A5056643989- Cell Image Analysis Techniques
- Mosquito-borne diseases and control
- Asthma and respiratory diseases
- Viral Infections and Vectors
- Advanced Fluorescence Microscopy Techniques
- Cancer, Hypoxia, and Metabolism
- Neonatal Respiratory Health Research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Advanced Electron Microscopy Techniques and Applications
- T-cell and B-cell Immunology
- Mathematical Biology Tumor Growth
- Corneal surgery and disorders
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Protist diversity and phylogeny
- Marine Invertebrate Physiology and Ecology
- Photoreceptor and optogenetics research
- Planarian Biology and Electrostimulation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Traumatic Brain Injury and Neurovascular Disturbances
University of Pittsburgh
2016-2025
Biolog (United States)
2021
Children's National
2009-2016
University of Colorado Denver
2013-2016
Colorado School of Public Health
2015
Denver Health Medical Center
2015
Pennsylvania State University
2008-2012
George Washington University
2005-2011
Georgetown University
2011
Hershey (United States)
2010-2011
A growing community is constructing a next-generation file format (NGFF) for bioimaging to overcome problems of scalability and heterogeneity. Organized by the Open Microscopy Environment (OME), individuals institutes across diverse modalities facing these have designed specification process (OME-NGFF) address needs. This paper brings together wide range those members describe cloud-optimized itself-OME-Zarr-along with tools data resources available today increase FAIR access remove barriers...
Abstract The main regulators of leukocyte trafficking during inflammatory responses are chemokines. However, another class recently identified chemotactic agents is extracellular cyclophilins, the proteins mostly known as receptors for immunosuppressive drug, cyclosporine A. Cyclophilins can induce chemotaxis in vitro and have been detected at elevated levels inflamed tissues, suggesting that they might contribute to responses. We CD147 signaling receptor cyclophilin In current study we...
Dengue virus (DENV), a flavivirus of global importance, is transmitted to humans by mosquitoes. In this study, we developed in vitro and vivo models saliva-mediated enhancement DENV infectivity. Serine protease activity Aedes aegypti saliva augmented infectivity proteolyzing extracellular matrix proteins, thereby increasing viral attachment heparan sulfate proteoglycans inducing cell migration. A serine inhibitor reduced vivo, marked 100-fold reduction load murine lymph nodes. screen...
ABSTRACT Engineered alphavirus vectors expressing reporters of infection have been used for a number years due to their relatively low costs analysis virus replication and the capacity utilize imaging systems longitudinal measurements growth within single animals. In general, these derived from Old World alphaviruses using second viral subgenomic promoter express transgenes, placed either immediately after nonstructural proteins or at 3′ end coding sequences. However, relevance natural...
Aedes aegypti is the primary vector of several medically relevant arboviruses including dengue virus (DENV) types 1–4. Ae. transmits DENV by inoculating virus-infected saliva into host skin during probing and feeding. contains over one hundred unique proteins these have diverse functions, facilitating blood Previously, we showed that salivary gland extracts (SGEs) enhanced dissemination to draining lymph nodes. In contrast, HPLC-fractionation revealed some SGE components inhibited infection....
Vascularization plays a critical role in organ maturation and cell type development. Drug discovery, mimicry, ultimately transplantation hinge on achieving robust vascularization of vitro engineered organs. Here, focusing human kidney organoids, we overcame this hurdle by combining induced pluripotent stem (iPSC) line containing an inducible ETS translocation variant 2 (ETV2) (a transcription factor playing endothelial development) that directs differentiation vitro, with non-transgenic iPSC...
To meet the high energy demands of brain function, cerebral blood flow (CBF) parallels changes in neuronal activity by a mechanism known as neurovascular coupling (NVC). However, which neurons play role mediating NVC is not well understood. Here, we identify mice and humans specific population cortical GABAergic that co-express nitric oxide synthase tachykinin receptor 1 (Tacr1). Through whole-tissue clearing, demonstrate Tacr1 extend local long-range projections across functionally...
Airway inflammation and mucus hypersecretion/overproduction/obstruction are pathophysiological characteristics of cystic fibrosis, asthma, chronic obstructive pulmonary disease. Up-regulation airway mucin genes by inflammatory/immune response mediators is one the major contributors to overproduction. IL-8, a potent proinflammatory mediator neutrophil chemoattractant, present at high levels in secretions such patients. In this study, effects IL-8 on expression two genes, MUC5AC MUC5B, were...
A gold standard of antiviral vaccination has been the safe and effective live-attenuated 17D-based yellow fever virus (YFV) vaccines. Among more than 500 million vaccinees, only a handful cases have reported in which vaccinees developed virulent wild type YFV infection. This efficacy is presumed to be result both neutralizing antibodies robust T cell response. However, particular immune components required for protection against never evaluated. An understanding mechanisms that underlie...
