A5011207952- Mast cells and histamine
- Cellular transport and secretion
- Asthma and respiratory diseases
- Neonatal Respiratory Health Research
- Lipid Membrane Structure and Behavior
- Food Allergy and Anaphylaxis Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Neuroscience and Neuropharmacology Research
- Click Chemistry and Applications
- Lung Cancer Diagnosis and Treatment
- Pleural and Pulmonary Diseases
- Polyamine Metabolism and Applications
- Cystic Fibrosis Research Advances
- Platelet Disorders and Treatments
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Ion channel regulation and function
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Immune Cell Function and Interaction
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Respiratory and Cough-Related Research
- Receptor Mechanisms and Signaling
- Erythrocyte Function and Pathophysiology
- Urticaria and Related Conditions
- Eosinophilic Disorders and Syndromes
The University of Texas MD Anderson Cancer Center
2012-2024
Okayama University
2022
Pulmonary Associates
2005-2018
Hospital Nacional Cayetano Heredia
2013
Universidad Peruana Cayetano Heredia
2013
Texas A&M Health Science Center
2013
Baylor College of Medicine
1998-2012
Michael E. DeBakey VA Medical Center
2012
Bloodworks Northwest
2012
Tianjin Medical University General Hospital
2012
Biochemical and genetic data suggest that synaptotagmin-2 functions as a Ca 2+ sensor for fast neurotransmitter release in caudal brain regions, but animals and/or synapses lacking have not been examined. We now generated mice which the 5′ end of gene was replaced by lacZ. Using β-galactosidase marker, we show that, consistent with previous studies, is widely expressed spinal cord, brainstem, cerebellum, additionally present selected forebrain neurons, including most striatal neurons some...
Although it has been shown that mast cell-deficient mice have diminished innate immune responses against bacteria, the most important immunoprotective factors secreted from activated cells not identified. Mouse cell protease 6 is a tetramer-forming tryptase. This serine abundant in secretory granules and exocytosed upon bacterial challenge. Here we described generation of protease-6-null mouse. Our discovery lacking this neutral cannot efficiently clear Klebsiella pneumoniae their peritoneal...
Mucus hypersecretion contributes to morbidity and mortality in many obstructive lung diseases. Gel-forming mucins are the chief glycoprotein components of airway mucus, elevated expression these during mucous metaplasia precedes hypersecretory phenotype. Five orthologous genes (MUC2, MUC5AC, MUC5B, MUC6, MUC19) encode mammalian gel-forming mucin family, several have been implicated asthma, cystic fibrosis, chronic pulmonary disease pathologies. However, absence a comprehensive analysis,...
Abstract Although mast cells (MCs) often are abundant in the synovial tissues of patients with rheumatoid arthritis, contribution MCs to joint inflammation and cartilage loss remains poorly understood. MC-restricted tryptase/heparin complexes have proinflammatory activity, significant amounts human tryptase β (hTryptase-β) present arthritis fluid. Mouse MC protease-6 (mMCP-6) is ortholog hTryptase-β, this serine protease synovium arthritic mice. We now report that C57BL/6 (B6) mice lacking...
Patients with inflammatory bowel disease (IBD) have increased numbers of human tryptase-β (hTryptase-β)-positive mast cells (MCs) in the gastrointestinal tract. The amino acid sequence mouse cell protease (mMCP)-6 is most similar to that hTryptase-β. We therefore hypothesized this mMCP, or related tryptase mMCP-7, might a prominent proinflammatory role experimental colitis. dextran sodium sulfate (DSS) and trinitrobenzene sulfonic (TNBS) colitis models were used evaluate differences between...
The lungs are a common site of serious infection in both healthy and immunocompromised subjects, the most likely route delivery bioterror agent. Since airway epithelium shows great structural plasticity response to inflammatory stimuli, we hypothesized it might also show functional plasticity.To test inducibility lung defenses against bacterial challenge.Mice were treated with an aerosolized lysate ultraviolet-killed nontypeable (unencapsulated) Haemophilus influenzae (NTHi), then challenged...
Mast cells (MCs) contribute to the formation of abdominal aortic aneurysms (AAAs) by producing biologically active mediators. Tryptase is most abundant MC granule protein and participates in activation, protease maturation, leukocyte recruitment, angiogenesis-all processes critical AAA pathogenesis.To test hypothesis that tryptase directly formation.Immunohistochemistry demonstrated enhanced staining media adventitia human mouse lesions. Serum levels correlated significantly with annual...
Synaptotagmins (Syts) I and II are believed to act as Ca2+ sensors in the control of neurotransmission. Here we demonstrate that mast cells express Syt their lysosomal fraction. We further show activation by either aggregation FcεRI or ionophores results exocytosis lysosomes, addition well documented secretory granules. directly regulates exocytosis, whereby overexpression inhibited Ca2+-triggered release processed form cathepsin D, whereas suppression expression markedly potentiated this...
Abstract Objective Increased numbers of mast cells (MCs) that express β tryptases bound to heparin have been detected in the synovium patients with rheumatoid arthritis (RA). The corresponding mice are mouse MC protease 6 (mMCP‐6) and mMCP‐7. Although MCs implicated RA some animal models arthritis, no direct evidence for a MC‐restricted tryptase pathogenesis inflammatory has shown. We created transgenic lack different combinations mMCP‐6 mMCP‐7, evaluate roles tryptase–heparin complexes an...
A second-degree epidermal scald burn in mice elicits an inflammatory response mediated by natural IgM directed to nonmuscle myosin with complement activation that results ulceration and scarring. We find such injury is associated early mast cell (MC) degranulation absent WBB6F1-Kit(W)/Kit(Wv) mice, which lack MCs a context of other defects due mutation the Kit receptor. To address further MC role, we used transgenic strains normal lineage development deficiency specific secretory granule...
Platelet hyperactivity induced by inflammation is a known risk factor for atherosclerosis and thrombosis, but its underlying mechanisms remain poorly understood.The signal transducer activator of transcription 3 (STAT3) was activated in collagen-stimulated platelets. Activated STAT3 served as protein scaffold to facilitate the catalytic interaction between kinase Syk (spleen tyrosine kinase) substrate PLCγ2 enhance collagen-induced calcium mobilization platelet activation. The same with...
Spontaneous electrical activity generated by developing sensory cells and neurons is crucial for the maturation of neural circuits. The full mammalian auditory inner hair (IHCs) depends on patterns spontaneous action potentials during a ‘critical period’ development. intrinsic spiking IHCs can be modulated inhibitory input from cholinergic efferent fibres descending brainstem, which transiently innervate immature IHCs. However, it remains unknown whether this transient to required their...
MUC5AC (mucin 5AC, oligomeric gel-forming) and MUC5B 5B, are the predominant secreted polymeric mucins in mammalian airways. They contribute differently to pathogenesis of various muco-obstructive interstitial lung diseases, their genes separately regulated, but whether they packaged together or separate secretory granules is not known.
Mast cell degranulation is a highly regulated, calcium-dependent process, which important for the acute release of inflammatory mediators during course many pathological conditions. We previously found that Synaptotagmin-2, calcium sensor in neuronal exocytosis, was expressed mast line. postulated this protein may be involved control cell-regulated and we generated Synaptotagmin-2 knock-out mice to test our hypothesis. cells from mutant animal conferred an abnormally decreased passive...