A5002554504- Monoclonal and Polyclonal Antibodies Research
- Systemic Lupus Erythematosus Research
- Immunodeficiency and Autoimmune Disorders
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Science and Education Research
- Immune Cell Function and Interaction
- T-cell and Retrovirus Studies
- Cytomegalovirus and herpesvirus research
- Mosquito-borne diseases and control
- Biochemical and Molecular Research
- Blood groups and transfusion
- Education and Digital Technologies
- Diabetes and associated disorders
- Vibrio bacteria research studies
- Eosinophilic Disorders and Syndromes
- Moringa oleifera research and applications
- Natural product bioactivities and synthesis
- Medical Device Sterilization and Disinfection
- Aquaculture disease management and microbiota
- Autoimmune and Inflammatory Disorders Research
- Immunotherapy and Immune Responses
- Autoimmune Bullous Skin Diseases
- Biosimilars and Bioanalytical Methods
Pontifícia Universidade Católica de Goiás
2016-2025
Universidade Federal de São Paulo
2008-2015
Union des Industries Ferroviaires Européennes
2010
Universidade Federal de Goiás
2009
The indirect immunofluorescence assay (IIFA) on HEp-2 cells is widely used for detection of antinuclear antibodies (ANA). dichotomous outcome, negative or positive, integrated in diagnostic and classification criteria several systemic autoimmune diseases. However, the IIFA test has much more to offer: besides titre fluorescence intensity, it also provides pattern(s). latter include nucleus cytoplasm interphase as well patterns associated with mitotic cells. International Consensus ANA...
O sistema imunológico é constituído por uma intrincada rede de órgãos, células e moléculas, tem finalidade manter a homeostase do organismo, combatendo as agressões em geral. A imunidade inata atua conjunto com adaptativa caracteriza-se pela rápida resposta à agressão, independentemente estímulo prévio, sendo primeira linha defesa organismo. Seus mecanismos compreendem barreiras físicas, químicas biológicas, componentes celulares moléculas solúveis. organismo frente um dano tecidual envolve...
The International Consensus on ANA Patterns (ICAP) was initiated as a workshop aiming to thoroughly discuss and achieve consensus regarding the morphological patterns observed in indirect immunofluorescence assay HEp-2 cells. One of topics discussed at second ICAP workshop, addressed this paper, harmonization reporting test results. This discussion centered issue if cytoplasmic mitotic should be reported positive or negative. report outlines issues that impact two major different methods....
The second meeting for the International Consensus on Antinuclear antibody (ANA) Pattern (ICAP) was held 22 September 2015, one day prior to opening of 12th Dresden Symposium Autoantibodies in Dresden, Germany. ultimate goal ICAP is promote harmonization and understanding autoantibody nomenclature, thereby optimizing ANA usage patient care. newly developed website www.ANApatterns.org introduced more than 50 participants. This followed by several presentations discussions focusing key issues...
The establishment of the International Consensus on ANA Patterns (ICAP) in 2014-2015 was welcomed by members medical community as a significant improvement guiding harmonization test interpretation and reporting. In subsequent years, several itinerant meetings continuous interaction with contributed to disseminate ICAP immunofluorescence patterns observed indirect assay HEp-2 cells (HEp-2 IFA) promote progressive classification IFA patterns. 6th Workshop held person September 6, 2021...
Abstract The indirect immunofluorescence assay (IFA) on HEp-2 cells is the reference method for autoantibody screening. IFA pattern provides useful information possible autoantibodies in sample. International Consensus Antinuclear Antibody Patterns (ICAP) initiative seeks to define and harmonize nomenclature of patterns. most relevant usual patterns have been assigned an alphanumeric code from anti-cell (AC)-1 AC-28 were organized into a classification algorithm (www.ANApatterns.org)....
O IV Consenso Brasileiro para Pesquisa de Autoanticorpos em Células HEp-2 (FAN) realizado Vitória (ES), no dia 18 setembro 2012, objetivou discutir es- tratégias e recomendações relacionadas ao procedimento técnico, à padronização interpretação dos resultados da pesquisa autoanticorpos células HEp-2. Participaram do evento 23 pesquisadores especialistas Universidades laboratórios brasileiros. Foram abordados diferentes tópicos, discutidos amplamente a fim se estabelecer específicas. integrou...
