A5028142872- Hearing, Cochlea, Tinnitus, Genetics
- Nicotinic Acetylcholine Receptors Study
- Neuroscience and Neuropharmacology Research
- Ion Channels and Receptors
- Hearing Loss and Rehabilitation
- Biochemical Analysis and Sensing Techniques
- Photoreceptor and optogenetics research
- Ion channel regulation and function
- Noise Effects and Management
- Marine animal studies overview
- Autism Spectrum Disorder Research
- Neuroendocrine regulation and behavior
- Neurotransmitter Receptor Influence on Behavior
- Vestibular and auditory disorders
- Connexins and lens biology
- Genetics and Neurodevelopmental Disorders
- Neural dynamics and brain function
- Receptor Mechanisms and Signaling
- Attention Deficit Hyperactivity Disorder
- Neurobiology and Insect Physiology Research
- Behavioral and Psychological Studies
Consejo Nacional de Investigaciones Científicas y Técnicas
2007-2025
Experimental Medicine and Biology Institute
2006-2024
University of Buenos Aires
2012
Abstract The dopamine D4 receptor (D4R) has received considerable interest because of its higher affinity for atypical antipsychotics, the extremely polymorphic nature human gene and genetic association with attention deficit hyperactivity disorder (ADHD). Several efforts have been undertaken to determine D4R expression pattern in brain using immunohistochemistry, binding autoradiography situ hybridization, but overall published results present large discrepancies. Here, we explored an...
Spontaneous electrical activity generated by developing sensory cells and neurons is crucial for the maturation of neural circuits. The full mammalian auditory inner hair (IHCs) depends on patterns spontaneous action potentials during a ‘critical period’ development. intrinsic spiking IHCs can be modulated inhibitory input from cholinergic efferent fibres descending brainstem, which transiently innervate immature IHCs. However, it remains unknown whether this transient to required their...
Cochlear outer hair cells (OHCs) are electromotile and implicated in amplification of responses to sound that enhance sensitivity frequency tuning. They send afferent information through glutamatergic synapses onto type II spiral ganglion neurons (SGNs). These weaker than those from cochlear inner I SGN, suggesting SGNs respond only intense levels. OHCs also receive efferent innervation medial olivocochlear (MOC) neurons. MOC cholinergic yet inhibit due the functional coupling alpha9/alpha10...
The synapse between olivocochlear (OC) neurons and cochlear mechanosensory hair cells is cholinergic, fast, inhibitory. inhibitory sign of this cholinergic accounted for by the activation Ca 2+ -permeable postsynaptic α9α10 nicotinic receptors coupled to opening hyperpolarizing -activated small-conductance type 2 (SK2)K + channels. Acetylcholine (ACh) release at supported both P/Q- N-type voltage-gated calcium channels (VGCCs). Although OC an abundant GABA innervation present along mammalian...
The auditory system in many mammals is immature at birth but precisely organized adults. Spontaneous activity the inner ear plays a critical role guiding this maturation process. This shaped by an efferent pathway that descends from brainstem and makes transient direct synaptic contacts with hair cells. In work, we used α9 cholinergic nicotinic receptor knock-in mouse model (of either sex) enhanced medial (<i>Chrna9L9</i>′<i>T</i>, <i>L9</i>′<i>T</i>) to further understand of olivocochlear...
Abstract Cochlear outer hair cells (OHCs) are electromotile and implicated in mechanisms of amplification responses to sound that enhance sensitivity frequency tuning. They send information the brain through glutamatergic synapses onto a small subpopulation neurons ascending auditory nerve, type II spiral ganglion (SGNs). The OHC SGNs sparse weak, suggesting respond primarily loud possibly damaging levels sound. OHCs also receive innervation from medial olivocochlear (MOC) efferent neurons....
Gain control of the auditory system operates at multiple levels. Cholinergic medial olivocochlear (MOC) fibers originate in brainstem and make synaptic contacts base outer hair cells (OHCs), final targets several feedback loops from periphery higher-processing centers. Efferent activation inhibits OHC active amplification within mammalian cochlea, through a calcium-permeable α9α10 ionotropic cholinergic nicotinic receptor (nAChR), functionally coupled to calcium activated SK2 potassium...
