A5039020080- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Angiogenesis and VEGF in Cancer
- Lymphatic System and Diseases
- Axon Guidance and Neuronal Signaling
- Epigenetics and DNA Methylation
- Developmental Biology and Gene Regulation
- Animal Genetics and Reproduction
- Microtubule and mitosis dynamics
- CRISPR and Genetic Engineering
- Hippo pathway signaling and YAP/TAZ
- Pregnancy and preeclampsia studies
- Subterranean biodiversity and taxonomy
- Fungal and yeast genetics research
- Fish Ecology and Management Studies
- Wnt/β-catenin signaling in development and cancer
- Renal and related cancers
- Single-cell and spatial transcriptomics
- Aquaculture disease management and microbiota
- Cancer-related gene regulation
- Cell Adhesion Molecules Research
- Acute Myeloid Leukemia Research
- Retinal Development and Disorders
- Physiological and biochemical adaptations
- Cancer, Hypoxia, and Metabolism
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2016-2025
National Institutes of Health
2016-2025
Organogenesis (United States)
2000-2024
Korea Advanced Institute of Science and Technology
2019
IFOM
2008-2017
Dartmouth College
2017
Uppsala University
2017
University of Maryland, College Park
2016
Health and Human Development (2HD) Research Network
2003-2015
Laboratory of Molecular Genetics
2006-2009
Recent evidence indicates that acquisition of artery or vein identity during vascular development is governed, in part, by genetic mechanisms. The artery-specific expression a number Notch signaling genes mouse and zebrafish suggests this pathway may play role arterial-venous cell fate determination development. We show loss embryos leads to molecular defects differentiation, including markers ectopic venous within the dorsal aorta. Conversely, we find activation repression fate. Finally,...
ABSTRACT As part of a large-scale mutagenesis screen the zebrafish genome, we have identified 58 mutations that affect formation and function cardiovascular system. The system is particularly amenable for screening in transparent embryo because heart blood vessels are prominent their easily examined. We classified affecting into those primarily either morphogenesis or function. Nine clearly disrupt heart. cloche deletes endocardium. In mutants, myocardial layer forms absence endocardium but...
ABSTRACT Endothelial and hematopoietic cells appear synchronously on the extra-embryonic membranes of amniotes in structures known as blood islands. This observation has led to suggestion that these two ventral lineages share a common progenitor. Recently, we have shown zebrafish, Danio rerio, single cell marginal zone early blastula can give rise both endothelial well other mesodermal (Stainier, D. Y. R., Lee, R. K. Fishman, M. C. (1993). Development 119, 31-40; K., Stainier, Weinstein, B....
We have used time-lapse multiphoton microscopy of living Tg(fli1:EGFP)y1 zebrafish embryos to examine how a patterned, functional network angiogenic blood vessels is generated in the early vertebrate trunk. Angiogenic vascular sprouts emerge from longitudinal trunk axial (the dorsal aorta and posterior cardinal vein) two spatially temporally distinct steps. Dorsal aorta-derived form an initial primary segments, followed by emergence vein-derived secondary that interact interconnect...
Summary: We report the generation and characterization of transgenic mouse zebrafish expressing green fluorescent protein (GFP) specifically in vascular endothelial cells a relatively uniform fashion. These reporter lines exhibit vessels developing embryos throughout adulthood, allowing visualization general patterns with single cell resolution. Furthermore, we show ability to purify from whole adult organs by step fluorescence activated sorting. expect that these reporters will be useful...
The first artery and vein of the vertebrate embryo assemble in trunk by migration coalescence angioblasts to form endothelial tubes. gridlock ( grl ) mutation zebrafish selectively perturbs assembly (the aorta). Here it is shown that encodes a basic helix-loop-helix (bHLH) protein belonging Hairy/Enhancer split family bHLH proteins. gene expressed lateral plate mesoderm before vessel formation, thereafter aorta not vein. These results suggest arterial identity established even onset blood...
The zebrafish mutant violet beauregarde (vbg) can be identified at two days post-fertilization by an abnormal circulation pattern in which most blood cells flow through a limited number of dilated cranial vessels and fail to perfuse the trunk tail. This phenotype cannot explained caudal vessel abnormalities or defect patterning, but instead stems from increase endothelial cell specific vessels. We show that vbg encodes activin receptor-like kinase 1 (Acvrl1; also known as Alk1), TGFbeta type...
Recent evidence indicates a specific role for vascular endothelial growth factor (Vegfa) during artery development in both zebrafish and mouse embryos, whereas less is known about signals that govern vein formation. In zebrafish, loss of vegfa blocks segmental formation reduces artery-specific gene expression, veins are largely unaffected. Here, we describe mutation the vegf receptor-2 homolog, kdra , which eliminates its kinase activity leads to defects development. We further find Flt4,...