A5060370342- Genetic and Kidney Cyst Diseases
- Hedgehog Signaling Pathway Studies
- Hippo pathway signaling and YAP/TAZ
- Microtubule and mitosis dynamics
- Wnt/β-catenin signaling in development and cancer
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Axon Guidance and Neuronal Signaling
- Neurogenesis and neuroplasticity mechanisms
- Congenital heart defects research
- Pluripotent Stem Cells Research
- Cellular Mechanics and Interactions
- Genetic Syndromes and Imprinting
- Protein Degradation and Inhibitors
- Mitochondrial Function and Pathology
- Muscle Physiology and Disorders
- Nerve injury and regeneration
- Cancer Cells and Metastasis
- Liver physiology and pathology
- Pancreatitis Pathology and Treatment
- Congenital Ear and Nasal Anomalies
- 3D Printing in Biomedical Research
- Cellular transport and secretion
- Fetal and Pediatric Neurological Disorders
- Phagocytosis and Immune Regulation
Institut de Biologia Molecular de Barcelona
2015-2023
Barcelona Institute for Science and Technology
2021
Institute for Bioengineering of Catalonia
2021
Institut d'Investigacions Biomèdiques de Barcelona
2009-2015
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2014
Background & AimsLiver fibrosis, an important health concern associated to chronic liver injury that provides a permissive environment for cancer development, is characterized by accumulation of extracellular matrix components mainly derived from activated hepatic stellate cells (HSCs). Axl, receptor tyrosine kinase and its ligand Gas6, are involved in cell differentiation, immune response carcinogenesis.MethodsHSCs were obtained WT Axl−/− mice, treated with recombinant Gas6 protein (rGas6),...
Cerebellar granular neuronal precursors (CGNPs) proliferate in response to the mitogenic activity of Sonic hedgehog (Shh), and this proliferation is negatively regulated by activation cAMP-dependent protein kinase (PKA). In basal state, PKA catalytic subunits (C-PKA) are inactive because their association with regulatory (R-PKA). As level cAMP increases, it binds R-PKA, displacing thereby activating C-PKA. Here we report that, presence Shh, C-PKA accumulates at cilium base proliferative...
Protein kinase A (PKA) accumulates at the base of cilium where it negatively regulates Hedgehog (Hh) pathway. Although PKA activity is essentially controlled by cAMP produced Adenylyl Cyclases (AC), influence these enzymes on Hh pathway remains unclear. Here we show that AC5 and AC6 are two isoforms most strongly expressed in cerebellar granular neuron precursors (CGNPs) while over-expression represses pathway, their knockdown results activation this CGNPs embryonic neural tube (NT). Indeed,...
Proliferation of cerebellar granular neuronal precursors (CGNPs) is mediated by Sonic Hedgehog (Shh), which activates the Patched and Smoothened (Smo) receptor complex. Although its protein sequence suggests that Smo a G coupled (GPCR), evidence this utilizes heterotrimeric proteins as downstream effectors controversial. In Drosophila, Gα(i) required for (Hh) activity, but involvement in vertebrate Shh signaling has not yet been established. Here, we show Shh-induced proliferation rat CGNPs...
Class II HLH proteins heterodimerize with class I HLH/E to regulate transcription. Here, we show that E sharpen neurogenesis by adjusting the neurogenic strength of distinct proneural proteins. We find inhibiting BMP signaling or its target ID2 in chick embryo spinal cord, impairs neuronal production from progenitors expressing ATOH1/ASCL1, but less severely NEUROG1/2/PTF1a. this context-dependent response result differential modulation proteins’ activity synergize when acting on CAGSTG...
Here, we show that, in the developing spinal cord, after early Wnt-mediated Tcf transcription activation that confers dorsal identity to neural stem cells, neurogenesis redirects β-catenin from adherens junctions nucleus stimulate Tcf-dependent a Wnt-independent manner. This new activity regulates genes implicated several aspects of contralateral axon growth, including guidance and adhesion. Using live imaging ex-vivo chick tube, showed nuclear accumulation rise both initiate before...
Vinculin is an actin-binding protein (ABP) that strengthens the connection between actin cytoskeleton and adhesion complexes. It binds to β-catenin/N-cadherin complexes in apical adherens junctions (AJs), which maintain cell-to-cell adhesions, talin/integrins focal adhesions (FAs) attach cells basal membrane. Here, we demonstrate β-catenin targets vinculin AJs centrosome embryonic neural tube (NT). Suppression of slows down basal-to-apical part interkinetic nuclear migration (BAINM), arrests...
The neural tube forms when stem cells arrange into a pseudostratified, single-cell–layered epithelium, with marked apico-basal polarity, and in which adherens junctions (AJs) concentrate the subapical domain. We previously reported that sustained β-catenin expression promotes formation of enlarged apical complexes (ACs), enhancing although mechanism through this occurs remained unclear. Here, we show interacts phosphorylated pro-N-cadherin early its transit Golgi apparatus, promoting...
Protein kinase A (PKA) accumulates at the base of cilium where it negatively regulates Hedgehog (Hh) pathway. Although PKA activity is essentially controlled by cAMP produced adenylyl cyclases, influence these enzymes on Hh pathway remains unclear. Here, we show that cyclase 5 and 6 (AC5 AC6, also known as ADCY5 ADCY6, respectively) are two isoforms most strongly expressed in cerebellar granular neuron precursors (CGNPs). We found overexpression AC5 AC6 represses, whereas their knockdown...
Cerebellar granular neuronal precursors (CGNPs) proliferate in response to the mitogenic activity of Sonic hedgehog (Shh), and this proliferation is negatively regulated by activation cAMP-dependent protein kinase (PKA). In basal state, PKA catalytic subunits (C-PKA) are inactive because their association with regulatory (R-PKA). As level cAMP increases, it binds R-PKA, displacing thereby activating C-PKA. Here we report that, presence Shh, C-PKA accumulates at cilium base proliferative...
SUMMARY STATEMENT During neural tube development, PI3K pathway promotes cell survival and provides the apical-basal navigation clues that define final location of neurons in epithelium. Phosphatidylinositol 3-kinases (PI3Ks) are signal transducers many biological processes. Class 1A PI3Ks hetero dimers formed by a regulatory catalytic subunit. We have used developing chicken (NT) to study roles played during process proliferation differentiation. Notably, we observed addition its well...
Abstract Basic HLH proteins heterodimerize with class I HLH/E to promote transcription. Here we show that E differentially co-operate proneural bHLH transcription factors sharpening their neurogeneic activity. We find inhibiting BMP signaling or its target ID2, in the chick embryo spinal cord, impairs neuronal production from progenitors expressing ATOH1/ASCL1, but less severely NEUROG1/2/PTF1a. define mechanisms of this differential response as a dual co-operation proteins. synergize when...
SUMMARY Neural stem cells (NSCs) form a pseudostratified, single-cell layered epithelium with marked apico-basal polarity. In these cells, β-Catenin associates classic cadherins in order to the apical adherens junctions (AJs). We previously reported that oncogenic forms of (sβ-Catenin) maintain neural precursors as progenitors, while also enhancing their polarization and adhesiveness, thereby limiting malignant potential. Here we show can bind phosphorylated Pro-N-Cadherin, promoting...