A5082732480- Phagocytosis and Immune Regulation
- Immune Cell Function and Interaction
- Immune cells in cancer
- Autophagy in Disease and Therapy
- COVID-19 Clinical Research Studies
- Erythrocyte Function and Pathophysiology
- Pancreatitis Pathology and Treatment
- Nicotinic Acetylcholine Receptors Study
- Neuroinflammation and Neurodegeneration Mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune responses and vaccinations
- Ion channel regulation and function
- Immune Response and Inflammation
- Apelin-related biomedical research
- Cancer Immunotherapy and Biomarkers
- Inflammasome and immune disorders
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Asthma and respiratory diseases
- CAR-T cell therapy research
- Cell death mechanisms and regulation
- Receptor Mechanisms and Signaling
- Adenosine and Purinergic Signaling
- SARS-CoV-2 and COVID-19 Research
- Liver physiology and pathology
Yale University
2016-2025
Centre Méditerranéen de Médecine Moléculaire
2024
IE University
2023
University School
2023
Technical University of Munich
2019
Institut Curie
2019
Yale Cancer Center
2017
Northwestern University
2017
Columbia University
2017
University of New Haven
2014
We report the cloning and characterization of rat α10, a previously unidentified member nicotinic acetylcholine receptor (nAChR) subunit gene family. The protein encoded by α10 nAChR is most similar to α9 nAChR, both genes are transcribed in adult mechanosensory hair cells. Injection Xenopus laevis oocytes with cRNA alone or pairwise combinations either α2-α6 β2-β4 cRNAs yielded no detectable ACh-gated currents. However, coinjection resulted appearance an unusual subtype. Compared homomeric...
Local macrophage clean-up Infection, especially by helminths or bacteria, can cause tissue damage (see the Perspective Bouchery and Harris). Minutti et al. studied mouse models of helminth infection fibrosis. They expressed surfactant protein A (a member complement component C1q family) in lung, which enhanced interleukin-4 (IL-4)-mediated proliferation activation alveolar macrophages. This accelerated clearance reduced lung injury. In peritoneum, boosted for liver repair after bacterial...
Tissue-resident macrophages display varying phenotypic and functional properties that are largely specified by their local environment. One of these functions, phagocytosis, mediates the natural disposal billions cells, but its mechanisms consequences within living tissues poorly defined. Using a parabiosis-based strategy, we identified isolated from multiple as they phagocytosed blood-borne cellular material. Phagocytosis was circadianally regulated mediated distinct repertoires receptors,...
Cell death is prevalent throughout life; however, the coordinated interactions and roles of phagocytes during corpse removal in live brain are poorly understood. We developed photochemical viral methodologies to induce single cells combined this with intravital optical imaging. This approach allowed us track multicellular phagocytic precise spatiotemporal resolution. Astrocytes microglia engaged dying neurons an orchestrated synchronized fashion. Each glial cell played specialized roles:...
Clinical benefits of reperfusion after myocardial infarction are offset by maladaptive innate immune cell function, and therapeutic interventions lacking.We sought to test the significance phagocytic clearance resident recruited phagocytes ischemia reperfusion.In humans, we discovered that clinical led significant elevation soluble form MerTK (myeloid-epithelial-reproductive tyrosine kinase; ie, MER), a critical biomarker compromised phagocytosis macrophages. In reperfused mice, macrophage...
In concert with their phagocytic activity, macrophages are thought to regulate the host immunometabolic responses primarily via ability produce specific cytokines and metabolites. Here, we show that IL-4-differentiated, M2-like secrete IGF1, a hormone previously be exclusively produced from liver. Ablation of IGF1 receptors myeloid cells reduced phagocytosis, increased in adipose tissue, elevated adiposity, lowered energy expenditure, led insulin resistance mice fed high-fat diet. The...
Macrophages are functionally heterogeneous cells essential for apoptotic cell clearance. Apoptotic defined by homogeneous characteristics, ignoring their original lineage identity. We found that in an interleukin-4 (IL-4)-enriched environment, the sensing of neutrophils macrophages triggered tissue remodeling signature. Engulfment hepatocytes promoted a tolerogenic phenotype, whereas phagocytosis T had little effect on IL-4-induced gene expression. In mouse model parasite-induced pathology,...
Type I interferons (IFNs) are pleiotropic cytokines with antiviral and immunomodulatory properties. The immunosuppressive actions of type IFNs poorly understood, but IFN-mediated suppression TNFα production has been implicated in the regulation inflammation contributes to effectiveness treatment certain autoimmune inflammatory diseases. In this study, we investigated mechanisms by which suppress induction immune complexes, Fc receptors, Toll-like receptors. Suppression was mediated...
Macrophages are a source of both proinflammatory and restorative functions in damaged tissue through complex dynamic phenotypic changes. Here, we sought to determine whether monocyte-derived macrophages (MDMs) contribute recovery after acute sterile brain injury. By profiling the transcriptional dynamics MDMs murine experimental intracerebral hemorrhage (ICH), found robust changes infiltrating over time demonstrated that essential for optimal hematoma clearance neurological recovery. Next,...
Tyro3, Axl, and Mertk (TAM) receptors are candidate entry for infection with the Zika virus (ZIKV), an emerging flavivirus of global public health concern. To investigate requirement TAM ZIKV infection, we used several routes viral inoculation compared replication in wild-type versus Axl-/-, Mertk-/-, Axl-/-Mertk-/-, Axl-/-Tyro3-/- mice various organs. Pregnant non-pregnant treated interferon-α-receptor (IFNAR)-blocking (MAR1-5A3) antibody infected subcutaneously showed no reliance on TAMs...
The receptor tyrosine kinases Axl and Mer, belonging to the Tyro3, Mer (TAM) family, are expressed in a number of tumor cells have well-characterized oncogenic roles. therapeutic targeting these is considered an anticancer strategy, various inhibitors currently under development. At same time, dendritic macrophages essential function limiting inflammation. Inflammation enabling characteristic multiple cancer hallmarks. These contrasting anti-inflammatory functions posit potential paradox...
Background & AimsLiver fibrosis, an important health concern associated to chronic liver injury that provides a permissive environment for cancer development, is characterized by accumulation of extracellular matrix components mainly derived from activated hepatic stellate cells (HSCs). Axl, receptor tyrosine kinase and its ligand Gas6, are involved in cell differentiation, immune response carcinogenesis.MethodsHSCs were obtained WT Axl−/− mice, treated with recombinant Gas6 protein (rGas6),...