A5047774612- Lymphatic System and Diseases
- MicroRNA in disease regulation
- Congenital heart defects research
- Zebrafish Biomedical Research Applications
- RNA Research and Splicing
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Traumatic Brain Injury and Neurovascular Disturbances
- Airway Management and Intubation Techniques
- Cerebral Venous Sinus Thrombosis
- Emergency and Acute Care Studies
- Acute Kidney Injury Research
- Trauma Management and Diagnosis
- Planarian Biology and Electrostimulation
- Trauma and Emergency Care Studies
- Angiogenesis and VEGF in Cancer
- Sympathectomy and Hyperhidrosis Treatments
- Sepsis Diagnosis and Treatment
- Pregnancy and preeclampsia studies
- Body Contouring and Surgery
- Ophthalmology and Eye Disorders
- Restraint-Related Deaths
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Viral Infectious Diseases and Gene Expression in Insects
- Infectious Encephalopathies and Encephalitis
University of Illinois Chicago
2023-2025
Illinois College
2025
Inha University
2010-2022
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2016-2020
National Institutes of Health
2016-2020
Korea Advanced Institute of Science and Technology
2019
New York University
2018
Bristol-Myers Squibb (South Korea)
2012-2014
University of Florida
2010-2014
Florida College
2012
The blood-brain barrier is essential for the proper homeostasis and function of CNS, but its mechanism poorly understood. Perivascular cells surrounding brain blood vessels are thought to be important establishment, their roles not well defined. Here, we describe a novel perivascular cell population closely associated with on zebrafish brain. Based similarities in morphology, location, scavenger behavior, these appear equivalent variably characterized as Fluorescent Granular Perithelial...
MicroRNAs (miRNAs) are small non-coding RNAs that posttranscriptionally regulate gene expression during many biological processes. Recently, the aberrant expressions of miRNAs have become a major focus in cancer research. The purpose this study was to identify deregulated oral and further on specific were related patient survival. Here, we report miRNA profiling provided more precise information when squamous cell carcinomas subcategorized basis clinicopathological parameters (tumor primary...
MicroRNAs (miRNAs) are small, noncoding RNAs involved in posttranscriptional regulation of protein-coding genes various biological processes. In our preliminary miRNA microarray analysis, miR-375 was identified as the most underexpressed human oral tumor versus controls. The purpose present study is to examine function a candidate suppressor cancer. Cancerous inhibitor PP2A (CIP2A), guardian oncoprotein MYC, target based on bioinformatics. Luciferase assay accompanied by sequence mutagenesis...
While microRNAs (miRNAs) are extensively studied in post-transcriptional regulation of gene expressions many biological processes, cellular miRNA-mediated viral genes remains unclear. In particular, the interplay between human papillomavirus (HPV) and miRNAs how these interactions contribute to HPV-associated cancers remain elusive. Transient transfection miR-375-mimic was used compensate loss-of-function miR-375 HPV-positive cancer. Regulation oncogenic molecules their downstream via cancer...
The lymphatic vascular system is a hierarchically organized complex network essential for tissue fluid homeostasis, immune trafficking, and absorption of dietary fats in the human body. Despite its importance, assembly still not fully understood. zebrafish powerful model organism that enables study vessel development using high-resolution imaging sophisticated genetic experimental manipulation. Although several studies have described early fish, at later stages has been completely...
The recent discovery of meningeal lymphatics in mammals is reshaping our understanding fluid homeostasis and cellular waste management the brain, but visualization experimental analysis these vessels challenging mammals. Although optical clarity advantages zebrafish have made this an essential model organism for studying lymphatic development, existence has not yet been reported species.
Objectives: Hyperglycemia and hypokalemia are common problems in patients with aneurysmal subarachnoid hemorrhage (aSAH). The aim of this study was to determine whether the plasma glucose potassium ratio (GPR) predicts mortality due aSAH. Methods: We prospectively recruited aSAH healthy controls between March 2007 May 2017. Clinical outcomes included poor outcome (modified Rankin scale score 3-6) after 3 months. Multivariable analysis used association GPR 3-month patients. Results: A total...
