry we demonstrate that preincubation does not influence binding listeriolysin–cholesterol complex red blood cells, eukaryotic cells or artificial membranes. Lytic activity membrane‐bound LLO inactivated by can be restored enzymatic...
Amebic liver abscess (ALA) is the most common extraintestinal manifestation of human infection by enteric protozoan parasite Entamoeba histolytica. In contrast to intestinal infection, ALA greatly predominates in males but rare females. Since humans are only relevant host for E. histolytica, experimental studies concerning this sexual dimorphism have been hampered lack a suitable animal model. By serial passage cultured histolytica trophozoites gerbils and mice, we generated amebae which...
Trypanosoma cruzi causes a suppression of the immune system leading to persistence in host cells. The trans-sialidase expressed by T. is major virulence factor and transfers sialic acid from glycoconjugates mucin-like molecules on parasite. Here we demonstrate that these sialylated structures play role immunosuppression. We used two strains, whose TS activity correlated with their pathogenicity. Tulahuen strain, characterized high efficiently infected mice, whereas Tehuantepec strain showing...