A5054469374- Pregnancy and preeclampsia studies
- Birth, Development, and Health
- Neonatal Respiratory Health Research
- Microtubule and mitosis dynamics
- Genetics and Neurodevelopmental Disorders
- Advanced Fluorescence Microscopy Techniques
- Retinal Development and Disorders
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- RNA regulation and disease
- Neurogenesis and neuroplasticity mechanisms
- Advanced biosensing and bioanalysis techniques
- Optical Coherence Tomography Applications
- Neuroscience and Neuropharmacology Research
- Cell Image Analysis Techniques
University of Cincinnati
2018
Cincinnati Children's Hospital Medical Center
2016-2018
Abstract Biologic tissues are generally opaque due to optical properties that result in scattering and absorption of light. Preparation for microscopy often involves sectioning a thickness 50‐100 µm, the practical limits light penetration recovery. A researcher who wishes image whole tissue must acquire potentially hundreds individual sections before rendering them into three‐dimensional volume. Clearing removes strongly light‐scattering light‐absorbing components equalizes refractive index...
Healthy pregnancy depends on proper placentation—including proliferation, differentiation, and invasion of trophoblast cells—which, if impaired, causes placental ischemia resulting in intrauterine growth restriction preeclampsia. Mechanisms regulating invasion, however, are unknown. We report that reduction Inverted formin 2 (INF2) alters intracellular trafficking significantly impairs a model human extravillous trophoblasts. Furthermore, global loss Inf2 mice recapitulates maternal fetal...
Development of the mammalian forebrain requires a significant contribution from tubulin proteins to physically facilitate both large number mitoses in neurogenic brain (in form mitotic spindles) as well support cellular scaffolds guide radial migration (radial glial neuroblasts). Recent studies have identified mutations human genes affecting forebrain, including TUBB2B . We previously mouse mutation Tubb2b and we show here that mice heterozygous for this missense cognitive defects spatial...