A5031602962- Zebrafish Biomedical Research Applications
- Angiogenesis and VEGF in Cancer
- Congenital heart defects research
- Lymphatic System and Diseases
- Subterranean biodiversity and taxonomy
- Single-cell and spatial transcriptomics
- Retinal Development and Disorders
- Marine Ecology and Invasive Species
- Reproductive biology and impacts on aquatic species
- Fish Ecology and Management Studies
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- Planarian Biology and Electrostimulation
- Pregnancy and preeclampsia studies
- Hippo pathway signaling and YAP/TAZ
- Developmental Biology and Gene Regulation
- Chemokine receptors and signaling
- Cellular transport and secretion
- CRISPR and Genetic Engineering
- Protist diversity and phylogeny
- Cell Image Analysis Techniques
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Aquatic Invertebrate Ecology and Behavior
- TGF-β signaling in diseases
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2016-2025
National Institutes of Health
2016-2025
Health and Human Development (2HD) Research Network
2015-2024
Organogenesis (United States)
2007-2024
Korea Advanced Institute of Science and Technology
2019
IFOM
2017
Uppsala University
2017
Dartmouth College
2017
University of Maryland, College Park
2016
The blood-brain barrier is essential for the proper homeostasis and function of CNS, but its mechanism poorly understood. Perivascular cells surrounding brain blood vessels are thought to be important establishment, their roles not well defined. Here, we describe a novel perivascular cell population closely associated with on zebrafish brain. Based similarities in morphology, location, scavenger behavior, these appear equivalent variably characterized as Fluorescent Granular Perithelial...
Mural cells (vascular smooth muscle and pericytes) play a critical role in the development of vasculature, promoting vascular quiescence long-term vessel stabilization through their interactions with endothelial cells. However, mechanistic details how mural stabilize vessels are not fully understood. We have examined emergence functional investing dorsal aorta during early using zebrafish. Consistent previous literature, our data suggest that ensheathing emerge from sub-population adjacent...
Here, we show that a novel Rspo1-Wnt-Vegfc-Vegfr3 signaling pathway plays an essential role in developmental angiogenesis. A mutation R-spondin1 (rspo1), Wnt regulator, was uncovered during forward-genetic screen for angiogenesis-deficient mutants the zebrafish. Embryos lacking rspo1 or proposed receptor kremen form primary vessels by vasculogenesis, but are defective subsequent Endothelial cell-autonomous inhibition of canonical also blocks angiogenesis vivo. The pro-angiogenic effects...
The lymphatic vascular system is a hierarchically organized complex network essential for tissue fluid homeostasis, immune trafficking, and absorption of dietary fats in the human body. Despite its importance, assembly still not fully understood. zebrafish powerful model organism that enables study vessel development using high-resolution imaging sophisticated genetic experimental manipulation. Although several studies have described early fish, at later stages has been completely...
The preferential accumulation of vascular smooth muscle cells (vSMCs) on arteries versus veins during early development is a well-described phenomenon, but the molecular pathways underlying this polarization are not well understood. In zebrafish, cxcr4a receptor (mammalian CXCR4) and its ligand cxcl12b CXCL12) both preferentially expressed at time points consistent with arrival differentiation first vSMCs development. We show that autocrine cxcl12b/cxcr4 activity leads to increased...
Despite the growing popularity of zebrafish model system, optimal husbandry conditions for this animal are not well defined. The aim study was to examine effect stocking density on reproductive performance in zebrafish. In study, undertaken by eight different facilities, clutches at least 200 wild-type embryos from a single pairwise mating were produced each participating institution and subsequently reared according "in-house protocols" until they 14 weeks old. Fish then randomly assigned...
Abstract Biologic tissues are generally opaque due to optical properties that result in scattering and absorption of light. Preparation for microscopy often involves sectioning a thickness 50‐100 µm, the practical limits light penetration recovery. A researcher who wishes image whole tissue must acquire potentially hundreds individual sections before rendering them into three‐dimensional volume. Clearing removes strongly light‐scattering light‐absorbing components equalizes refractive index...
The recent discovery of meningeal lymphatics in mammals is reshaping our understanding fluid homeostasis and cellular waste management the brain, but visualization experimental analysis these vessels challenging mammals. Although optical clarity advantages zebrafish have made this an essential model organism for studying lymphatic development, existence has not yet been reported species.
Vessel formation has been extensively studied at the tissue level, but difficulty in imaging endothelium with cellular resolution hampered study of morphogenesis and behavior endothelial cells (EC) vivo. We are using endothelial-specific transgenes high-resolution to examine single zebrafish. By generating mosaics that simultaneously mark nuclei membranes we able definitively identify morphology individual EC during vessel sprouting lumen formation. Using these methods, show developing trunk...
During embryonic development, cell type-specific transcription factors promote identities, while epigenetic modifications are thought to contribute maintain these fates. Our understanding of how genetic and modes regulation work together establish cellular identity is still limited, however. Here, we show that DNA methyltransferase 3bb.1 (dnmt3bb.1) essential for maintenance hematopoietic stem progenitor (HSPC) fate as part an early Notch-runx1-cmyb HSPC specification pathway in the...
ZAP70 [zeta-chain (TCR)-associated protein kinase, 70-kDa], is required for T cell activation. deficiencies in humans and null mutations mice lead to severe combined immune deficiency. Here, we describe a zap70 loss-of-function mutation zebrafish (zap70y442) that was created using transcription activator-like effector nucleases (TALENs). In contrast what has been reported morphant zebrafish, zap70y442 homozygous mutant displayed normal development of blood lymphatic vasculature....
The zebrafish has become a widely used animal model due, in large part, to its accessibility and usefulness for high-resolution optical imaging. Although research historically focused mostly on early development, recent years the fish increasingly been study regeneration, cancer metastasis, behavior other processes taking place juvenile adult animals. However, imaging of live is extremely challenging, with survival limited few tens minutes using standard methods developed embryos larvae....
Lymph nodes and other secondary lymphoid organs play critical roles in immune surveillance activation mammals, but the deep internal locations of these make it challenging to image study them living animals. Here, we describe a previously uncharacterized external organ zebrafish ideally suited for studying cell dynamics vivo, axillary (ALO). This small, translucent has an outer cortex teeming with cells, inner medulla mesh-like network fibroblastic reticular cells along which migrate,...
Zebrafish (Danio rerio) is a valuable model organism for studying developmental processes due to its external development and the optical clarity of embryos larvae. However, as proceeds zebrafish form increasingly opaque tissues that impede visualization deep structures. Although tissue clearing methods have been applied facilitate imaging at these later stages, most limited ability clear dense such bone cartilage, cause significant morphological distortion, and/or result in loss fluorescent...
ABSTRACT The meninges are a set of connective tissue layers that surround the central nervous system, protecting brain from mechanical shock, supporting its buoyancy, guarding it infection and injury, maintaining homeostasis. Despite their critical role, molecular identity, developmental origins, functional properties cell types populating remain poorly characterized. This is in large part due to lack type specific markers difficulty visualizing studying these structures through thick...
Abstract Vestigial structures are key indicators of evolutionary descent, but the mechanisms underlying their development poorly understood. This study examines vestigial eye formation in teleost Astyanax mexicanus , which consists a sighted surface-dwelling morph and multiple populations blind cave morphs. Cavefish embryos initially develop eyes, they subsequently degenerate become embedded head. The mutated genes involved cavefish have not been characterized. Here we identify cystathionine...