OBJETIVO:O 3º Consenso Brasileiro para pesquisa de autoanticorpos em Células HEp-2 (FAN) teve como propósito avaliar as dificuldades implantação do 2º ocorrido no ano 2002, discutir estratégias controlar a qualidade ensaio e promover atualização das associações clínicas dos diversos padrões. MÉTODOS:Participaram encontro Goiânia nos dias 13 14 abril 2008 pesquisadores especialistas centros universitários laboratórios clínicos diferentes regiões Brasil, com o aprovar recomendações que visam à...
The International Consensus on Antinuclear Antibody (ANA) Patterns (ICAP) has recently proposed nomenclature in order to harmonize ANA indirect immunofluorescence (IIF) pattern reporting. ICAP distinguishes competent-level from expert-level patterns. A survey was organized evaluate reporting, familiarity, and considered clinical value of IIF patterns.Two surveys were distributed by European Autoimmunity Standardization Initiative (EASI) working groups, the UK NEQAS laboratory professionals...
O sistema imunológico é constituído por uma intrincada rede de órgãos, células e moléculas, tem finalidade manter a homeostase do organismo, combatendo as agressões em geral. A imunidade inata atua conjunto com adaptativa caracteriza-se pela rápida resposta à agressão, independentemente estímulo prévio, sendo primeira linha defesa organismo. Seus mecanismos compreendem barreiras físicas, químicas biológicas, componentes celulares moléculas solúveis. organismo frente um dano tecidual envolve...
The identification of regulatory T cells (Treg cells) as CD4(+)CD25(high) may be upset by the increased frequency activated effector (Teff in inflammatory diseases such systemic lupus erythematosus (SLE). This study aimed to evaluate T-cell subsets according expression CD25 and CD127 active (A-SLE) inactive SLE (I-SLE).Peripheral blood mononuclear (PBMCs) from 26 A-SLE patients (SLE Disease Activity Index (SLEDAI) = 10.17 ± 3.7), 31 I-SLE (SLEDAI 0), healthy controls (HC) were analysed...
OBJETIVO: O Segundo Consenso Brasileiro de Fator Antinuclear (FAN) em Células HEp-2 ratificou os algoritmos decisão para leitura dos padrões do FAN na imunofluorescência indireta vistos primeira edição Brasileiro, adicionando ainda um novo algoritmo relacionado com mistos. MÉTODOS: Tendo vista a habilidade teste detectar autoantígenos nos distintos compartimentos celulares, e não apenas no núcleo, propõe-se novas denominações este exame laboratorial. RESULTADOS E CONCLUSÕES: Como algumas...
O sistema imunológico é constituído por uma intrincada rede de órgãos, células e moléculas tem finalidade manter a homeostase do organismo, combatendo as agressões em geral.A imunidade inata atua conjunto com adaptativa caracteriza-se pela rápida resposta à agressão, independentemente estímulo prévio, sendo primeira linha defesa organismo.Seus mecanismos compreendem barreiras físicas, químicas biológicas, componentes celulares solúveis.A organismo frente um dano tecidual envolve diversas...
Abstract Background The V Brazilian Consensus for determination of autoantibodies against cellular constituents on HEp-2 cells, held in Brasilia (DF, Brazil) August 27, 2016, discussed the harmonization between ANA (BCA) guidelines and International Patterns (ICAP) recommendations ( www.anapatterns.org ). Initial were formulated by group experts with purpose guiding enabling clinical laboratories to adopt provide a common standard national international consensuses. Mainbody Twenty...
O sistema imune sadio deve manter o balanço entre a capacidade de responder agentes infecciosos e sustentar autotolerância. A ausência resposta adequada submete indivíduo aos efeitos deletérios da invasão por patógenos, ao passo que respondendo modo exacerbado pode gerar respostas inflamatórias prejudiciais. Acreditava-se os mecanismos deleção clonal anergia fossem essenciais no controle clones linfócitos T auto-reativos. Apesar das evidências funcionais favor existência células supressoras,...
INTRODUCTION: Indirect immunofluorescence on HEp-2 cells is considered the gold standard for detection of autoantibodies against cellular antigens. However, culture conditions, cell fixation and permeabilization processes interfere directly in preservation spatial distribution Therefore, one can assume that certain peculiarities processing substrate may affect recognition indirect patterns associated with several autoantibodies. OBJECTIVE: To evaluate a panel serum samples representing...
Rheumatoid Arthritis (RA) is a chronic inflammatory condition affecting the joints, leading to progressive destruction of cartilage and bones. This multifaceted complex disease involves processes such as continuous inflammation, angiogenesis, degradation, bone erosion in its pathogenesis. Adipocytes, which are cells present adipose tissue, play crucial role RA by secreting adipokines, bioactive proteins immunological molecules that can mediate inflammation immune response. Among most studied...