Shoc2 is a scaffold protein critical for regulating Raf-1 kinase activation and the amplitude of ERK1/2 signals. Hereditary mutations result in Noonan Syndrome with Loose anagen Hair (NSLH), debilitating congenital disorder associated lymphatic abnormalities. We demonstrate that zebrafish null larvae exhibit nearly complete loss vasculature, suggesting gene plays positive role during developmental lymphangiogenesis. The lymphatics caused by deficiency can be rescued endothelial autonomous...
Immortalization is an early and essential step of human carcinogenesis which associated with alterations in gene expression regulation. Suppression subtractive hybridization (SSH) was successfully performed to identify immortalization-associated genes upregulated SV40-immortalized lung fibroblasts. We identified 116 known were related diverse functions, 32.8% relevant for cell cycle or proliferation indicating the potential involvement these immortalization. chose eight located on...
MicroRNA (miRNA)-mediated gene regulation has become a major focus in many biological processes. GW182 and its long isoform TNGW1 are marker proteins of GW/P bodies bind to Argonaute the RNA induced silencing complex. The goal this study is further define distinguish repression domain(s) human GW182/TNGW1. Two non-overlapping regions, Δ12 (amino acids 896–1219) containing Ago hook Δ5 1670–1962) RRM, both comparable tethering assay. Mapping data showed that RRM flanking sequences Δ5, but not...
Head and neck cancer is one of the deadliest malignant diseases chemotherapy a common treatment option. Despite development chemotherapies for several decades, how these drugs affect dynamics gene regulation still largely unknown. In our previous study, miR-375 was shown to be underexpressed in oral cancers thus unable serve as tumor suppressor microRNA regulate certain putative oncogenes. this we found that anti-cancer reactivated tongue cells. Incubation cells CAL 27 SCC-25 medium...
The post-transcriptional mechanisms contributing to molecular regulation of developmental lymphangiogenesis and lymphatic network assembly are not well understood. MicroRNAs important regulators during development. Here, we use high throughput small RNA sequencing identify miR-204, a highly conserved microRNA dramatically enriched in vs. blood endothelial cells human zebrafish. Suppressing miR-204 leads loss vessels while overproduction accelerates vessel formation, suggesting critical...
This study aimed to report the survival of a rare case complete tracheal transection followed by blunt neck trauma.A 66-year-old man was presented in emergency room after motorcycle accident which rope wrapped around his neck.Although alert, he respiratory distress.A computed tomographic scan showed cervical trachea.Emergency exploration revealed that laceration had been cut from anterior third ring posterior first and esophageal wall ruptured.Laryngectomy, tracheostomy, esophagopharyngeal...
Ras proteins regulate signaling pathways that control many cellular responses, such as proliferation, survival, and differentiation. However, there are intriguing questions about the relationship between developmental timing of specific mutations resultant phenotypes in individual cells. In this study, we used Cre/loxP system for maintaining transgenic zebrafish lines harboring oncogenic Kras(V12) under nestin promoter, investigated effects activation neural progenitor Activated human was...
Abstract In regulation of the developmental process, balance between cellular proliferation and cell death is critical. Placental development tightly controls this mechanism, increased apoptosis placental trophoblasts can cause a variety gynecological diseases. Members immortalization‐upregulated protein (IMUP) family are nuclear proteins implicated in SV40‐mediated immortalization proliferation; however, mechanisms by which their expression regulated still unknown. We compared IMUP‐2 normal...
Acute kidney injury (AKI) is a common complication in patients with sepsis. We evaluated the potential prognostic value of plasma presepsin to predict AKI sepsis emergency department. A total 193 diagnosed based on Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3) were included this observational study. was defined according Kidney Disease Improving Global Outcomes clinical practice guideline. Plasma levels measured admission compared between who did those not...
Since its introduction, the zebrafish has provided an important reference system to model and study cardiovascular development as well lymphangiogenesis in vertebrates. A scientific workshop held at 2018 European Zebrafish Principal Investigators Meeting Trento, Italy chaired by Massimo Santoro, focused on most recent methods studies cardiac, vascular lymphatic development. Daniela Panáková Natascia Tiso described new molecular mechanisms signaling pathways involved cardiac differentiation...