SARS-CoV-2 is a novel coronavirus that causes the acute respiratory disease-Coronavirus disease 2019 (COVID-19)-which has led to global health crisis. Currently, no prophylactics or therapies exist control virus spread mitigate disease. Thus, risk of infection for physicians and scientists high, requiring work be conducted in Biosafety Level-3 (BSL-3) facilities if will isolated propagated. However, inactivation can enable safe handling at reduced biosafety level, making samples accessible...
Asthma is an inflammatory condition for which anti-inflammatory glucocorticoids are the standard of care. However, similar efficacy has not been shown agents targeting cells and pathways. This suggests a noninflammatory cell contributor (e.g., epithelium) to asthmatic inflammation. Herein, we sought define intrinsic glucocorticoid-affected properties airway epithelium compared with normal epithelium. Human primary differentiated epithelia were cultured in glucocorticoid-free medium beginning...
Chronic airway diseases are characterized by inflammation and mucus overproduction. The MUC5AC mucin gene is upregulated the proinflammatory cytokine interleukin-1 β (IL-1β) via activation of cAMP response element-binding protein (CREB) in NCI-H292 cancer cell line nuclear factor-κB (NF-κB) HBE1 transformed line, with each transcription factor binding to a cognate cis site proximal or distal region, respectively, promoter. We utilized primary differentiated human bronchial epithelial (HBE)...
Hydractinia symbiolongicarpus, a colonial cnidarian, is tractable model system for many cnidarian-specific and general biological questions. Until recently, tests of gene function in have relied on laborious forward genetic approaches, randomly integrated transgenes, or transient knockdown mRNAs.Here, we report the use CRISPR/Cas9 genome editing to generate targeted genomic insertions H. symbiolonigcarpus. We used promote homologous recombination two fluorescent reporters, eGFP tdTomato,...
Abstract Biologic tissues are generally opaque due to optical properties that result in scattering and absorption of light. Preparation for microscopy often involves sectioning a thickness 50‐100 µm, the practical limits light penetration recovery. A researcher who wishes image whole tissue must acquire potentially hundreds individual sections before rendering them into three‐dimensional volume. Clearing removes strongly light‐scattering light‐absorbing components equalizes refractive index...
Abstract Age-related macular degeneration (AMD) is an expanding problem as longevity increases worldwide. While inflammation clearly contributes to vision loss in AMD, the mechanism remains controversial. Here we show that neutrophils are important this inflammatory process. In retinas of both early AMD patients and a mouse model with AMD-like phenotype, neutrophil infiltration. Such infiltration was confirmed experimentally using ribbon-scanning confocal microscopy (RSCM) IFNλ− activated...
Abstract Dengue virus (DENV) is a mosquito-borne flavivirus that causes serious global human disease and mortality. Skin immune cells are an important component of initial DENV infection systemic spread. Here, we show mast target in skin induces degranulation alters cytokine growth factor expression profiles. Importantly, to our knowledge, also demonstrate for the first time localizes within secretory granules infected cells. In addition, extracellular was infectious vitro vivo, trafficking...
The advancement of serial cryoFIB/SEM offers an opportunity to study large volumes near-native, fully hydrated frozen cells and tissues at voxel sizes 10 nm below. We explored this capability for pathologic characterization vitrified human patient by developing optimizing a volume imaging workflow. demonstrate profound disruption subcellular architecture in primary fibroblasts from Leigh syndrome harboring disease-causing mutation USMG5 protein responsible impaired mitochondrial energy production.
A growing community is constructing a next-generation file format (NGFF) for bioimaging to overcome problems of scalability and heterogeneity. Organized by the Open Microscopy Environment (OME), individuals institutes across diverse modalities facing these have designed specification process (OME-NGFF) address needs. This paper brings together wide range those members describe cloud-optimized itself -- OME-Zarr along with tools data resources available today increase FAIR access remove...
This research investigates the cyclic effect on highly thermal hybrid nano-graphene conductive ink. The goal is to develop and formulate a graphene ink by combining nano-graphene, silver flakes acetate as fillers which were mixed with chemical organic solvents. evaluates resistivity at room temperature before after bending test in terms of electrical mechanical properties. For this purpose, new formulation was developed using primary mixture sonicated stirred form powder then dripped...
While the molecular and cellular effects of opioids have been extensively studied, precise mechanisms by which these drugs target specific brain regions over time remain unclear. Similarly, despite well-documented sex differences in opioid responses, anatomical basis for this sexual dimorphism is not well characterized. To address questions, we developed an automated, scalable, unbiased approach whole-brain mapping neuronal activity marker c-Fos response to acute morphine exposure. Using...