A5011458972- Systemic Lupus Erythematosus Research
- Monoclonal and Polyclonal Antibodies Research
- Systemic Sclerosis and Related Diseases
- Inflammatory Myopathies and Dermatomyositis
- T-cell and B-cell Immunology
- RNA Research and Splicing
- Immunodeficiency and Autoimmune Disorders
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Lymphoma Diagnosis and Treatment
- Celiac Disease Research and Management
- COVID-19 Clinical Research Studies
- RNA regulation and disease
- Chronic Lymphocytic Leukemia Research
- Liver Diseases and Immunity
- Renal Diseases and Glomerulopathies
- Dermatologic Treatments and Research
- Cellular transport and secretion
- Mast cells and histamine
- Rheumatoid Arthritis Research and Therapies
- Diabetes and associated disorders
- Skin Diseases and Diabetes
- Sepsis Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- Autoimmune Bullous Skin Diseases
University of Calgary
2016-2025
Health Sciences Centre
1984-2024
Deleted Institution
2023
Scleroderma Foundation
2022
Moncton Hospital
2022
McGill University Health Centre
2022
Jewish General Hospital
2014-2021
University of Toronto
1989-2021
McGill University
2014-2021
Mayo Clinic in Florida
2021
To develop new classification criteria for systemic lupus erythematosus (SLE) jointly supported by the European League Against Rheumatism (EULAR) and American College of Rheumatology (ACR).This international initiative had four phases. 1) Evaluation antinuclear antibody (ANA) as an entry criterion through systematic review meta-regression literature generation Delphi exercise, early patient cohort, a survey. 2) Criteria reduction nominal group technique exercises. 3) definition weighting...
Stress granules (SGs) are cytoplasmic aggregates of stalled translational preinitiation complexes that accumulate during stress. GW bodies/processing bodies (PBs) distinct sites mRNA degradation. In this study, we show SGs and PBs spatially, compositionally, functionally linked. induced by stress, but SG assembly requires eIF2α phosphorylation, whereas PB does not. They also dispersed inhibitors elongation share several protein components, including Fas-activated serine/threonine...
Abstract This study reports the identification of an autoantibody in sera some patients with systemic lupus erythematosus that reacted nuclear antigen(s) proliferating cells. The was initially detected by observation it did not react immunofluorescence nuclei renal tubular or glomerular cells, nor hepatic parenchymal but only scattered cells interstitial tissue these two organs. In contrast, many lymphocytes lymph node follicles, spleen, and thymus this antinuclear antibody. Normal...
Abstract Objective . To determine the range of antinuclear antibodies (ANA) in “healthy” individuals compared with that patients systemic lupus erythematosus (SLE), sclerosis (SSc; scleroderma), Sjögren's syndrome (SS), rheumatoid arthritis (RA), or soft tissue rheumatism (STR). Methods Fifteen international laboratories experienced performing tests for ANA by indirect immunofluorescence participated analyzing coded sera from healthy and 5 different disease groups described above. Except...
Sera from patients with scleroderma contained several autoantibodies to nuclear antigens which were distinguished by different patterns of immunofluorescence staining. One these reacted centromeric regions chromosomes. In chromosome spreads, the staining appeared as two small spheres at centromere, resembling kinetochores. The antigenic determinant be a protein or polypeptide tightly bound DNA. autoantibody was reactive centromeres cells derived humans, mice, and Chinese hamsters. present in...
Anti-nuclear antibodies (ANA) are fundamental for the diagnosis of autoimmune diseases, and have been determined by indirect immunofluorescence assay (IIFA) decades. As demand ANA testing increased, alternative techniques were developed challenging classic IIFA. These platforms differ in their antigen profiles, sensitivity specificity, raising uncertainties regarding standardisation interpretation incongruent results. Therefore, an international group experts has created recommendations...
Abstract Objective To identify in patients with Raynaud's phenomenon (RP) independent markers that predict progression to definite systemic sclerosis (SSc) and determine SSc the type sequence of microvascular damage its relationship SSc‐specific autoantibodies. Methods Consecutive referred for evaluation RP who had no connective tissue disease were evaluated by nailfold capillary microscopy (NCM) anticentromere (anti–CENP‐B), anti‐Th/To, anti–topoisomerase I, anti–RNA polymerase III...
Abstract Antinuclear antibodies wer demonstrated in the sera of 43 45 (96%) patients with progressive systemic sclerosis (22 24 diffuse scleroderma and 21 CREST syndrome variant). This high percentage positive reactions resulted from use a tissue culture substrate (Hep‐2) to detect antinuclear by indirect immunofluorescent method. Patterns nuclear staining included fine speckles, large coarse nucleolar centromere staining. When organ sections such as mouse kidney were used for detection...
The indirect immunofluorescence assay (IIFA) on HEp-2 cells is widely used for detection of antinuclear antibodies (ANA). dichotomous outcome, negative or positive, integrated in diagnostic and classification criteria several systemic autoimmune diseases. However, the IIFA test has much more to offer: besides titre fluorescence intensity, it also provides pattern(s). latter include nucleus cytoplasm interphase as well patterns associated with mitotic cells. International Consensus ANA...
Abstract Objective To determine the safety of rituximab, to provide preliminary data regarding potential efficacy and investigate effects rituximab on autoimmunity fibrosis in patients with diffuse cutaneous systemic sclerosis (dcSSc). Methods Fifteen dcSSc, all whom experienced their first non–Raynaud's disease–associated disease manifestation within 18 months trial entry, were recruited receive 2 intravenous doses (1,000 mg), administered weeks apart. Safety, clinical, exploratory outcomes...
Abstract Although critical for host defense, innate immune cells are also pathologic drivers of acute respiratory distress syndrome (ARDS). Innate dynamics during Coronavirus Disease 2019 (COVID-19) ARDS, compared to ARDS from other pathogens, is unclear. Moreover, mechanisms underlying the beneficial effects dexamethasone severe COVID-19 remain elusive. Using single-cell RNA sequencing and plasma proteomics, we discovered that, bacterial was associated with expansion distinct neutrophil...
A novel human cellular structure has been identified that contains a unique autoimmune antigen and multiple messenger RNAs. This complex was discovered using an serum from patient with motor sensory neuropathy protein of 182 kDa. The gene cDNA encoding the indicated open reading frame glycine-tryptophan (GW) repeats single RNA recognition motif. Both patient's rabbit raised against recombinant GW costained discrete cytoplasmic speckles designated as bodies (GWBs) do not overlap Golgi...
Abstract We have identified a novel .ca 400 kDa cell‐cycle dependent kinetochore associated protein in human cells, designated CENP‐F, using autoimmune serum. Immunofluorescence staining the native serum, affinity purified antibodies, or antibodies raised against cloned portion of CENP‐F first reveals homogeneously distributed throughout nucleus HeLa cells G2 stage cell cycle. Progression into prophase is accompanied by localization to all regions karyotype. Kinetochore association...
A novel cytoplasmic compartment referred to as GW bodies was initially identified using human autoantibodies a 182 kDa protein named GW182. are small, generally spherical, domains that vary in number and size several mammalian cell types examined date. Based on our earlier studies, were proposed be sites for mRNA storage and/or degradation. In the present study, immunogold electron microscopy dense structures of 100-300 nm diameter devoid lipid bilayer membrane. These appeared comprise...