A5091877247- Hearing, Cochlea, Tinnitus, Genetics
- Nicotinic Acetylcholine Receptors Study
- Receptor Mechanisms and Signaling
- Hearing Loss and Rehabilitation
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Vestibular and auditory disorders
- Photoreceptor and optogenetics research
- Biochemical Analysis and Sensing Techniques
- Connexins and lens biology
- Ion Channels and Receptors
- Cholinesterase and Neurodegenerative Diseases
- Neuroscience of respiration and sleep
- Neural dynamics and brain function
- Neuroendocrine regulation and behavior
- Marine animal studies overview
- Olfactory and Sensory Function Studies
- Noise Effects and Management
- Animal Vocal Communication and Behavior
- Neurotransmitter Receptor Influence on Behavior
- Adipose Tissue and Metabolism
- Pharmacological Receptor Mechanisms and Effects
- RNA regulation and disease
- ATP Synthase and ATPases Research
- Bat Biology and Ecology Studies
Consejo Nacional de Investigaciones Científicas y Técnicas
2016-2025
Experimental Medicine and Biology Institute
2016-2025
University of Buenos Aires
2015-2025
Instituto de Biomedicina y Genética Molecular de Valladolid
2022-2024
Hospital de Clínicas "José de San Martín"
2023
Argerich Hospital
2023
Fundación Ciencias Exactas y Naturales
1999-2017
University College London
2017
Universidad Nacional del Sur
2017
Institute of Astronomy and Space Physics
2000-2015
We report the cloning and characterization of rat α10, a previously unidentified member nicotinic acetylcholine receptor (nAChR) subunit gene family. The protein encoded by α10 nAChR is most similar to α9 nAChR, both genes are transcribed in adult mechanosensory hair cells. Injection Xenopus laevis oocytes with cRNA alone or pairwise combinations either α2-α6 β2-β4 cRNAs yielded no detectable ACh-gated currents. However, coinjection resulted appearance an unusual subtype. Compared homomeric...
Phantom perception refers to the conscious awareness of a percept in absence an external stimulus. On basis basic neuroscience on and clinical research phantom pain sound, we propose working model for their origin. Sensory deafferentation results high-frequency, gamma band, synchronized neuronal activity sensory cortex. This becomes only if it is connected larger coactivated “(self-)awareness” “salience” brain networks. Through involvement learning mechanisms, associated distress, which turn...
The α9 and α10 nicotinic acetylcholine receptor (nAChR) subunits assemble to form the α9α10 nAChR subtype. This is believed mediate cholinergic synaptic transmission between efferent olivocochlear fibers hair cells of cochlea. In addition and/or expression has been described in dorsal root ganglion neurons, lymphocytes, skin keratinocytes, pars tuberalis pituitary. Specific antagonists that selectively block channel could be valuable tools for elucidating its role these diverse tissues....
Cochlear synaptopathy produced by exposure to noise levels that cause only transient auditory threshold elevations is a condition affects many people and believed contribute poor speech discrimination in noisy environments. These functional deficits hearing, without changes sensitivity, have been called hidden hearing loss (HHL). It has proposed activity of the medial olivocochlear (MOC) system can ameliorate acoustic trauma effects. Here we explore role MOC HHL comparing performance two...
In the mature cochlea, inner hair cells (IHCs) transduce acoustic signals into receptor potentials, communicating to brain by synaptic contacts with afferent fibers. Before onset of hearing, a transient efferent innervation is found on IHCs, mediated nicotinic cholinergic that may contain both α9 and α10 subunits. Calcium influx through activates calcium-dependent (SK2-containing) potassium channels. This inhibitory synapse thought disappear after hearing [after postnatal day 12 (P12)]. We...
Through a study of cloned nicotinic receptors expressed in Xenopus oocytes, we provide evidence that alpha-conotoxin ImI, peptide marine snail toxin induces seizures rodents, selectively blocks subtypes acetylcholine receptors. alpha-Conotoxin ImI homomeric alpha 7 with the highest apparent affinity and 9 8-fold lower affinity. This has no effect on composed 2 beta 2, 3 4 4, or subunit combinations. In contrast to alpha-bungarotoxin, which high for 7, 9, 1 gamma delta receptors, low muscle...
The transduction of sound in the auditory periphery, cochlea, is inhibited by efferent cholinergic neurons projecting from brainstem and synapsing directly on mechanosensory hair cells. One fundamental question neuroscience what role(s) this feedback plays our ability to hear. In present study, we have engineered a genetically modified mouse model which magnitude duration effects are increased, assess consequences manipulation cochlear function. We generated Chrna9L9'T line knockin mice with...
Although homomeric channels assembled from the alpha9 nicotinic acetylcholine receptor (nAChR) subunit are functional in vitro, electrophysiological, anatomical, and molecular data suggest that native cholinergic olivocochlear function is mediated via heteromeric nAChRs composed of both alpha10 subunits. To gain insight into vivo, we examined olivo cochlear innervation null-mutant mice. Electrophysiological recordings postnatal (P) days P8-9 inner hair cells revealed ACh-gated currents...
Tinnitus, the phantom perception of sound, is a prevalent disorder. One in 10 adults has clinically significant subjective tinnitus, and for 1 100, tinnitus severely affects their quality life. Despite unmet clinical need safe effective drug targeting relief, there currently not single FDA-approved on market. The search drugs that target hampered by lack deep knowledge underlying neural substrates this pathology. Recent studies are increasingly demonstrating that, as described other central...
In the present study, we provide evidence that α9 nicotinic acetylcholine receptor (nAChR) shares pharmacological properties with members of Cys-loop family receptors. Thus, type A γ-aminobutyric acid antagonist bicuculline, glycinergic strychnine, and 3 serotonin ICS-205,930 block ACh-evoked currents in α9-injected <i>Xenopus laevis</i> oocytes following rank order potency: strychnine > bicuculline. Block by antagonists was reflected an increase (ACh) EC<sub>50</sub> value, no changes...
The α9 and α10 nicotinic cholinergic subunits assemble to form the receptor that mediates synaptic transmission between efferent olivocochlear fibers hair cells of cochlea. They are latest vertebrate (nAChR) have been cloned, their identification has established a distant early divergent branch within nAChR gene family. subunit serves as “structural” component leading heteromeric α9α10 nAChRs with distinct properties. We now probed stoichiometry recombinant expressed in Xenopus oocytes. made...