The lateral line (LL) is a sensory system that allows fish and amphibians to detect water currents. LL responsiveness modulated by efferent neurons aid in distinguishing between external self-generated stimuli, maintaining sensitivity relevant cues. One component of the cholinergic, activation which inhibits afferent activity. hair cells (HCs) share structural, functional, molecular similarities with those cochlea, making them popular model for studying human hearing balance disorders....
Abstract Acetylcholine is a key neurotransmitter of the inner ear efferent system. In this study, we identify two novel nAChR subunits in ear: α1 and γ, encoded by Chrna1 Chrng , respectively. situ hybridization shows that messages these are present vestibular cochlear hair cells during early development. expression begin at embryonic stage E13.5 system E17.5 organ Corti. message continues through P7, whereas undetectable post‐natal P6. The γ known as muscle‐type surprisingly expressed which...
During development, inner hair cells (IHCs) in the mammalian cochlea are unresponsive to acoustic stimuli but instead exhibit spontaneous activity. this same period, neurons originating from medial olivocochlear complex (MOC) transiently innervate IHCs, regulating their firing pattern which is crucial for correct development of auditory pathway. Although MOC-IHC a cholinergic synapse, previous evidence indicates widespread presence gamma-aminobutyric acid (GABA) signaling markers, including...
In the mature mammalian cochlea, inner hair cells (IHCs) are mainly innervated by afferent fibers that convey sound information to CNS. During postnatal development, however, medial olivocochlear (MOC) efferent transiently innervate IHCs. The MOC–IHC synapse, functional from day 0 (P0) hearing onset (P12), undergoes dramatic changes in sensitivity acetylcholine (ACh) and expression of key postsynaptic proteins. To evaluate whether there associated properties ACh release during this period,...
The organ of Corti, the auditory mammalian sensory epithelium, contains two types mechanotransducer cells, inner hair cells (IHCs) and outer (OHCs). IHCs are involved in conveying acoustic stimuli to CNS, while OHCs implicated fine tuning amplification sounds. innervated by medial olivocochlear (MOC) cholinergic efferent fibers. functional characteristics MOC–OHC synapse during maturation were assessed electrophysiological pharmacological methods mouse organs Corti at postnatal day 11...
The activation of α9α10 nicotinic cholinergic receptors (nAChRs) present at the synapse between efferent olivocochlear fibres and cochlear hair cells can prevent acoustic trauma. Hence, pharmacological potentiators these could be useful therapeutically. In this work, we characterize ascorbic acid as a positive modulator recombinant nAChRs.ACh-evoked responses were analysed under two-electrode voltage-clamp recordings in Xenopus laevis oocytes injected with α9 α10 cRNAs.Ascorbic potentiated...
Abstract In the mature mammalian cochlea, inner hair cells (IHCs) are mainly innervated by afferent fibers that convey sound information to central nervous system. During postnatal development, however, medial olivocochlear (MOC) efferent transiently innervate IHCs. The MOC-IHC synapse, functional from day (P)0 hearing onset (P12), undergoes dramatic changes in sensitivity acetylcholine (ACh) and expression of key postsynaptic proteins. To evaluate whether there associated properties ACh...
A neural network model of operant conditioning for appetitive and aversive stimuli is proposed. From neurobiological behavioural bases it assumed that animals are able to compute the prediction unconditioned stimulus. The controls learning correct response obtain reward avoid punishment. has as inputs: all conditioned outputs possible responses animal; each one computed by neuron. Based on Hebbian or anti-Hebbian learning, depending prediction, synaptic weights neurons calculated. neuron...
Abstract The auditory system in many mammals is immature at birth but precisely organized adults. Spontaneous activity the inner ear plays a critical role guiding this process. This shaped by an efferent pathway that descends from brainstem and makes transient direct synaptic contacts with hair cells (IHCs). In work, we used α9 cholinergic receptor knock-in mouse model (of either sex) enhanced medial ( Chrna9L9’T , L9’T ) to understand of olivocochlear correct establishment circuits. Wave...
Abstract The lateral line (LL) is a sensory system that allows fish and amphibians to detect water currents. LL responsiveness external stimuli modulated by descending efferent neurons. modulation aids the animal distinguish between self-generated stimuli, maintaining sensitivity relevant cues. One of main components cholinergic, activation which inhibits afferent activity. Since hair cells (HC) share structural, functional molecular similarities with those cochlea, one could propose...