A5071497947- Hearing, Cochlea, Tinnitus, Genetics
- Nicotinic Acetylcholine Receptors Study
- Hearing Loss and Rehabilitation
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Biochemical Analysis and Sensing Techniques
- Ion channel regulation and function
- Vestibular and auditory disorders
- Neuroscience and Neural Engineering
- Neuroscience and Music Perception
- Acoustic Wave Phenomena Research
- Olfactory and Sensory Function Studies
- Cholinesterase and Neurodegenerative Diseases
- Animal Vocal Communication and Behavior
Consejo Nacional de Investigaciones Científicas y Técnicas
2007-2023
University of Buenos Aires
2011-2023
Centre National de la Recherche Scientifique
2017
Institut du Cerveau
2017
Inserm
2017
Sorbonne Université
2017
University College London
2014-2015
Experimental Medicine and Biology Institute
2005-2011
Institute of Astronomy and Space Physics
2005
The transduction of sound in the auditory periphery, cochlea, is inhibited by efferent cholinergic neurons projecting from brainstem and synapsing directly on mechanosensory hair cells. One fundamental question neuroscience what role(s) this feedback plays our ability to hear. In present study, we have engineered a genetically modified mouse model which magnitude duration effects are increased, assess consequences manipulation cochlear function. We generated Chrna9L9'T line knockin mice with...
Although homomeric channels assembled from the alpha9 nicotinic acetylcholine receptor (nAChR) subunit are functional in vitro, electrophysiological, anatomical, and molecular data suggest that native cholinergic olivocochlear function is mediated via heteromeric nAChRs composed of both alpha10 subunits. To gain insight into vivo, we examined olivo cochlear innervation null-mutant mice. Electrophysiological recordings postnatal (P) days P8-9 inner hair cells revealed ACh-gated currents...
Significance Locating the source of a sound is critical to survival many species and an important factor in human communication. Auditory spatial cues—differences timing intensity sounds arriving at two ears—are processed by specialized neurons brainstem. The importance these cues varies with frequency. Through vitro recordings we show that biophysical properties brainstem vary their presumed frequency tuning. Using neural modeling demonstrate cell are well suited extract from natural...
In the mammalian inner ear, gain control of auditory inputs is exerted by medial olivocochlear (MOC) neurons that innervate cochlear outer hair cells (OHCs). OHCs mechanically amplify incoming sound waves virtue their electromotile properties while MOC system reduces inhibiting OHC function. How this process orchestrated at synaptic level remains unknown. present study, firing was evoked electrical stimulation in an isolated mouse preparation, postsynaptic responses were monitored whole-cell...
In this study, we report the effects of quinoline derivatives quinine, its optical isomer quinidine, and chloroquine on α9α10-containing nicotinic acetylcholine receptors (nAChRs). The compounds blocked (ACh)-evoked responses in α9α10-injected <i>Xenopus laevis</i> oocytes a concentration-dependent manner, with rank order potency (IC<sub>50</sub> = 0.39 μM) > quinine 0.97 ∼ quinidine 1.37 μM). Moreover, ACh-evoked rat cochlear inner hair cells an IC<sub>50</sub> value 0.13 μM, which is...
In the mammalian auditory system, synapse between efferent olivocochlear (OC) neurons and sensory cochlear hair cells is cholinergic, fast, inhibitory. This mediated by nicotinic α9α10 receptor coupled to activation of SK2 Ca 2+ -activated K + channels that hyperpolarize cell. So far, ion support and/or modulate neurotransmitter release from OC terminals remain unknown. To identify these channels, we used an isolated mouse preparation monitored transmitter synaptic in inner (IHCs) voltage...
The synapse between olivocochlear (OC) neurons and cochlear mechanosensory hair cells is cholinergic, fast, inhibitory. inhibitory sign of this cholinergic accounted for by the activation Ca 2+ -permeable postsynaptic α9α10 nicotinic receptors coupled to opening hyperpolarizing -activated small-conductance type 2 (SK2)K + channels. Acetylcholine (ACh) release at supported both P/Q- N-type voltage-gated calcium channels (VGCCs). Although OC an abundant GABA innervation present along mammalian...
A continuous isoelectric electroencephalogram reflects an interruption of endogenously-generated activity in cortical networks and systematically results a complete dissolution conscious processes. This electro-cerebral inactivity occurs during various brain disorders, including hypothermia, drug intoxication, long-lasting anoxia trauma. It can also be induced therapeutic context, following the administration high doses barbiturate-derived compounds, to interrupt hyper-refractory status...
Gain control of the auditory system operates at multiple levels. Cholinergic medial olivocochlear (MOC) fibers originate in brainstem and make synaptic contacts base outer hair cells (OHCs), final targets several feedback loops from periphery higher-processing centers. Efferent activation inhibits OHC active amplification within mammalian cochlea, through a calcium-permeable α9α10 ionotropic cholinergic nicotinic receptor (nAChR), functionally coupled to calcium activated SK2 potassium...
Improving the electrode-neuron interface to reduce current spread between individual electrodes has been identified as one of main objectives in search for future improvements cochlear-implant performance. Here, we address this problem by presenting a novel stimulation strategy that takes account biophysical properties auditory neurons (spiral ganglion neurons, SGNs) stimulated electrical hearing. This new employs ramped pulse shape, where maximum amplitude is achieved through linear slope...
Cholesterol contributes to neuronal membrane integrity, supports protein clustering and function, facilitates proper signal transduction. Extensive evidence has shown that cholesterol imbalances in the central nervous system occur aging development of neurodegenerative diseases. In this work, we characterize homeostasis inner ear young aged mice as a new unexplored possibility for prevention treatment hearing loss. Our results show levels are reduced during aging, an effect is associated...
Abstract A potential auditory spatial cue, the envelope interaural time difference (ITD ENV ) is encoded in lateral superior olive (LSO) of brainstem. Here, we explore computationally modeled LSO neurons, reflecting behavioral sensitivity to ITD . Transposed tones (half-wave rectified low-frequency tones, frequency-limited, then multiplying a high-frequency carrier) stimulate bilateral auditory-periphery model driving each neuron, where electrical membrane impedance low-pass filters inputs...